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Protein

Apoptotic protease-activating factor 1

Gene

Apaf1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ApoptosisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptotic protease-activating factor 1UniRule annotation
Short name:
APAF-1UniRule annotation
Gene namesi
Name:Apaf1Imported
ORF Names:mCG_10338Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1306796. Apaf1.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Expressioni

Gene expression databases

ExpressionAtlasiG3XA09. baseline and differential.

Interactioni

Subunit structurei

Monomer. Oligomerizes upon binding of cytochrome c and dATP.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020157.

Structurei

3D structure databases

SMRiG3XA09. Positions 1-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9090CARDInterPro annotationAdd
BLAST
Domaini600 – 1238639WD_REPEATS_REGIONInterPro annotationAdd
BLAST

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, WD repeatSAAS annotation

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
KOG4658. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00810000125363.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR017251. Apaf-1.
IPR024977. Apc4_WD40_dom.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 8 hits.
[Graphical view]
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G3XA09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAKARNCLL QHREALEKDI KTSYIMDHMI SNGVLSVIEE EKVKSQATQY
60 70 80 90 100
QRAAALIKMI LNKDNCAYIS FYNALLHEGY KDLAALLQSG LPLVSSSSVR
110 120 130 140 150
TVLCEGGVPQ RPVIFVTRKK LVHAIQQKLW KLNGEPGWVT IYGMAGCGKS
160 170 180 190 200
VLAAEAVRDH SLLEGCFSGG VHWVSIGKQD KSGLLMKLQN LCMRLDQEES
210 220 230 240 250
FSQRLPLNIE EAKDRLRVLM LRKHPRSLLI LDDVWDPWVL KAFDNQCQIL
260 270 280 290 300
LTTRDKSVTD SVMGPKHVVP VESGLGREKG LEILSLFVNM KKEDLPAEAH
310 320 330 340 350
SIIKECKGSP LVVSLIGALL RDFPNRWAYY LRQLQNKQFK RIRKSSSYDY
360 370 380 390 400
EALDEAMSIS VEMLREDIKD YYTDLSILQK DVKVPTKVLC VLWDLETEEV
410 420 430 440 450
EDILQEFVNK SLLFCNRNGK SFCYYLHDLQ VDFLTEKNRS QLQDLHRKMV
460 470 480 490 500
TQFQRYYQPH TLSPDQEDCM YWYNFLAYHM ASANMHKELC ALMFSLDWIK
510 520 530 540 550
AKTELVGPAH LIHEFVAYRH ILDEKDCAVC ENFQEFLSLN GHLLGRQPFP
560 570 580 590 600
NIVQLGLCEP ETSEVYRQAK LQAKQEGDTG RLYLEWINKK TIKNLSRLVV
610 620 630 640 650
RPHTDAVYHA CFSQDGQRIA SCGADKTLQV FKAETGEKLL DIKAHEDEVL
660 670 680 690 700
CCAFSSDDSY IATCSADKKV KIWDSATGKL VHTYDEHSEQ VNCCHFTNKS
710 720 730 740 750
NHLLLATGSN DFFLKLWDLN QKECRNTMFG HTNSVNHCRF SPDDELLASC
760 770 780 790 800
SADGTLRLWD VRSANERKSI NVKRFFLSSE DPPEDVEVIV KCCSWSADGD
810 820 830 840 850
KIIVAAKNKV LLFDIHTSGL LAEIHTGHHS TIQYCDFSPY DHLAVIALSQ
860 870 880 890 900
YCVELWNIDS RLKVADCRGH LSWVHGVMFS PDGSSFLTAS DDQTIRVWET
910 920 930 940 950
KKVCKNSAIV LKQEIDVVFQ ENETMVLAVD NIRGLQLIAG KTGQIDYLPE
960 970 980 990 1000
AQVSCCCLSP HLEYVAFGDE DGAIKIIELP NNRVFSSGVG HKKAVRHIQF
1010 1020 1030 1040 1050
TADGKTLISS SEDSVIQVWN WQTGDYVFLQ AHQETVKDFR LLQDSRLLSW
1060 1070 1080 1090 1100
SFDGTVKVWN VITGRIERDF TCHQGTVLSC AISSDATKFS STSADKTAKI
1110 1120 1130 1140 1150
WSFDLLSPLH ELKGHNGCVR CSAFSLDGIL LATGDDNGEI RIWNVSDGQL
1160 1170 1180 1190 1200
LHSCAPISVE EGTATHGGWV TDVCFSPDSK TLVSAGGYLK WWNVATGDSS
1210 1220 1230
QTFYTNGTNL KKIHVSPDFR TYVTVDNLGI LYILQVLE
Length:1,238
Mass (Da):139,924
Last modified:November 16, 2011 - v1
Checksum:iC08DDA906EE45476
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC138719 Genomic DNA. No translation available.
AC140410 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21522.1.
CH466539 Genomic DNA. Translation: EDL21525.1.
RefSeqiNP_001269876.1. NM_001282947.1.
UniGeneiMm.220289.

