ID   G3UWE4_MOUSE            Unreviewed;       502 AA.
AC   G3UWE4;
DT   16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT   16-NOV-2011, sequence version 1.
DT   27-MAR-2024, entry version 78.
DE   RecName: Full=steroid 11beta-monooxygenase {ECO:0000256|ARBA:ARBA00012767};
DE            EC=1.14.15.4 {ECO:0000256|ARBA:ARBA00012767};
GN   Name=Cyp11b2 {ECO:0000313|Ensembl:ENSMUSP00000131503.2,
GN   ECO:0000313|MGI:MGI:88584};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000131503.2, ECO:0000313|Proteomes:UP000000589};
RN   [1] {ECO:0000313|Ensembl:ENSMUSP00000131503.2, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000131503.2,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2] {ECO:0000313|Ensembl:ENSMUSP00000131503.2}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000131503.2};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=21-hydroxyprogesterone + 2 H(+) + O2 + 2 reduced [adrenodoxin]
CC         = 18-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin];
CC         Xref=Rhea:RHEA:76151, Rhea:RHEA-COMP:9998, Rhea:RHEA-COMP:9999,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16973, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC         ChEBI:CHEBI:195166; Evidence={ECO:0000256|ARBA:ARBA00035593};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:76152;
CC         Evidence={ECO:0000256|ARBA:ARBA00035593};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=21-hydroxyprogesterone + 2 H(+) + O2 + 2 reduced [adrenodoxin]
CC         = corticosterone + H2O + 2 oxidized [adrenodoxin];
CC         Xref=Rhea:RHEA:46104, Rhea:RHEA-COMP:9998, Rhea:RHEA-COMP:9999,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16827, ChEBI:CHEBI:16973, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738; Evidence={ECO:0000256|ARBA:ARBA00035574};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46105;
CC         Evidence={ECO:0000256|ARBA:ARBA00035574};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a steroid + 2 H(+) + O2 + 2 reduced [adrenodoxin] = an 11beta-
CC         hydroxysteroid + H2O + 2 oxidized [adrenodoxin];
CC         Xref=Rhea:RHEA:15629, Rhea:RHEA-COMP:9998, Rhea:RHEA-COMP:9999,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:35341,
CC         ChEBI:CHEBI:35346; EC=1.14.15.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00035575};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15630;
CC         Evidence={ECO:0000256|ARBA:ARBA00035575};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000256|ARBA:ARBA00001971,
CC         ECO:0000256|PIRSR:PIRSR602399-1};
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC       {ECO:0000256|ARBA:ARBA00010617, ECO:0000256|RuleBase:RU000461}.
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DR   RefSeq; NP_034121.3; NM_009991.3.
DR   AlphaFoldDB; G3UWE4; -.
DR   SMR; G3UWE4; -.
DR   MaxQB; G3UWE4; -.
DR   ProteomicsDB; 339292; -.
DR   DNASU; 13072; -.
DR   Ensembl; ENSMUST00000167634.2; ENSMUSP00000131503.2; ENSMUSG00000022589.9.
DR   GeneID; 13072; -.
DR   KEGG; mmu:13072; -.
DR   UCSC; uc007wgf.2; mouse.
DR   AGR; MGI:88584; -.
DR   CTD; 1585; -.
DR   MGI; MGI:88584; Cyp11b2.
DR   VEuPathDB; HostDB:ENSMUSG00000022589; -.
DR   GeneTree; ENSGT00940000161506; -.
DR   HOGENOM; CLU_001570_28_4_1; -.
DR   OMA; RNHKCGV; -.
DR   OrthoDB; 2658719at2759; -.
DR   PhylomeDB; G3UWE4; -.
DR   TreeFam; TF105094; -.
DR   BioGRID-ORCS; 13072; 9 hits in 79 CRISPR screens.
DR   Proteomes; UP000000589; Chromosome 15.
DR   Bgee; ENSMUSG00000022589; Expressed in adrenal gland and 20 other cell types or tissues.
DR   ExpressionAtlas; G3UWE4; baseline and differential.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004507; F:steroid 11-beta-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002399; Cyt_P450_mitochondrial.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   PANTHER; PTHR24279; -; 1.
DR   PANTHER; PTHR24279:SF1; CYTOCHROME P450 11B2, MITOCHONDRIAL; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00408; MITP450.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; Cytochrome P450; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Heme {ECO:0000256|ARBA:ARBA00022617, ECO:0000256|PIRSR:PIRSR602399-1};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR602399-1};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR602399-1};
KW   Monooxygenase {ECO:0000256|ARBA:ARBA00023033,
KW   ECO:0000256|RuleBase:RU000461};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|RuleBase:RU000461};
KW   Proteomics identification {ECO:0007829|MaxQB:G3UWE4,
KW   ECO:0007829|ProteomicsDB:G3UWE4};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Steroidogenesis {ECO:0000256|ARBA:ARBA00023250};
KW   Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT   BINDING         383
FT                   /ligand="21-hydroxyprogesterone"
FT                   /ligand_id="ChEBI:CHEBI:16973"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR602399-1"
FT   BINDING         449
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR602399-1"
SQ   SEQUENCE   502 AA;  57575 MW;  BCAFFF3EE471ED37 CRC64;
     MAMALRVTAD VWLARPWQCL HRTRALGTTA TLAPKTLQPF EAIPQYSRNK WLKMIQILRE
     QGQENLHLEM HQVFRELGPI FRHSVGKTQI VSVMLPEDAE KLHQVESMLP RRMHLEPWVA
     HRELRGLRRG VFLLNGPEWR LNRLRLNRNV LSPKAVQKFV PMVDMVARDF LETLKEKVLQ
     NARGSLTMDV QQSLFNYTIE ASNFALFGER LGLLGHDLSP GSLKFIHALH SMFKSTSQLL
     FLPKSLTRWT STRVWKEHFD AWDVISEYAN RCIWKVHQEL RLGSSQTYSG IVAELISQGS
     LPLDAIKANS MELTAGSVDT TAIPLVMTLF ELARNPDVQK ALRQESLAAE ASIAANPQKA
     MSDLPLLRAA LKETLRLYPV GGFLERILSS DLVLQNYHVP AGTLVLLYLY SMGRNPAVFP
     RPERYMPQRW LERKRSFQHL AFGFGVRQCL GRRLAEVEMM LLLHHILKTF QVETLRQEDV
     QMAYRFVLMP SSSPVLTFRP VS
//