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Protein

Cis-abienol synthase, chloroplastic

Gene

ABS

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of cis-abienol, a labdane diterpene that can be used as synthesis precursor of ambergris substitution fragance products.1 Publication

Miscellaneous

The expression of both CPS2 and ABS is necessary and sufficient to catalyze the biosynthesis of cis-abienol from geranylgeranyl diphosphate in a heterologous system.1 Publication

Catalytic activityi

(13E)-8-alpha-hydroxylabd-13-en-15-yl diphosphate = cis-abienol + diphosphate.1 Publication

Cofactori

Mg2+Curated

Pathwayi: terpenoid biosynthesis

This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi539Magnesium 1By similarity1
Metal bindingi539Magnesium 2By similarity1
Metal bindingi543Magnesium 1By similarity1
Metal bindingi543Magnesium 2By similarity1
Metal bindingi684Magnesium 3By similarity1
Metal bindingi692Magnesium 3By similarity1

GO - Molecular functioni

  • carbon-oxygen lyase activity, acting on phosphates Source: UniProtKB
  • magnesium ion binding Source: InterPro
  • terpene synthase activity Source: UniProtKB

GO - Biological processi

  • cis-abienol biosynthetic process Source: UniProtKB
  • copal-8-ol diphosphate(3-) catabolic process Source: UniProtKB

Keywordsi

Molecular functionLyase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00213.

Names & Taxonomyi

Protein namesi
Recommended name:
Cis-abienol synthase, chloroplastic (EC:4.2.3.140)
Short name:
NtABS
Gene namesi
Name:ABS
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37ChloroplastSequence analysisAdd BLAST37
ChainiPRO_500079311738 – 792Cis-abienol synthase, chloroplasticAdd BLAST755

Expressioni

Tissue specificityi

Expressed specifically in trichomes.1 Publication

Structurei

3D structure databases

ProteinModelPortaliG3CCC1.
SMRiG3CCC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi539 – 543DDXXD motif5

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK04121.
K20517.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.130. 1 hit.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR036965. Terpene_synth_N_sf.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
PfamiView protein in Pfam
PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G3CCC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLGLRSKII PLPDHKLGNI KLGSVTNAIC HRPCRVRCSH STASSMEEAK
60 70 80 90 100
ERIRETFGKI ELSPSSYDTA WVAMVPSRYS MNQPCFPQCL DWILENQRED
110 120 130 140 150
GSWGLNPSHP LLVKDSLSST LASLLALRKW RIGDNQVQRG LGFIETHGWA
160 170 180 190 200
VDNKDQISPL GFEIIFPCMI NYAEKLNLDL PLDPNLVNMM LCERELTIER
210 220 230 240 250
ALKNEFEGNM ANVEYFAEGL GELCHWKEMM LRQRHNGSLF DSPATTAAAL
260 270 280 290 300
IYHQYDEKCF GYLNSILKLH DNWVPTICPT KIHSNLFLVD ALQNLGVDRY
310 320 330 340 350
FKTEVKRVLD EIYRLWLEKN EEIFSDVAHC AMAFRLLRMN NYEVSSEELE
360 370 380 390 400
GFVDQEHFFT TSSGKLMNHV AILELHRASQ VAIHERKDHI LDKISTWTRN
410 420 430 440 450
FMEQKLLDKH IPDRSKKEME FAMRKFYGTF DRVETRRYIE SYKMDSFKIL
460 470 480 490 500
KAAYRSSGIN NIDLLKFSEH DFNLCQTRHK EELQQMKRWF TDCKLEQVGL
510 520 530 540 550
SQQYLYTSYF IIAAILFEPE YADARLAYAK YAIIITAVDD FFDCFICKEE
560 570 580 590 600
LQNIIELVER WEGYSTVGFR SERVRIFFLA LYKMVEEIAA KAETKQGRCV
610 620 630 640 650
KDHLINLWID MLKCMLVELD LWKIKSTTPS IEEYLSVACV TIGVPCFVLT
660 670 680 690 700
SLYLLGPKLS KDVIESSEVS ALCNCTAAVA RLINDIHSYK REQAESSTNM
710 720 730 740 750
VSILITQSQG TISEEEAIRQ IKEMMESKRR ELLGMVLQNK ESQLPQVCKD
760 770 780 790
LFWTTINAAY SIHTHGDGYR FPEEFKNHIN DVIYKPLNQY SP
Length:792
Mass (Da):91,772
Last modified:November 16, 2011 - v1
Checksum:iDC0034030033395C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE588140 mRNA. Translation: CCD33019.1.
KF000066 mRNA. Translation: AGN95433.1.
RefSeqiNP_001312911.1. NM_001325982.1.
UniGeneiNta.4112.

Genome annotation databases

GeneIDi107816701.
KEGGiag:CCD33019.
nta:107816701.

Similar proteinsi

Entry informationi

Entry nameiCABS_TOBAC
AccessioniPrimary (citable) accession number: G3CCC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: November 16, 2011
Last modified: October 25, 2017
This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families