Genome annotation databases

EnsembliENSMUST00000162618; ENSMUSP00000124134; ENSMUSG00000019979.
GeneIDi11783.
UCSCiuc007gti.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC138719 Genomic DNA. No translation available.
AC140410 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21522.1.
CH466539 Genomic DNA. Translation: EDL21525.1.
RefSeqiNP_001269876.1. NM_001282947.1.
UniGeneiMm.220289.

3D structure databases

SMRiG3XA09. Positions 1-575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000162618; ENSMUSP00000124134; ENSMUSG00000019979.
GeneIDi11783.
UCSCiuc007gti.1. mouse.

Organism-specific databases

CTDi317.
MGIiMGI:1306796. Apaf1.

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
KOG4658. Eukaryota.
ENOG410XP3K. LUCA.
GeneTreeiENSGT00810000125363.

Miscellaneous databases

ChiTaRSiApaf1. mouse.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiG3XA09. baseline and differential.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
2.130.10.10. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR017251. Apaf-1.
IPR024977. Apc4_WD40_dom.
IPR001315. CARD.
IPR011029. DEATH-like_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00619. CARD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF00400. WD40. 8 hits.
[Graphical view]
PIRSFiPIRSF037646. Apop_pept_activating-1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 9 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome."
    Mural R.J., Adams M.D., Myers E.W., Smith H.O., Miklos G.L., Wides R., Halpern A., Li P.W., Sutton G.G., Nadeau J., Salzberg S.L., Holt R.A., Kodira C.D., Lu F., Chen L., Deng Z., Evangelista C.C., Gan W.
    , Heiman T.J., Li J., Li Z., Merkulov G.V., Milshina N.V., Naik A.K., Qi R., Shue B.C., Wang A., Wang J., Wang X., Yan X., Ye J., Yooseph S., Zhao Q., Zheng L., Zhu S.C., Biddick K., Bolanos R., Delcher A.L., Dew I.M., Fasulo D., Flanigan M.J., Huson D.H., Kravitz S.A., Miller J.R., Mobarry C.M., Reinert K., Remington K.A., Zhang Q., Zheng X.H., Nusskern D.R., Lai Z., Lei Y., Zhong W., Yao A., Guan P., Ji R.R., Gu Z., Wang Z.Y., Zhong F., Xiao C., Chiang C.C., Yandell M., Wortman J.R., Amanatides P.G., Hladun S.L., Pratts E.C., Johnson J.E., Dodson K.L., Woodford K.J., Evans C.A., Gropman B., Rusch D.B., Venter E., Wang M., Smith T.J., Houck J.T., Tompkins D.E., Haynes C., Jacob D., Chin S.H., Allen D.R., Dahlke C.E., Sanders R., Li K., Liu X., Levitsky A.A., Majoros W.H., Chen Q., Xia A.C., Lopez J.R., Donnelly M.T., Newman M.H., Glodek A., Kraft C.L., Nodell M., Ali F., An H.J., Baldwin-Pitts D., Beeson K.Y., Cai S., Carnes M., Carver A., Caulk P.M., Center A., Chen Y.H., Cheng M.L., Coyne M.D., Crowder M., Danaher S., Davenport L.B., Desilets R., Dietz S.M., Doup L., Dullaghan P., Ferriera S., Fosler C.R., Gire H.C., Gluecksmann A., Gocayne J.D., Gray J., Hart B., Haynes J., Hoover J., Howland T., Ibegwam C., Jalali M., Johns D., Kline L., Ma D.S., MacCawley S., Magoon A., Mann F., May D., McIntosh T.C., Mehta S., Moy L., Moy M.C., Murphy B.J., Murphy S.D., Nelson K.A., Nuri Z., Parker K.A., Prudhomme A.C., Puri V.N., Qureshi H., Raley J.C., Reardon M.S., Regier M.A., Rogers Y.H., Romblad D.L., Schutz J., Scott J.L., Scott R., Sitter C.D., Smallwood M., Sprague A.C., Stewart E., Strong R.V., Suh E., Sylvester K., Thomas R., Tint N.N., Tsonis C., Wang G., Wang G., Williams M.S., Williams S.M., Windsor S.M., Wolfe K., Wu M.M., Zaveri J., Chaturvedi K., Gabrielian A.E., Ke Z., Sun J., Subramanian G., Venter J.C., Pfannkoch C.M., Barnstead M., Stephenson L.D.
    Science 296:1661-1671(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MixedImported.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MixedImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Ensembl
    Submitted (SEP-2011) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: C57BL/6JImported.

Entry informationi

Entry nameiG3XA09_MOUSE
AccessioniPrimary (citable) accession number: G3XA09
Entry historyi
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: June 8, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.