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Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Gene

Arfgef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).By similarity

Enzyme regulationi

Inhibited by brefeldin A.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Short name:
BIG1
Short name:
Brefeldin A-inhibited GEP 1
Alternative name(s):
ADP-ribosylation factor guanine nucleotide-exchange factor 1
Gene namesi
Name:Arfgef1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442988. Arfgef1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18461846Brefeldin A-inhibited guanine nucleotide-exchange protein 1PRO_0000419330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei234 – 2341PhosphoserineBy similarity
Modified residuei243 – 2431PhosphoserineBy similarity
Modified residuei286 – 2861PhosphoserineCombined sources
Modified residuei289 – 2891PhosphoserineCombined sources
Modified residuei290 – 2901PhosphoserineBy similarity
Modified residuei393 – 3931PhosphoserineBy similarity
Modified residuei394 – 3941PhosphoserineBy similarity
Modified residuei661 – 6611PhosphoserineBy similarity
Modified residuei664 – 6641PhosphoserineBy similarity
Modified residuei678 – 6781PhosphothreonineBy similarity
Modified residuei1076 – 10761PhosphoserineBy similarity
Modified residuei1566 – 15661PhosphoserineBy similarity
Modified residuei1590 – 15901PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiG3X9K3.
MaxQBiG3X9K3.
PaxDbiG3X9K3.
PRIDEiG3X9K3.

PTM databases

iPTMnetiG3X9K3.

Expressioni

Gene expression databases

BgeeiG3X9K3.
ExpressionAtlasiG3X9K3. baseline and differential.
GenevisibleiG3X9K3. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2, DPY30, MYO9B, PRKAR1A, PRKAR2A, PPP1CC and PDE3A. Interacts with KANK1; however, colocalization cannot be experimentally confirmed. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000085986.

Structurei

3D structure databases

ProteinModelPortaliG3X9K3.
SMRiG3X9K3. Positions 692-882.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini688 – 877190SEC7PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 224223DCB; DCB:DCB domain and DCB:HUS domain interactionBy similarityAdd
BLAST
Regioni554 – 57421HUS; DCB:HUS domain interactionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi708 – 7125Nuclear localization signal (NLS)By similarity

