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Protein

Prothrombin

Gene

F2

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.UniRule annotation

Catalytic activityi

Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei402Charge relay systemUniRule annotation1
Active sitei458Charge relay systemUniRule annotation1
Active sitei564Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine proteaseUniRule annotationSAAS annotation
Biological processAcute phaseUniRule annotation, Blood coagulationUniRule annotation, Hemostasis
LigandCalciumSAAS annotation

Enzyme and pathway databases

ReactomeiR-RNO-140837. Intrinsic Pathway of Fibrin Clot Formation.
R-RNO-140875. Common Pathway of Fibrin Clot Formation.
R-RNO-159740. Gamma-carboxylation of protein precursors.
R-RNO-159763. Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus.
R-RNO-159782. Removal of aminoterminal propeptides from gamma-carboxylated proteins.
R-RNO-202733. Cell surface interactions at the vascular wall.
R-RNO-375276. Peptide ligand-binding receptors.
R-RNO-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-RNO-416476. G alpha (q) signalling events.
R-RNO-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-RNO-76009. Platelet Aggregation (Plug Formation).
R-RNO-977606. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
ProthrombinUniRule annotation (EC:3.4.21.5UniRule annotation)
Alternative name(s):
Coagulation factor IIUniRule annotation
Gene namesi
Name:F2Imported
ORF Names:rCG_27251Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi61996. F2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_501120664525 – 617ProthrombinSequence analysisAdd BLAST593

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi61 ↔ 66UniRule annotation
Disulfide bondi91 ↔ 104UniRule annotation
Disulfide bondi109 ↔ 187UniRule annotation
Disulfide bondi130 ↔ 170UniRule annotation
Disulfide bondi158 ↔ 182UniRule annotation
Disulfide bondi215 ↔ 292UniRule annotation
Disulfide bondi236 ↔ 276UniRule annotation
Disulfide bondi264 ↔ 287UniRule annotation
Disulfide bondi332 ↔ 478Interchain (between light and heavy chains)UniRule annotation
Disulfide bondi387 ↔ 403UniRule annotation
Disulfide bondi532 ↔ 546UniRule annotation
Disulfide bondi560 ↔ 590UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei200 – 201Cleavage; by thrombinUniRule annotation2
Sitei323 – 324Cleavage; by factor XaUniRule annotation2
Sitei359 – 360Cleavage; by factor XaUniRule annotation2

Keywords - PTMi

Disulfide bondUniRule annotationSAAS annotation

Expressioni

Gene expression databases

BgeeiENSRNOG00000016325.

Interactioni

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 90GlaInterPro annotationAdd BLAST47
Domaini108 – 187KringleInterPro annotationAdd BLAST80
Domaini214 – 292KringleInterPro annotationAdd BLAST79
Domaini360 – 614Peptidase S1InterPro annotationAdd BLAST255

Sequence similaritiesi

Belongs to the peptidase S1 family.UniRule annotationSAAS annotation

Keywords - Domaini

KringlePROSITE-ProRule annotationSAAS annotation, SignalSequence analysis

Phylogenomic databases

eggNOGiENOG410IKPN. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119133.
KOiK01313.
OMAiGIECQLW.
OrthoDBiEOG091G0AH5.
TreeFamiTF327329.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di4.10.140.10. 1 hit.
4.10.740.10. 1 hit.
InterProiView protein in InterPro
IPR017857. Coagulation_fac_subgr_Gla_dom.
IPR035972. GLA-like_dom_SF.
IPR000294. GLA_domain.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR003966. Prothrombin/thrombin.
IPR018992. Thrombin_light_chain.
IPR037111. Thrombin_light_chain_sf.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
PANTHERiPTHR24254:SF10. PTHR24254:SF10. 1 hit.
PfamiView protein in Pfam
PF00594. Gla. 1 hit.
PF00051. Kringle. 2 hits.
PF09396. Thrombin_light. 1 hit.
PF00089. Trypsin. 1 hit.
PIRSFiPIRSF001149. Thrombin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
PR00001. GLABLOOD.
PR01505. PROTHROMBIN.
SMARTiView protein in SMART
SM00069. GLA. 1 hit.
SM00130. KR. 2 hits.
SM00020. Tryp_SPc. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 2 hits.
SSF57630. SSF57630. 1 hit.
PROSITEiView protein in PROSITE
PS00011. GLA_1. 1 hit.
PS50998. GLA_2. 1 hit.
PS00021. KRINGLE_1. 2 hits.
PS50070. KRINGLE_2. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

G3V843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHVRGLGLP GCLALAALAS LVHSQHVFLA PQQALSLLQR VRRANSGFLE
60 70 80 90 100
ELRKGNLERE CVEEQCSYEE AFEALESPQD TDVFWAKYTV CDSVRKPRET
110 120 130 140 150
FMDCLEGRCA MDLGLNYHGN VSVTHTGIEC QLWRSRYPHR PDINSTTHPG
160 170 180 190 200
ADLKENFCRN PDSSTSGPWC YTTDPTVRRE ECSIPVCGQE GRTTVKMTPR
210 220 230 240 250
SRGSKENLSP PLGECLLERG RLYQGNLAVT TLGSPCLAWD SLPTKTLSKY
260 270 280 290 300
QNFDPEVKLV QNFCRNPDRD EEGAWCFVAQ QPGFEYCSLN YCDEAVGEEN
310 320 330 340 350
HDGDESIAGR TTDAEFHTFF DERTFGLGEA DCGLRPLFEK KSLTDKTEKE
360 370 380 390 400
LLDSYIDGRI VEGWDAEKGI APWQVMLFRK SPQELLCGAS LISDRWVLTA
410 420 430 440 450
AHCILYPPWD KNFTENDLLV RIGKHSRTRY ERNVEKISML EKIYIHPRYN
460 470 480 490 500
WRENLDRDIA LLKLKKPVPF SDYIHPVCLP DKQTVTSLLQ AGYKGRVTGW
510 520 530 540 550
GNLRETWTTN INEIQPSVLQ VVNLPIVERP VCKASTRIRI TDNMFCAGFK
560 570 580 590 600
VNDTKRGDAC EGDSGGPFVM KSPYNHRWYQ MGIVSWGEGC DRNGKYGFYT
610
HVFRLKRWMQ KVIDQHR
Length:617
Mass (Da):70,386
Last modified:November 16, 2011 - v1
Checksum:i8C11117828B20AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR07052881 Genomic DNA. No translation available.
CH473949 Genomic DNA. Translation: EDL79531.1.
RefSeqiNP_075213.2. NM_022924.2.
UniGeneiRn.54498.

Genome annotation databases

EnsembliENSRNOT00000022233; ENSRNOP00000022233; ENSRNOG00000016325.
GeneIDi29251.
KEGGirno:29251.

Similar proteinsi

Entry informationi

Entry nameiG3V843_RAT
AccessioniPrimary (citable) accession number: G3V843
Entry historyiIntegrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: November 22, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.PROSITE-ProRule annotation

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported