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G2THX3 (G2THX3_BACCO) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:Bcoa_1485 EMBL AEP00691.1
OrganismBacillus coagulans 36D1 [Complete proteome] EMBL AEP00691.1
Taxonomic identifier345219 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 339206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding167 – 22256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2931Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G2THX3 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: EA90AE643122BBA5

FASTA35539,463
        10         20         30         40         50         60 
MKKKICLLYG GKSAEHEVSL QTAKAVIQAL DHEKYDIYPI FITTEGDWIK GPLLTGPVET 

        70         80         90        100        110        120 
VKQLEFRDGK KMAPNALSTT VSGEKGGYDV IFPLLHGPNG EDGTVQGMLE LLNLPYVGNG 

       130        140        150        160        170        180 
VLASSAGMDK VIMKNLFAQA GLDQVKYVSF IRSEWENALE TCFQQVEEKT GYPCFVKPAN 

       190        200        210        220        230        240 
LGSSVGISKC KNRTELEAAF KEAFQYDRKI IVEEGVTARE IELGVIGNDD PQCSVAGEIV 

       250        260        270        280        290        300 
AKKDFYDYKA KYEDGETALI IPADIPEKVY KKCTEAAITA FKALDCSGLV RADFFVTEDE 

       310        320        330        340        350 
RVIINEINTM PGFTPFSMFP LLWKEAGLDY PQLIEKLIEL ALERHAEKQR IKYTL 

« Hide

References

[1]US DOE Joint Genome Institute (JGI-PGF)
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Chertkov O., Brettin T., Han C., Detter C., Pitluck S., Shanmugam K.T.
Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 36D1 EMBL AEP00691.1.
[2]"Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1."
Rhee M.S., Moritz B.E., Xie G., Glavina Del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Chertkov O., Brettin T., Han C., Detter C., Pitluck S., Land M.L., Patel M., Ou M., Harbrucker R., Ingram L.O., Shanmugam K.T.
Stand. Genomic Sci. 5:331-340(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 36D1 EMBL AEP00691.1.
[3]Shanmugan K.T.
Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 36D1 EMBL AEP00691.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003056 Genomic DNA. Translation: AEP00691.1.
RefSeqYP_004859471.1. NC_016023.1.

3D structure databases

ProteinModelPortalG2THX3.
SMRG2THX3. Positions 2-355.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEP00691; AEP00691; Bcoa_1485.
GeneID11171778.
KEGGbag:Bcoa_1485.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycBCOA345219:GH5A-1470-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2THX3_BACCO
AccessionPrimary (citable) accession number: G2THX3
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: February 19, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)