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000181045.
InParanoidiG3X9K3.
KOiK18442.
OMAiHEPESPH.
OrthoDBiEOG7QVM1S.
TreeFamiTF300714.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G3X9K3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKVETEK
60 70 80 90 100
QSPPHGEAKA GSGTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL
110 120 130 140 150
DCLQKLIAYG HLTGRAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ
160 170 180 190 200
IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ
210 220 230 240 250
MLNVIFARME NQALQEAKQM ERERHRQQQH LLQSPVSHHE PESPHLRYLP
260 270 280 290 300
PQTVDHINQE HEGDLEPQTH DVDKSLQDDT EPENGSDISS AENEQTEADQ
310 320 330 340 350
ATAAETLSKN DILYDGDYEE KPLDIVQSIV EEMVNIIVGD MGEGMAISAS
360 370 380 390 400
TEGNTGTVED GSDSENIQAN GIPGTPISVA YTPSLPDDRL SVSSNDTQES
410 420 430 440 450
GNSSGPSPGA KFSHILQKDA FLVFRSLCKL SMKPLSDGPP DPKSHELRSK
460 470 480 490 500
ILSLQLLLSI LQNAGPVFRT NEMFINAIKQ YLCVALSKNG VSSVPEVFEL
510 520 530 540 550
SLSIFLTLLS NFKTHLKMQI EVFFKEIFLY ILETSTSSFD HKWMVIQTLT
560 570 580 590 600
RICADAQSVV DIYVNYDCDL NAANIFERLV NDLSKIAQGR GSQELGMSNV
610 620 630 640 650
QELSLRKKGL ECLVSILKCM VEWSKDQYVN PNSQTTLGQE KPSEQEISEV
660 670 680 690 700
KHPETINRYG SLNSLESTSS SGIGSYSTQM SGTDNPEQFE VLKQQKEIIE
710 720 730 740 750
QGIDLFNKKP KRGIQYLQEQ GMLGTTPEDI AQFLHQEERL DSTQVGEFLG
760 770 780 790 800
DNDKFNKEVM YAYVDQHDFS GKDFVSALRL FLEGFRLPGE AQKIDRLMEK
810 820 830 840 850
FAARYLECNQ GQTLFASADT AYVLAYSIIM LTTDLHSPQV KNKMTKEQYI
860 870 880 890 900
KMNRGINDSK DLPEEYLSAI YNEIAGKKIS MKETKELTIP TKSTKQNVAS
910 920 930 940 950
EKQRRLLYNL EMEQMAKTAK ALMEAVSHVQ APFTSATHLE HVRPMFKLAW
960 970 980 990 1000
TPFLAAFSVG LQDCDDTEVA SLCLEGIRCA IRIACIFSIQ LERDAYVQAL
1010 1020 1030 1040 1050
ARFTLLTVSS GITEMKQKNI DTIKTLITVA HTDGNYLGNS WHEILKCISQ
1060 1070 1080 1090 1100
LELAQLIGTG VKPRYISGTV RGREGSLTGT KDQAPDEFVG LGLVGGNVDW
1110 1120 1130 1140 1150
KQIASIQESI GETSSQSVVV AVDRIFTGST RLDGNAIVDF VRWLCAVSMD
1160 1170 1180 1190 1200
ELLSTTHPRM FSLQKIVEIS YYNMGRIRLQ WSRIWEVIGD HFNKVGCNPN
1210 1220 1230 1240 1250
EDVAIFAVDS LRQLSMKFLE KGELANFRFQ KDFLRPFEHI MKRNRSPTIR
1260 1270 1280 1290 1300
DMVVRCIAQM VNSQAANIRS GWKNIFSVFH LAASDQDESI VELAFQTTGH
1310 1320 1330 1340 1350
IVTLVFEKHF PATIDSFQDA VKCLSEFACN AAFPDTSMEA IRLIRHCAKY
1360 1370 1380 1390 1400
VSDRPQAFKE YTSDDMSVAP EDRVWVRGWF PILFELSCII NRCKLDVRTR
1410 1420 1430 1440 1450
GLTVMFEIMK TYGHTYEKHW WQDLFRIVFR IFDNMKLPEQ QTEKAEWMTT
1460 1470 1480 1490 1500
TCNHALYAIC DVFTQYLEVL SDVLLDDIFA QLYWCVQQDN EQLARSGTNC
1510 1520 1530 1540 1550
LENVVILNGE KFTLEIWDKT CNCTLDIFKT TIPHALLTWR PTSGEAEPPS
1560 1570 1580 1590 1600
PSAVSEKPLD AISQKSVDIH DSIQPRSSDN RQQAPLVSVS TVSEEVSKVK
1610 1620 1630 1640 1650
STAKFPEQKL FAALLIKCVV QLELIQTIDN IVFFPATSKK EDAENLAAAQ
1660 1670 1680 1690 1700
RDAVDFDVRV DTQDQGMYRF LTSQQLFKLL DCLLESHRFA KAFNSNNEQR
1710 1720 1730 1740 1750
TALWKAGFKG KSKPNLLKQE TSSLACGLRI LFRMYMDESR VSAWEEVQQR
1760 1770 1780 1790 1800
LLNVCREALS YFLTLTSESH REAWTNLLLL FLTKVLKISD SRFKAHASFY
1810 1820 1830 1840
YPLLCEIMQF DLIPELRAVL RRFFLRIGIV FQISQPPEQE LGINRQ
Length:1,846
Mass (Da):208,500
Last modified:November 16, 2011 - v1
Checksum:i7108F4430E610CB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102462 Genomic DNA. No translation available.
AC159972 Genomic DNA. No translation available.
CH466536 Genomic DNA. Translation: EDL14306.1.
AK051592 mRNA. Translation: BAC34685.1.
CCDSiCCDS35512.1.
RefSeqiNP_001095900.1. NM_001102430.1.
UniGeneiMm.229141.

Genome annotation databases

EnsembliENSMUST00000088615; ENSMUSP00000085986; ENSMUSG00000067851.
GeneIDi211673.
KEGGimmu:211673.
UCSCiuc007ahn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102462 Genomic DNA. No translation available.
AC159972 Genomic DNA. No translation available.
CH466536 Genomic DNA. Translation: EDL14306.1.
AK051592 mRNA. Translation: BAC34685.1.
CCDSiCCDS35512.1.
RefSeqiNP_001095900.1. NM_001102430.1.
UniGeneiMm.229141.

3D structure databases

ProteinModelPortaliG3X9K3.
SMRiG3X9K3. Positions 692-882.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000085986.

PTM databases

iPTMnetiG3X9K3.

Proteomic databases

EPDiG3X9K3.
MaxQBiG3X9K3.
PaxDbiG3X9K3.
PRIDEiG3X9K3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000088615; ENSMUSP00000085986; ENSMUSG00000067851.
GeneIDi211673.
KEGGimmu:211673.
UCSCiuc007ahn.1. mouse.

Organism-specific databases

CTDi10565.
MGIiMGI:2442988. Arfgef1.

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000181045.
InParanoidiG3X9K3.
KOiK18442.
OMAiHEPESPH.
OrthoDBiEOG7QVM1S.
TreeFamiTF300714.

Miscellaneous databases

ChiTaRSiArfgef1. mouse.
NextBioi373332.
PROiG3X9K3.
SOURCEiSearch...

Gene expression databases

BgeeiG3X9K3.
ExpressionAtlasiG3X9K3. baseline and differential.
GenevisibleiG3X9K3. MM.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1441-1846.
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiBIG1_MOUSE
AccessioniPrimary (citable) accession number: G3X9K3
Secondary accession number(s): Q8BKL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: November 16, 2011
Last modified: May 11, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.