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Protein

Sodium/hydrogen exchanger 3

Gene

Slc9a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.By similarity

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB
  • sodium:proton antiporter activity Source: MGI

GO - Biological processi

  • regulation of pH Source: MGI
  • sodium ion import across plasma membrane Source: UniProtKB
  • sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-MMU-425986. Sodium/Proton exchangers.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 3Curated
Gene namesi
Name:Slc9a3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:105064. Slc9a3.

Subcellular locationi

  • Apical cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: In intestinal epithelial cells, localizes to the ileal brush border. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Angiotensin-2 enhances apical expression (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicCuratedAdd BLAST11
Intramembranei12 – 23Name=A/M1Sequence analysisAdd BLAST12
Topological domaini24 – 49CytoplasmicCuratedAdd BLAST26
Intramembranei50 – 68Name=B/M2Sequence analysisAdd BLAST19
Topological domaini69 – 74CytoplasmicCurated6
Transmembranei75 – 94Helical; Name=C/M3Sequence analysisAdd BLAST20
Topological domaini95 – 107ExtracellularCuratedAdd BLAST13
Transmembranei108 – 128Helical; Name=D/M4Sequence analysisAdd BLAST21
Topological domaini129 – 134CytoplasmicCurated6
Transmembranei135 – 155Helical; Name=E/M5Sequence analysisAdd BLAST21
Topological domaini156 – 175ExtracellularCuratedAdd BLAST20
Transmembranei176 – 197Helical; Name=F/M5ASequence analysisAdd BLAST22
Topological domaini198 – 205CytoplasmicCurated8
Transmembranei206 – 227Helical; Name=G/M5BSequence analysisAdd BLAST22
Topological domaini228 – 247ExtracellularCuratedAdd BLAST20
Transmembranei248 – 269Helical; Name=H/M6Sequence analysisAdd BLAST22
Topological domaini270 – 285CytoplasmicCuratedAdd BLAST16
Transmembranei286 – 304Helical; Name=I/M7Sequence analysisAdd BLAST19
Topological domaini305 – 335ExtracellularCuratedAdd BLAST31
Transmembranei336 – 357Helical; Name=J/M8Sequence analysisAdd BLAST22
Topological domaini358 – 364CytoplasmicCurated7
Transmembranei365 – 385Helical; Name=K/M9Sequence analysisAdd BLAST21
Topological domaini386 – 400ExtracellularCuratedAdd BLAST15
Intramembranei401 – 421Name=LSequence analysisAdd BLAST21
Topological domaini422 – 430ExtracellularCurated9
Transmembranei431 – 451Helical; Name=M/M10Sequence analysisAdd BLAST21
Topological domaini452 – 829CytoplasmicCuratedAdd BLAST378

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • brush border membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane-bounded vesicle Source: MGI
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004333551 – 829Sodium/hydrogen exchanger 3Add BLAST829

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Modified residuei550PhosphoserineBy similarity1
Modified residuei558PhosphoserineBy similarity1
Modified residuei588PhosphoserineCombined sources1
Modified residuei603PhosphoserineBy similarity1
Modified residuei659Phosphoserine; by SGK1By similarity1
Modified residuei805PhosphoserineCombined sources1
Modified residuei808PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PKA, which inhibits activity. Phosphorylation at Ser-659 by SGK1 is associated with increased abundance at the cell membrane.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiG3X939.
PaxDbiG3X939.
PeptideAtlasiG3X939.

PTM databases

iPTMnetiG3X939.
PhosphoSitePlusiG3X939.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036123.

Interactioni

Subunit structurei

Binds SLC9A3R1 and SLC9A3R2. Interacts with CHP1, CHP2 and SHANK2. Interacts with PDZK1 (via C-terminal PDZ domain) (By similarity). Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Interacts with AHCYL1; the interaction is required for SLC9A3 activity (By similarity).By similarity

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038142.

Structurei

3D structure databases

ProteinModelPortaliG3X939.
SMRiG3X939.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni586 – 663Interaction with PDZD3By similarityAdd BLAST78

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
KOiK12040.
OMAiYTMKMLA.
OrthoDBiEOG091G02Q0.
TreeFamiTF317212.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018410. Na/H_exchanger_3/5.
IPR004709. NaH_exchanger.
IPR011256. Reg_factor_effector_dom.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 2 hits.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01087. NAHEXCHNGR3.
SUPFAMiSSF55136. SSF55136. 1 hit.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

