UniProtKB - G3X9K3 (BIG1_MOUSE)
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Protein
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Gene
Arfgef1
Organism
Mus musculus (Mouse)
Status
Functioni
Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).By similarity
Enzyme regulationi
Inhibited by brefeldin A.By similarity
GO - Molecular functioni
- ARF guanyl-nucleotide exchange factor activity Source: GO_Central
- guanyl-nucleotide exchange factor activity Source: MGI
- myosin binding Source: MGI
- protein kinase A regulatory subunit binding Source: UniProtKB
GO - Biological processi
- endomembrane system organization Source: UniProtKB
- Golgi organization Source: UniProtKB
- negative regulation of actin filament polymerization Source: UniProtKB
- negative regulation of GTPase activity Source: UniProtKB
- neuron projection development Source: Ensembl
- positive regulation of protein glycosylation in Golgi Source: UniProtKB
- positive regulation of protein kinase B signaling Source: Ensembl
- positive regulation of wound healing Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of ARF protein signal transduction Source: InterPro
- regulation of establishment of cell polarity Source: UniProtKB
- vesicle-mediated transport Source: GO_Central
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Brefeldin A-inhibited guanine nucleotide-exchange protein 1Short name: BIG1 Short name: Brefeldin A-inhibited GEP 1 Alternative name(s): ADP-ribosylation factor guanine nucleotide-exchange factor 1 |
Gene namesi | Name:Arfgef1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442988. Arfgef1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000419330 | 1 – 1846 | Brefeldin A-inhibited guanine nucleotide-exchange protein 1Add BLAST | 1846 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 286 | PhosphoserineCombined sources | 1 | |
Modified residuei | 289 | PhosphoserineCombined sources | 1 | |
Modified residuei | 290 | PhosphoserineBy similarity | 1 | |
Modified residuei | 394 | PhosphoserineBy similarity | 1 | |
Modified residuei | 407 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1076 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1563 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1566 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1 (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | G3X9K3. |
MaxQBi | G3X9K3. |
PaxDbi | G3X9K3. |
PeptideAtlasi | G3X9K3. |
PRIDEi | G3X9K3. |
PTM databases
iPTMneti | G3X9K3. |
PhosphoSitePlusi | G3X9K3. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000067851. |
ExpressionAtlasi | G3X9K3. baseline and differential. |
Genevisiblei | G3X9K3. MM. |
Interactioni
Subunit structurei
Homodimer. Interacts with ARFGEF2/BIG2; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2. Interacts with MYO9B. Interacts with PRKAR1A and PRKAR2A. Interacts with PPP1CC. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA. Interacts with DPY30. Interacts with PDE3A. Interacts with KANK1. Interacts with TBC1D22A and TBC1D22B.By similarity
GO - Molecular functioni
- ARF guanyl-nucleotide exchange factor activity Source: GO_Central
- guanyl-nucleotide exchange factor activity Source: MGI
- myosin binding Source: MGI
- protein kinase A regulatory subunit binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000085986. |
Structurei
3D structure databases
ProteinModelPortali | G3X9K3. |
SMRi | G3X9K3. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 688 – 877 | SEC7PROSITE-ProRule annotationAdd BLAST | 190 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 224 | DCB; DCB:DCB domain and DCB:HUS domain interactionBy similarityAdd BLAST | 223 | |
Regioni | 554 – 574 | HUS; DCB:HUS domain interactionBy similarityAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 708 – 712 | Nuclear localization signal (NLS)By similarity | 5 |
Phylogenomic databases
eggNOGi | KOG0929. Eukaryota. COG5307. LUCA. |
GeneTreei | ENSGT00760000119036. |
HOGENOMi | HOG000181045. |
InParanoidi | G3X9K3. |
KOi | K18442. |
OMAi | LLWNMEM. |
OrthoDBi | EOG091G00F0. |
TreeFami | TF300714. |
Family and domain databases
Gene3Di | 1.10.1000.11. 1 hit. |
InterProi | View protein in InterPro IPR016024. ARM-type_fold. IPR032629. DCB_dom. IPR015403. Sec7_C. IPR023394. Sec7_C_sf. IPR000904. Sec7_dom. IPR035999. Sec7_dom_sf. IPR032691. Sec7_N. |
Pfami | View protein in Pfam PF16213. DCB. 1 hit. PF09324. DUF1981. 1 hit. PF01369. Sec7. 1 hit. PF12783. Sec7_N. 1 hit. |
SMARTi | View protein in SMART SM00222. Sec7. 1 hit. |
SUPFAMi | SSF48371. SSF48371. 3 hits. SSF48425. SSF48425. 1 hit. |
PROSITEi | View protein in PROSITE PS50190. SEC7. 1 hit. |
i Sequence
Sequence statusi: Complete.
