G2TJ47 (G2TJ47_BACCO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420
EC=6.3.5.3 HAMAP-Rule MF_00420

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II HAMAP-Rule MF_00420

Gene names

Name:	purL HAMAP-Rule MF_00420
ORF Names:	Bcoa_1613 EMBL AEP00810.1

Organism

Bacillus coagulans 36D1 EMBL AEP00810.1

Taxonomic identifier

345219 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus coagulans

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity. HAMAP-Rule MF_00420
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00420.
Sequence similarities	Belongs to the FGAMS family. HAMAP-Rule MF_00420

Ontologies

Keywords
Biological process	Purine biosynthesis HAMAP-Rule MF_00420
Cellular component	Cytoplasm HAMAP-Rule MF_00420
Ligand	ATP-binding HAMAP-Rule MF_00420 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00420
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	111 – 122	12	ATP By similarity HAMAP-Rule MF_00420

Sequences

	Sequence		Length	Mass (Da)
	G2TJ47 [UniParc]. Last modified November 16, 2011. Version 1. Checksum: 77AA7B49342597E2	FASTA	749	81,069
	10 20 30 40 50 60 MPLLLEPGPA EIKSQKIYRE IGLTDEEFAR VEAILGRTPN YTETGLFAVM WSEHCSYKNS 70 80 90 100 110 120 KPVLKKFPVK GERVLQGPGE GAGIIDIGDG LAVVFKIESH NHPSAIEPYQ GAATGVGGII 130 140 150 160 170 180 RDVFSMGARP VALLNSLRFG ELDSPRVKHL FKEVVAGIAG YGNCIGIPTV GGEVAFDPCY 190 200 210 220 230 240 DGNPLVNAMC VGLIEHKDIQ KGQARGIGNT VMYVGAKTGR DGIHGATFAS GQLSEDSDEK 250 260 270 280 290 300 RPAVQVGDPF MEKLLLEACL ELVRHDALVG IQDMGAAGLI SSSSEMASKA GVGLVMNLDR 310 320 330 340 350 360 IPQRETGMTP YEMMLSESQE RMLIVVKKGR EPEIIRLFQK YGLDAVAIGT VTDDKRLKLY 370 380 390 400 410 420 HHGELVADVP ADALAEDAPV YHKPSKEPAY FAAYQVMKPQ IPAVENLQET FFQLLKQPTI 430 440 450 460 470 480 ASKEWVYEQY DYQVRTNTVV MPGSDAAVIR IRGTRKALAM TTDCNARYVY LDPETGGKIA 490 500 510 520 530 540 VAEAARNIVC SGAQPLGVTD CLNFGAPEKP EIFWQLEKAA DGMSEACRAL ETPVIGGNVS 550 560 570 580 590 600 LYNETLQKTG NGEVSVQAVY PTPIVGMVGL LEDITHATTQ TFKQAGDLIY LIGEAKPEFG 610 620 630 640 650 660 GSELQKLTDG QISGKPPALD LETEARREHQ LLAAIQSGLV QSAHDVSEGG FAPALAECLF 670 680 690 700 710 720 GTENLGAHVV LKGNPVTALF SETQSRFIVS VKKEHQQVFQ NLVNEAVWIG HVTDDKQMTI 730 740 KGENGETWIL ADTAECETAW KGALRCLLK

References

[1]

"Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1." Rhee M.S., Moritz B.E., Xie G., Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Chertkov O., Brettin T., Han C., Detter C., Pitluck S., Richardson P. , Land M.L., Patel M., Ou M., Harbrucker R., Ingram L.O., Shanmugam K.T. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 36D1 EMBL AEP00810.1.

[2]

Shanmugan K.T. Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 36D1 EMBL AEP00810.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003056 Genomic DNA. Translation: AEP00810.1.
RefSeq	YP_004859590.1. NC_016023.1.
3D structure databases
ProteinModelPortal	G2TJ47.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEP00810; AEP00810; Bcoa_1613.
GeneID	11171899.
KEGG	bag:Bcoa_1613.
Phylogenomic databases
KO	K01952.
Enzyme and pathway databases
BioCyc	BCOA345219:GH5A-1591-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G2TJ47_BACCO

Accession

Primary (citable) accession number: G2TJ47

Entry history

Integrated into UniProtKB/TrEMBL:	November 16, 2011
Last sequence update:	November 16, 2011
Last modified:	April 3, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)