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G2T1L3 (G2T1L3_ROSHA) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108
Gene names
Ordered Locus Names:RHOM_07865 EMBL AEN96686.1
OrganismRoseburia hominis (strain DSM 16839 / NCIMB 14029 / A2-183) [Complete proteome] [HAMAP]
Taxonomic identifier585394 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Roseburia ›

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding75 – 773NADP By similarity PIRSR PIRSR000108-4
Nucleotide binding307 – 3126NADP By similarity PIRSR PIRSR000108-4
Region94 – 1007Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding2501Magnesium or manganese By similarity PIRSR PIRSR000108-3
Metal binding2731Magnesium or manganese By similarity PIRSR PIRSR000108-3
Binding site771Substrate By similarity PIRSR PIRSR000108-2
Binding site821NADP By similarity PIRSR PIRSR000108-4
Binding site1091Substrate By similarity PIRSR PIRSR000108-2
Binding site1321Substrate By similarity PIRSR PIRSR000108-2
Binding site2581NADP By similarity PIRSR PIRSR000108-4
Binding site3251NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
Site1391Critical for catalysis By similarity PIRSR PIRSR000108-1
Site2101Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence LengthMass (Da)Tools
G2T1L3 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 8533DA2B9E600923

FASTA40445,627
        10         20         30         40         50         60 
MEKIKMTTPL VEMDGDEMTR ILWKMIKDEL LYPFIDLKTE YYDLGLEHRN ETDDQVTVDS 

        70         80         90        100        110        120 
ANATKKYGVA VKCATITPNA ARMTEYHLKE MWKSPNGTIR AMLDGTVFRA PIIVKGIEPC 

       130        140        150        160        170        180 
VKNWKKPITI ARHAYGDVYK ASEMKIPGPG KCELVYTAED GTETRELIHN FTGAGVVQGQ 

       190        200        210        220        230        240 
HNLCNSIESF ARSCFNYALD TKQDLWFATK DTISKKYDHT FKDIFQEIYD SEYAQKFKDA 

       250        260        270        280        290        300 
GITYFYTLID DAVARVMKSE GGYIWACKNY DGDVMSDMVS SAFGSLAMMT SVLVTPDGKY 

       310        320        330        340        350        360 
EYEAAHGTVQ RHYYKHLKGE ETSTNSVATI FAWTGALRKR GELDGSHELE EFADKLEKAC 

       370        380        390        400 
VKTIEDGKMT KDLALITTIS NPVVLNSEDF IKAIRSTLEG MLLL

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References

[1]"Bi-directional cross-talk between a Clostridium symbiont, Roseburia hominis, the gut immune system and appetite regulation."
Mulder I.E., Aminov R.I., Travis A.J., Lan A., Gaboriau-Routhiau V., Garden K., Logan E., Delday M., Coutts A.G.P., Grant G., Patterson A.M., Cerf-Bensussan N., Kelly D.
Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16839 / NCIMB 14029 / A2-183.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003040 Genomic DNA. Translation: AEN96686.1.
RefSeqYP_004838618.1. NC_015977.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEN96686; AEN96686; RHOM_07865.
GeneID11146774.
KEGGrho:RHOM_07865.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00031.

Enzyme and pathway databases

BioCycRHOM585394:GHYQ-1557-MONOMER.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2T1L3_ROSHA
AccessionPrimary (citable) accession number: G2T1L3
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: April 3, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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