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Protein

Syntaxin-12

Gene

Stx12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE that acts to regulate protein transport between endosomes and the trans-Golgi network (By similarity). The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-12
Alternative name(s):
Syntaxin-13
Gene namesi
Name:Stx12
Synonyms:Stx13
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620977. Stx12.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 250CytoplasmicSequence analysisAdd BLAST249
Transmembranei251 – 271Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini272 – 274VesicularSequence analysis3

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004154992 – 274Syntaxin-12Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei139PhosphoserineBy similarity1
Modified residuei142PhosphoserineCombined sources1
Modified residuei218PhosphoserineBy similarity1
Modified residuei225PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiG3V7P1.
PRIDEiG3V7P1.

PTM databases

iPTMnetiG3V7P1.
PhosphoSitePlusiG3V7P1.
SwissPalmiG3V7P1.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in brain.2 Publications

Gene expression databases

BgeeiENSRNOG00000011804.
ExpressionAtlasiG3V7P1. baseline and differential.
GenevisibleiG3V7P1. RN.

Interactioni

Subunit structurei

Associates with the BLOC-1 complex. Interacts with BLOC1S6 (By similarity). Interacts with NAPA and SNAP23. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Vamp1Q636663EBI-915654,EBI-2029956

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249226. 1 interactor.
IntActiG3V7P1. 5 interactors.
MINTiMINT-5023130.
STRINGi10116.ENSRNOP00000017227.

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi184 – 245Combined sources62

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50B184-251[»]
SMRiG3V7P1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 240t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili34 – 80Sequence analysisAdd BLAST47

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
GeneTreeiENSGT00450000040239.
HOGENOMiHOG000188453.
HOVERGENiHBG053083.
InParanoidiG3V7P1.
KOiK13813.
PhylomeDBiG3V7P1.
TreeFamiTF315607.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G3V7P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYGPLDMYR NPGPSGPQPR DFNSIIQTCS GNIQRISQAT AQIKNLMSQL
60 70 80 90 100
GTKQDSSKLQ ENLQQFQHST NQLAKETNEL LKELGSLPLP LSASEQRQQK
110 120 130 140 150
LQKERLMNDF SSALNNFQVV QRKVSEKEKE SIARARAGSR LSAEDRQREE
160 170 180 190 200
QLVSFDSHEE WNQMQSQEEE AAITEQDLEL IKERETAIQQ LEADILDVNQ
210 220 230 240 250
IFKDLAMMIH DQGDLIDSIE ANVESSEVHV ERASDQLQRA AYYQKKSRKK
260 270
MCILVLVLSV IVTVLVVVIW VASK
Length:274
Mass (Da):31,187
Last modified:November 16, 2011 - v1
Checksum:iD5D551B57776E195
GO

Sequence cautioni

The sequence AAC18967 differs from that shown. Reason: Frameshift at position 2.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14 – 19PSGPQP → RRSL in AAC23484 (PubMed:9507000).Curated6
Sequence conflicti17 – 18PQ → LR in AAC18967 (PubMed:9553086).Curated2
Sequence conflicti23N → S in AAC23484 (PubMed:9507000).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035632 mRNA. Translation: AAC23484.1.
AF044581 mRNA. Translation: AAC18967.1. Frameshift.
CH473968 Genomic DNA. Translation: EDL80653.1.
RefSeqiNP_075228.2. NM_022939.2.
UniGeneiRn.54570.

Genome annotation databases

EnsembliENSRNOT00000017227; ENSRNOP00000017227; ENSRNOG00000011804.
GeneIDi65033.
KEGGirno:65033.
UCSCiRGD:620977. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035632 mRNA. Translation: AAC23484.1.
AF044581 mRNA. Translation: AAC18967.1. Frameshift.
CH473968 Genomic DNA. Translation: EDL80653.1.
RefSeqiNP_075228.2. NM_022939.2.
UniGeneiRn.54570.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50B184-251[»]
SMRiG3V7P1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249226. 1 interactor.
IntActiG3V7P1. 5 interactors.
MINTiMINT-5023130.
STRINGi10116.ENSRNOP00000017227.

PTM databases

iPTMnetiG3V7P1.
PhosphoSitePlusiG3V7P1.
SwissPalmiG3V7P1.

Proteomic databases

PaxDbiG3V7P1.
PRIDEiG3V7P1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017227; ENSRNOP00000017227; ENSRNOG00000011804.
GeneIDi65033.
KEGGirno:65033.
UCSCiRGD:620977. rat.

Organism-specific databases

CTDi23673.
RGDi620977. Stx12.

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
GeneTreeiENSGT00450000040239.
HOGENOMiHOG000188453.
HOVERGENiHBG053083.
InParanoidiG3V7P1.
KOiK13813.
PhylomeDBiG3V7P1.
TreeFamiTF315607.

Miscellaneous databases

PROiG3V7P1.

Gene expression databases

BgeeiENSRNOG00000011804.
ExpressionAtlasiG3V7P1. baseline and differential.
GenevisibleiG3V7P1. RN.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX12_RAT
AccessioniPrimary (citable) accession number: G3V7P1
Secondary accession number(s): O70319, O88385
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: November 16, 2011
Last modified: November 2, 2016
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.