G3V7P1 (STX12_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-12

Alternative name(s):
Syntaxin-13

Gene names

Name:	Stx12
Synonyms:	Stx13

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE that acts to regulate protein transport between endosomes and the trans-Golgi network By similarity. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro). Ref.5
Subunit structure	Associates with the BLOC-1 complex. Interacts with BLOC1S6 By similarity. Interacts with NAPA and SNAP23. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A. Ref.1 Ref.5
Subcellular location	Endosome membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Endomembrane system; Single-pass type IV membrane protein; Cytoplasmic side Potential Ref.1 Ref.2.
Tissue specificity	Ubiquitous. Highly expressed in brain. Ref.1 Ref.2
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.
Sequence caution	The sequence AAC18967.1 differs from that shown. Reason: Frameshift at position 2.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol efflux Inferred from electronic annotation. Source: Compara intracellular protein transport Inferred from Biological aspect of Ancestor. Source: RefGenome protein stabilization Inferred from electronic annotation. Source: Compara synaptic vesicle exocytosis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	BLOC-1 complex Inferred from electronic annotation. Source: Compara Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell SNARE complex Inferred from sequence or structural similarity. Source: UniProtKB endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from electronic annotation. Source: Compara phagocytic vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	SNAP receptor activity Inferred from Biological aspect of Ancestor. Source: RefGenome SNARE binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 274

274

Syntaxin-12

PRO_0000415499

Regions

Topological domain

1 – 250

250

Cytoplasmic Potential

Transmembrane

251 – 271

21

Helical; Anchor for type IV membrane protein; Potential

Topological domain

272 – 274

3

Vesicular Potential

Domain

178 – 240

63

t-SNARE coiled-coil homology

Coiled coil

34 – 80

47

Potential

Amino acid modifications

Modified residue

139

1

Phosphoserine By similarity

Modified residue

142

1

Phosphoserine By similarity

Modified residue

218

1

Phosphoserine By similarity

Experimental info

Sequence conflict

14 – 19

6

PSGPQP → RRSL in AAC23484. Ref.1

Sequence conflict

17 – 18

2

PQ → LR in AAC18967. Ref.2

Sequence conflict

23

1

N → S in AAC23484. Ref.1

Secondary structure

1

.

.

.

274

Helix Strand Turn

Details...

Helix

184 – 245

62

Sequences

	Sequence		Length	Mass (Da)
	G3V7P1 [UniParc]. Last modified November 16, 2011. Version 1. Checksum: D5D551B57776E195	FASTA	274	31,187
	10 20 30 40 50 60 MSYGPLDMYR NPGPSGPQPR DFNSIIQTCS GNIQRISQAT AQIKNLMSQL GTKQDSSKLQ 70 80 90 100 110 120 ENLQQFQHST NQLAKETNEL LKELGSLPLP LSASEQRQQK LQKERLMNDF SSALNNFQVV 130 140 150 160 170 180 QRKVSEKEKE SIARARAGSR LSAEDRQREE QLVSFDSHEE WNQMQSQEEE AAITEQDLEL 190 200 210 220 230 240 IKERETAIQQ LEADILDVNQ IFKDLAMMIH DQGDLIDSIE ANVESSEVHV ERASDQLQRA 250 260 270 AYYQKKSRKK MCILVLVLSV IVTVLVVVIW VASK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Syntaxin 12, a member of the syntaxin family localized to the endosome." Tang B.L., Tan A.E., Lim L.K., Lee S.S., Low D.Y., Hong W. J. Biol. Chem. 273:6944-6950(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH WITH NAPA AND SNAP23, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Seven novel mammalian SNARE proteins localize to distinct membrane compartments." Advani R.J., Bae H.-R., Bock J.B., Chao D.S., Doung Y.-C., Prekeris R., Yoo J.-S., Scheller R.H. J. Biol. Chem. 273:10317-10324(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[5]	"Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies." Zwilling D., Cypionka A., Pohl W.H., Fasshauer D., Walla P.J., Wahl M.C., Jahn R. EMBO J. 26:9-18(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 184-251 N COMPLEX WITH STX6; VTI1A AND VAMP4, FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF035632 mRNA. Translation: AAC23484.1.
AF044581 mRNA. Translation: AAC18967.1. Frameshift.
CH473968 Genomic DNA. Translation: EDL80653.1.

IPI

IPI00208759.
IPI00947964.

RefSeq

NP_075228.2. NM_022939.2.

UniGene

Rn.54570.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NPS	X-ray	2.50	B	184-251	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

G3V7P1. 1 interaction.

STRING

10116.ENSRNOP00000017227.

PTM databases

PhosphoSite

G3V7P1.

Proteomic databases

PRIDE

G3V7P1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000017227; ENSRNOP00000017227; ENSRNOG00000011804.

GeneID

65033.

KEGG

rno:65033.

Organism-specific databases

CTD

23673.

RGD

620977. Stx12.

Phylogenomic databases

eggNOG

COG5325.

GeneTree

ENSGT00450000040239.

HOGENOM

HOG000188453.

HOVERGEN

HBG053083.

KO

K13813.

OMA

CHEEWNQ.

Family and domain databases

InterPro

IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]

Pfam

PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]

SMART

SM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O70319.

NextBio

613828.

Entry information

Entry name

STX12_RAT

Accession

Primary (citable) accession number: G3V7P1
Secondary accession number(s): O70319, O88385

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	November 16, 2011
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program