G3X9K3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKVETEK
60 70 80 90 100
QSPPHGEAKA GSGTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL
110 120 130 140 150
DCLQKLIAYG HLTGRAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ
160 170 180 190 200
IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ
210 220 230 240 250
MLNVIFARME NQALQEAKQM ERERHRQQQH LLQSPVSHHE PESPHLRYLP
260 270 280 290 300
PQTVDHINQE HEGDLEPQTH DVDKSLQDDT EPENGSDISS AENEQTEADQ
310 320 330 340 350
ATAAETLSKN DILYDGDYEE KPLDIVQSIV EEMVNIIVGD MGEGMAISAS
360 370 380 390 400
TEGNTGTVED GSDSENIQAN GIPGTPISVA YTPSLPDDRL SVSSNDTQES
410 420 430 440 450
GNSSGPSPGA KFSHILQKDA FLVFRSLCKL SMKPLSDGPP DPKSHELRSK
460 470 480 490 500
ILSLQLLLSI LQNAGPVFRT NEMFINAIKQ YLCVALSKNG VSSVPEVFEL
510 520 530 540 550
SLSIFLTLLS NFKTHLKMQI EVFFKEIFLY ILETSTSSFD HKWMVIQTLT
560 570 580 590 600
RICADAQSVV DIYVNYDCDL NAANIFERLV NDLSKIAQGR GSQELGMSNV
610 620 630 640 650
QELSLRKKGL ECLVSILKCM VEWSKDQYVN PNSQTTLGQE KPSEQEISEV
660 670 680 690 700
KHPETINRYG SLNSLESTSS SGIGSYSTQM SGTDNPEQFE VLKQQKEIIE
710 720 730 740 750
QGIDLFNKKP KRGIQYLQEQ GMLGTTPEDI AQFLHQEERL DSTQVGEFLG
760 770 780 790 800
DNDKFNKEVM YAYVDQHDFS GKDFVSALRL FLEGFRLPGE AQKIDRLMEK
810 820 830 840 850
FAARYLECNQ GQTLFASADT AYVLAYSIIM LTTDLHSPQV KNKMTKEQYI
860 870 880 890 900
KMNRGINDSK DLPEEYLSAI YNEIAGKKIS MKETKELTIP TKSTKQNVAS
910 920 930 940 950
EKQRRLLYNL EMEQMAKTAK ALMEAVSHVQ APFTSATHLE HVRPMFKLAW
960 970 980 990 1000
TPFLAAFSVG LQDCDDTEVA SLCLEGIRCA IRIACIFSIQ LERDAYVQAL
1010 1020 1030 1040 1050
ARFTLLTVSS GITEMKQKNI DTIKTLITVA HTDGNYLGNS WHEILKCISQ
1060 1070 1080 1090 1100
LELAQLIGTG VKPRYISGTV RGREGSLTGT KDQAPDEFVG LGLVGGNVDW
1110 1120 1130 1140 1150
KQIASIQESI GETSSQSVVV AVDRIFTGST RLDGNAIVDF VRWLCAVSMD
1160 1170 1180 1190 1200
ELLSTTHPRM FSLQKIVEIS YYNMGRIRLQ WSRIWEVIGD HFNKVGCNPN
1210 1220 1230 1240 1250
EDVAIFAVDS LRQLSMKFLE KGELANFRFQ KDFLRPFEHI MKRNRSPTIR
1260 1270 1280 1290 1300
DMVVRCIAQM VNSQAANIRS GWKNIFSVFH LAASDQDESI VELAFQTTGH
1310 1320 1330 1340 1350
IVTLVFEKHF PATIDSFQDA VKCLSEFACN AAFPDTSMEA IRLIRHCAKY
1360 1370 1380 1390 1400
VSDRPQAFKE YTSDDMSVAP EDRVWVRGWF PILFELSCII NRCKLDVRTR
1410 1420 1430 1440 1450
GLTVMFEIMK TYGHTYEKHW WQDLFRIVFR IFDNMKLPEQ QTEKAEWMTT
1460 1470 1480 1490 1500
TCNHALYAIC DVFTQYLEVL SDVLLDDIFA QLYWCVQQDN EQLARSGTNC
1510 1520 1530 1540 1550
LENVVILNGE KFTLEIWDKT CNCTLDIFKT TIPHALLTWR PTSGEAEPPS
1560 1570 1580 1590 1600
PSAVSEKPLD AISQKSVDIH DSIQPRSSDN RQQAPLVSVS TVSEEVSKVK
1610 1620 1630 1640 1650
STAKFPEQKL FAALLIKCVV QLELIQTIDN IVFFPATSKK EDAENLAAAQ
1660 1670 1680 1690 1700
RDAVDFDVRV DTQDQGMYRF LTSQQLFKLL DCLLESHRFA KAFNSNNEQR
1710 1720 1730 1740 1750
TALWKAGFKG KSKPNLLKQE TSSLACGLRI LFRMYMDESR VSAWEEVQQR
1760 1770 1780 1790 1800
LLNVCREALS YFLTLTSESH REAWTNLLLL FLTKVLKISD SRFKAHASFY
1810 1820 1830 1840
YPLLCEIMQF DLIPELRAVL RRFFLRIGIV FQISQPPEQE LGINRQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC102462 Genomic DNA. No translation available. AC159972 Genomic DNA. No translation available. CH466536 Genomic DNA. Translation: EDL14306.1. AK051592 mRNA. Translation: BAC34685.1. |
CCDSi | CCDS35512.1. |
RefSeqi | NP_001095900.1. NM_001102430.1. |
UniGenei | Mm.229141. |
Genome annotation databases
Ensembli | ENSMUST00000088615; ENSMUSP00000085986; ENSMUSG00000067851. |
GeneIDi | 211673. |
KEGGi | mmu:211673. |
UCSCi | uc007ahn.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | BIG1_MOUSE | |
Accessioni | G3X9K3Primary (citable) accession number: G3X9K3 Secondary accession number(s): Q8BKL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | November 16, 2011 | |
Last modified: | February 28, 2018 | |
This is version 53 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |