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UniProtKB - G3X9K3 (BIG1_MOUSE)

UniProt

G3X9K3 - BIG1_MOUSE

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Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Gene

Arfgef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).By similarity

Enzyme regulationi

Inhibited by brefeldin A.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Short name:
BIG1
Short name:
Brefeldin A-inhibited GEP 1
Alternative name(s):
ADP-ribosylation factor guanine nucleotide-exchange factor 1
Gene namesi
Name:Arfgef1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442988. Arfgef1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18461846Brefeldin A-inhibited guanine nucleotide-exchange protein 1PRO_0000419330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei234 – 2341PhosphoserineBy similarity
Modified residuei243 – 2431PhosphoserineBy similarity
Modified residuei269 – 2691PhosphothreonineBy similarity
Modified residuei286 – 2861PhosphoserineCombined sources
Modified residuei289 – 2891PhosphoserineCombined sources
Modified residuei290 – 2901PhosphoserineBy similarity
Modified residuei393 – 3931PhosphoserineBy similarity
Modified residuei394 – 3941PhosphoserineBy similarity
Modified residuei407 – 4071PhosphoserineBy similarity
Modified residuei661 – 6611PhosphoserineBy similarity
Modified residuei664 – 6641PhosphoserineBy similarity
Modified residuei678 – 6781PhosphothreonineBy similarity
Modified residuei1076 – 10761PhosphoserineBy similarity
Modified residuei1563 – 15631PhosphoserineBy similarity
Modified residuei1566 – 15661PhosphoserineBy similarity
Modified residuei1590 – 15901PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiG3X9K3.
MaxQBiG3X9K3.
PaxDbiG3X9K3.
PeptideAtlasiG3X9K3.
PRIDEiG3X9K3.

PTM databases

iPTMnetiG3X9K3.
PhosphoSiteiG3X9K3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000067851.
ExpressionAtlasiG3X9K3. baseline and differential.
GenevisibleiG3X9K3. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with ARFGEF2/BIG2; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2. Interacts with MYO9B. Interacts with PRKAR1A and PRKAR2A. Interacts with PPP1CC. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA. Interacts with DPY30. Interacts with PDE3A. Interacts with KANK1. Interacts with TBC1D22A and TBC1D22B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000085986.

Structurei

3D structure databases

ProteinModelPortaliG3X9K3.
SMRiG3X9K3. Positions 692-882.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini688 – 877190SEC7PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 224223DCB; DCB:DCB domain and DCB:HUS domain interactionBy similarityAdd
BLAST
Regioni554 – 57421HUS; DCB:HUS domain interactionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi708 – 7125Nuclear localization signal (NLS)By similarity

