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Protein

Neurotrypsin

Gene

Prss12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei516 – 517Reactive bond homologSequence analysis2
Active sitei562Charge relay systemBy similarity1
Active sitei612Charge relay systemBy similarity1
Active sitei711Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.237.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurotrypsin (EC:3.4.21.-)
Alternative name(s):
Serine protease 12
Gene namesi
Name:Prss12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi69238. Prss12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000041684322 – 761NeurotrypsinAdd BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi85 ↔ 1571 Publication
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi101 ↔ 1411 Publication
Disulfide bondi130 ↔ 1551 Publication
Disulfide bondi191 ↔ 255By similarity
Disulfide bondi204 ↔ 265By similarity
Disulfide bondi235 ↔ 245By similarity
Disulfide bondi298 ↔ 361By similarity
Disulfide bondi311 ↔ 371By similarity
Disulfide bondi341 ↔ 351By similarity
Disulfide bondi411 ↔ 475By similarity
Disulfide bondi424 ↔ 485By similarity
Disulfide bondi455 ↔ 465By similarity
Disulfide bondi505 ↔ 636Sequence analysis
Glycosylationi521N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi547 ↔ 563By similarity
Glycosylationi569N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi651 ↔ 717By similarity
Disulfide bondi680 ↔ 694By similarity
Disulfide bondi707 ↔ 736By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiG3V801.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015353.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021116.

Structurei

Secondary structure

1761
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi92 – 95Combined sources4
Beta strandi98 – 101Combined sources4
Helixi104 – 106Combined sources3
Helixi119 – 121Combined sources3
Beta strandi131 – 134Combined sources4
Turni135 – 137Combined sources3
Beta strandi138 – 144Combined sources7
Beta strandi150 – 154Combined sources5
Beta strandi156 – 158Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K4RNMR-A84-160[»]
2K51NMR-A84-160[»]
ProteinModelPortaliG3V801.
SMRiG3V801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 157KringlePROSITE-ProRule annotationAdd BLAST73
Domaini166 – 267SRCR 1PROSITE-ProRule annotationAdd BLAST102
Domaini273 – 373SRCR 2PROSITE-ProRule annotationAdd BLAST101
Domaini386 – 487SRCR 3PROSITE-ProRule annotationAdd BLAST102
Domaini517 – 760Peptidase S1PROSITE-ProRule annotationAdd BLAST244

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni505 – 516Zymogen activation regionBy similarityAdd BLAST12

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 3 SRCR domains.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00840000129699.
HOGENOMiHOG000113767.
InParanoidiG3V801.
KOiK09624.
OMAiRYGNSTR.
OrthoDBiEOG091G0DF7.
PhylomeDBiG3V801.
TreeFamiTF329295.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 3 hits.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00530. SRCR. 3 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
PR00258. SPERACTRCPTR.
SMARTiSM00130. KR. 1 hit.
SM00202. SR. 3 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 3 hits.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 3 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G3V801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALARCVLAV ILGVLSEVAR ADPVLHSPLH RPHPSPPRSQ HAHYLPSSRR
60 70 80 90 100
PPRTPRFPLP PRAPAAQRPQ LLSTRHTPPT ISRRCGAGEP WGNATNLGVP
110 120 130 140 150
CLHWDEVPPF LERSPPASWA ELRGQPHNFC RSPGGAGRPW CFYRNAQGKV
160 170 180 190 200
DWGYCDCGQG PALPVIRLVG GKSGHEGRVE LYHAGQWGTI CDDQWDDADA
210 220 230 240 250
EVICRQLGLS GIAKAWHQAH FGEGSGPILL DEVRCTGNEL SIEQCPKSSW
260 270 280 290 300
GEHNCGHKED AGVSCAPLTD GVIRLSGGKS VHEGRLEVYY RGQWGTVCDD
310 320 330 340 350
GWTEMNTYVA CRLLGFKYGK QSSVNHFEGS SRPIWLDDVS CSGKEASFIQ
360 370 380 390 400
CSRRQWGRHD CSHREDVGLT CYPDSDGHRL SPGFPIRLMD GENKREGRVE
410 420 430 440 450
VFVSGQWGTI CDDGWTDKHA AVICRQLGYK GPARARTMAY FGEGKGPIHM
460 470 480 490 500
DNVKCTGNEK ALADCVKQDI GRHNCRHSED AGVICDYYEK KTSGHGNKET
510 520 530 540 550
LSSGCGLRLL HRRQKRIIGG NNSLRGAWPW QASLRLKSTH GDGRLLCGAT
560 570 580 590 600
LLSSCWVLTA AHCFKRYGNN SRSYAVRVGD YHTLVPEEFE QEIGVQQIVI
610 620 630 640 650
HRNYRPDSSD YDIALVRLQG SGEQCARLST HVLPACLPLW RERPQKTASN
660 670 680 690 700
CHITGWGDTG RAYSRTLQQA AVPLLPKRFC KERYKGLFTG RMLCAGNLQE
710 720 730 740 750
DNRVDSCQGD SGGPLMCEKP DETWVVYGVT SWGYGCGIKD TPGVYTRVPA
760
FVPWIKSVTS L
Length:761
Mass (Da):84,220
Last modified:November 16, 2011 - v1
Checksum:i215EE73A3CE468EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134G → D in CAC35028 (Ref. 1) Curated1
Sequence conflicti404S → N in CAC35028 (Ref. 1) Curated1
Sequence conflicti515K → T in CAC35028 (Ref. 1) Curated1
Sequence conflicti565K → T in CAC35028 (Ref. 1) Curated1
Sequence conflicti588E → G in CAC35028 (Ref. 1) Curated1
Sequence conflicti592E → D in CAC35028 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311671 mRNA. Translation: CAC35028.2.
CH473952 Genomic DNA. Translation: EDL82129.1.
RefSeqiNP_445956.1. NM_053504.1.
UniGeneiRn.86653.

Genome annotation databases

EnsembliENSRNOT00000021116; ENSRNOP00000021116; ENSRNOG00000015353.
GeneIDi85266.
KEGGirno:85266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311671 mRNA. Translation: CAC35028.2.
CH473952 Genomic DNA. Translation: EDL82129.1.
RefSeqiNP_445956.1. NM_053504.1.
UniGeneiRn.86653.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K4RNMR-A84-160[»]
2K51NMR-A84-160[»]
ProteinModelPortaliG3V801.
SMRiG3V801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021116.

Protein family/group databases

MEROPSiS01.237.

Proteomic databases

PaxDbiG3V801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021116; ENSRNOP00000021116; ENSRNOG00000015353.
GeneIDi85266.
KEGGirno:85266.

Organism-specific databases

CTDi8492.
RGDi69238. Prss12.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00840000129699.
HOGENOMiHOG000113767.
InParanoidiG3V801.
KOiK09624.
OMAiRYGNSTR.
OrthoDBiEOG091G0DF7.
PhylomeDBiG3V801.
TreeFamiTF329295.

Miscellaneous databases

PROiG3V801.

Gene expression databases

BgeeiENSRNOG00000015353.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 3 hits.
InterProiIPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00530. SRCR. 3 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
PR00258. SPERACTRCPTR.
SMARTiSM00130. KR. 1 hit.
SM00202. SR. 3 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 3 hits.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS00420. SRCR_1. 3 hits.
PS50287. SRCR_2. 3 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNETR_RAT
AccessioniPrimary (citable) accession number: G3V801
Secondary accession number(s): Q99JC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 16, 2011
Last modified: November 2, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.