G3X939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWHRALGPGW KLLLALALTS LQGARGAEEE PSSDGSFQVV TFKWHHVQDP
60 70 80 90 100
YIIALWILVA SLAKIVFHLS HKVTSIVPES ALLIVLGLVL GGIVWAADHI
110 120 130 140 150
ASFTLTPTLF FFYLLPPIVL DAGYFMPNRL FFGNLGTILL YAVIGTIWNA
160 170 180 190 200
ATTGLSLYGV FLSGLMGELK IGLLDFLLFG SLIAAVDPVA VLAVFEEVHV
210 220 230 240 250
NEVLFIIVFG ESLLNDAVTV VLYNVFESFV TLGGDAVTGV DCVKGIVSFF
260 270 280 290 300
VVSLGGTLVG VIFAFLLSLV TRFTKHVRII EPGFVFVISY LSYLTSEMLS
310 320 330 340 350
LSSILAITFC GICCQKYVKA NISEQSATTV RYTMKMLASG AETIIFMFLG
360 370 380 390 400
ISAVNPDIWT WNTAFVLLTL VFISVYRAIG VVLQTWILNR YRMVQLETID
410 420 430 440 450
QVVMSYGGLR GAVAYALVVL LDEKKVKEKN LFVSTTLIVV FFTVIFQGLT
460 470 480 490 500
IKPLVQWLKV KRSEHREPKL NEKLHGRAFD HILSAIEDIS GQIGHNYLRD
510 520 530 540 550
KWSNFDRKFL SKVLMRRSAQ KSRDRILNVF HELNLKDAIS YVAEGERRGS
560 570 580 590 600
LAFIRSPSTD NMVNVDFNTP RPSTVEASVS YFLRENVSAV CLDMQSLEQR
610 620 630 640 650
RRSIRDTEDM VTHHTLQQYL YKPRQEYKHL YSRHELTPNE DEKQDKEIFH
660 670 680 690 700
RTMRKRLESF KSAKLGINQN KKAAKLYKRE RAQKRRNSSI PNGKLPMENL
710 720 730 740 750
AHNYTIKEKD LELSEHEEAT NYEEISGGIE FLASVTQDVA SDSGAGIDNP
760 770 780 790 800
VFSPDEDLDP SILSRVPPWL SPGETVVPSQ RARVQIPNSP SNFRRLTPFR
810 820
LSNKSVDSFL QADGHEEQLQ PAAPESTHM
Length:829
Mass (Da):93,104
Last modified:November 16, 2011 - v1
Checksum:iE2458CAE3F11B357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033564 mRNA. Translation: BAC28362.1.
AC154839 Genomic DNA. No translation available.
CH466563 Genomic DNA. Translation: EDL37085.1.
CCDSiCCDS36731.1.
RefSeqiNP_001074529.1. NM_001081060.1.
XP_006517085.1. XM_006517022.3.
UniGeneiMm.261564.

Genome annotation databases

EnsembliENSMUST00000036208; ENSMUSP00000038142; ENSMUSG00000036123.
GeneIDi105243.
KEGGimmu:105243.
UCSCiuc007res.2. mouse.
uc007ret.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033564 mRNA. Translation: BAC28362.1.
AC154839 Genomic DNA. No translation available.
CH466563 Genomic DNA. Translation: EDL37085.1.
CCDSiCCDS36731.1.
RefSeqiNP_001074529.1. NM_001081060.1.
XP_006517085.1. XM_006517022.3.
UniGeneiMm.261564.

3D structure databases

ProteinModelPortaliG3X939.
SMRiG3X939.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038142.

PTM databases

iPTMnetiG3X939.
PhosphoSitePlusiG3X939.

Proteomic databases

MaxQBiG3X939.
PaxDbiG3X939.
PeptideAtlasiG3X939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036208; ENSMUSP00000038142; ENSMUSG00000036123.
GeneIDi105243.
KEGGimmu:105243.
UCSCiuc007res.2. mouse.
uc007ret.1. mouse.

Organism-specific databases

CTDi6550.
MGIiMGI:105064. Slc9a3.

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
KOiK12040.
OMAiYTMKMLA.
OrthoDBiEOG091G02Q0.
TreeFamiTF317212.

Enzyme and pathway databases

ReactomeiR-MMU-425986. Sodium/Proton exchangers.

Miscellaneous databases

PROiG3X939.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036123.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018410. Na/H_exchanger_3/5.
IPR004709. NaH_exchanger.
IPR011256. Reg_factor_effector_dom.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 2 hits.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01087. NAHEXCHNGR3.
SUPFAMiSSF55136. SSF55136. 1 hit.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSL9A3_MOUSE
AccessioniPrimary (citable) accession number: G3X939
Secondary accession number(s): Q8BZU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: November 16, 2011
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.