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000181045.
InParanoidiG3X9K3.
KOiK18442.
OMAiHEPESPH.
OrthoDBiEOG091G00F0.
TreeFamiTF300714.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G3X9K3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKVETEK 
        60         70         80         90        100
QSPPHGEAKA GSGTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL 
       110        120        130        140        150
DCLQKLIAYG HLTGRAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ 
       160        170        180        190        200
IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ 
       210        220        230        240        250
MLNVIFARME NQALQEAKQM ERERHRQQQH LLQSPVSHHE PESPHLRYLP 
       260        270        280        290        300
PQTVDHINQE HEGDLEPQTH DVDKSLQDDT EPENGSDISS AENEQTEADQ 
       310        320        330        340        350
ATAAETLSKN DILYDGDYEE KPLDIVQSIV EEMVNIIVGD MGEGMAISAS 
       360        370        380        390        400
TEGNTGTVED GSDSENIQAN GIPGTPISVA YTPSLPDDRL SVSSNDTQES 
       410        420        430        440        450
GNSSGPSPGA KFSHILQKDA FLVFRSLCKL SMKPLSDGPP DPKSHELRSK 
       460        470        480        490        500
ILSLQLLLSI LQNAGPVFRT NEMFINAIKQ YLCVALSKNG VSSVPEVFEL 
       510        520        530        540        550
SLSIFLTLLS NFKTHLKMQI EVFFKEIFLY ILETSTSSFD HKWMVIQTLT 
       560        570        580        590        600
RICADAQSVV DIYVNYDCDL NAANIFERLV NDLSKIAQGR GSQELGMSNV 
       610        620        630        640        650
QELSLRKKGL ECLVSILKCM VEWSKDQYVN PNSQTTLGQE KPSEQEISEV 
       660        670        680        690        700
KHPETINRYG SLNSLESTSS SGIGSYSTQM SGTDNPEQFE VLKQQKEIIE 
       710        720        730        740        750
QGIDLFNKKP KRGIQYLQEQ GMLGTTPEDI AQFLHQEERL DSTQVGEFLG 
       760        770        780        790        800
DNDKFNKEVM YAYVDQHDFS GKDFVSALRL FLEGFRLPGE AQKIDRLMEK 
       810        820        830        840        850
FAARYLECNQ GQTLFASADT AYVLAYSIIM LTTDLHSPQV KNKMTKEQYI 
       860        870        880        890        900
KMNRGINDSK DLPEEYLSAI YNEIAGKKIS MKETKELTIP TKSTKQNVAS 
       910        920        930        940        950
EKQRRLLYNL EMEQMAKTAK ALMEAVSHVQ APFTSATHLE HVRPMFKLAW 
       960        970        980        990       1000
TPFLAAFSVG LQDCDDTEVA SLCLEGIRCA IRIACIFSIQ LERDAYVQAL 
      1010       1020       1030       1040       1050
ARFTLLTVSS GITEMKQKNI DTIKTLITVA HTDGNYLGNS WHEILKCISQ 
      1060       1070       1080       1090       1100
LELAQLIGTG VKPRYISGTV RGREGSLTGT KDQAPDEFVG LGLVGGNVDW 
      1110       1120       1130       1140       1150
KQIASIQESI GETSSQSVVV AVDRIFTGST RLDGNAIVDF VRWLCAVSMD 
      1160       1170       1180       1190       1200
ELLSTTHPRM FSLQKIVEIS YYNMGRIRLQ WSRIWEVIGD HFNKVGCNPN 
      1210       1220       1230       1240       1250
EDVAIFAVDS LRQLSMKFLE KGELANFRFQ KDFLRPFEHI MKRNRSPTIR 
      1260       1270       1280       1290       1300
DMVVRCIAQM VNSQAANIRS GWKNIFSVFH LAASDQDESI VELAFQTTGH 
      1310       1320       1330       1340       1350
IVTLVFEKHF PATIDSFQDA VKCLSEFACN AAFPDTSMEA IRLIRHCAKY 
      1360       1370       1380       1390       1400
VSDRPQAFKE YTSDDMSVAP EDRVWVRGWF PILFELSCII NRCKLDVRTR 
      1410       1420       1430       1440       1450
GLTVMFEIMK TYGHTYEKHW WQDLFRIVFR IFDNMKLPEQ QTEKAEWMTT 
      1460       1470       1480       1490       1500
TCNHALYAIC DVFTQYLEVL SDVLLDDIFA QLYWCVQQDN EQLARSGTNC 
      1510       1520       1530       1540       1550
LENVVILNGE KFTLEIWDKT CNCTLDIFKT TIPHALLTWR PTSGEAEPPS 
      1560       1570       1580       1590       1600
PSAVSEKPLD AISQKSVDIH DSIQPRSSDN RQQAPLVSVS TVSEEVSKVK 
      1610       1620       1630       1640       1650
STAKFPEQKL FAALLIKCVV QLELIQTIDN IVFFPATSKK EDAENLAAAQ 
      1660       1670       1680       1690       1700
RDAVDFDVRV DTQDQGMYRF LTSQQLFKLL DCLLESHRFA KAFNSNNEQR 
      1710       1720       1730       1740       1750
TALWKAGFKG KSKPNLLKQE TSSLACGLRI LFRMYMDESR VSAWEEVQQR 
      1760       1770       1780       1790       1800
LLNVCREALS YFLTLTSESH REAWTNLLLL FLTKVLKISD SRFKAHASFY 
      1810       1820       1830       1840 
YPLLCEIMQF DLIPELRAVL RRFFLRIGIV FQISQPPEQE LGINRQ
Length:1,846
Mass (Da):208,500
Last modified:November 16, 2011 - v1
Checksum:i7108F4430E610CB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102462 Genomic DNA. No translation available.
AC159972 Genomic DNA. No translation available.
CH466536 Genomic DNA. Translation: EDL14306.1.
AK051592 mRNA. Translation: BAC34685.1.
CCDSiCCDS35512.1.
RefSeqiNP_001095900.1. NM_001102430.1.
UniGeneiMm.229141.

Genome annotation databases

EnsembliENSMUST00000088615; ENSMUSP00000085986; ENSMUSG00000067851.
GeneIDi211673.
KEGGimmu:211673.
UCSCiuc007ahn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102462 Genomic DNA. No translation available.
AC159972 Genomic DNA. No translation available.
CH466536 Genomic DNA. Translation: EDL14306.1.
AK051592 mRNA. Translation: BAC34685.1.
CCDSiCCDS35512.1.
RefSeqiNP_001095900.1. NM_001102430.1.
UniGeneiMm.229141.

3D structure databases

ProteinModelPortaliG3X9K3.
SMRiG3X9K3. Positions 692-882.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000085986.

PTM databases

iPTMnetiG3X9K3.
PhosphoSiteiG3X9K3.

Proteomic databases

EPDiG3X9K3.
MaxQBiG3X9K3.
PaxDbiG3X9K3.
PeptideAtlasiG3X9K3.
PRIDEiG3X9K3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000088615; ENSMUSP00000085986; ENSMUSG00000067851.
GeneIDi211673.
KEGGimmu:211673.
UCSCiuc007ahn.1. mouse.

Organism-specific databases

CTDi10565.
MGIiMGI:2442988. Arfgef1.

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000181045.
InParanoidiG3X9K3.
KOiK18442.
OMAiHEPESPH.
OrthoDBiEOG091G00F0.
TreeFamiTF300714.

Miscellaneous databases

ChiTaRSiArfgef1. mouse.
PROiG3X9K3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067851.
ExpressionAtlasiG3X9K3. baseline and differential.
GenevisibleiG3X9K3. MM.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIG1_MOUSE
AccessioniPrimary (citable) accession number: G3X9K3
Secondary accession number(s): Q8BKL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: November 16, 2011
Last modified: September 7, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.