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Protein

Ribonuclease 3

Gene

rnc

Organism
Roseburia hominis (strain DSM 16839 / NCIMB 14029 / A2-183)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451MagnesiumBy similarity
Active sitei49 – 491Sequence Analysis
Metal bindingi141 – 1411MagnesiumBy similarity
Active sitei144 – 1441By similarity
Metal bindingi144 – 1441MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciRHOM585394:GHYQ-3069-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:RHOM_15125
OrganismiRoseburia hominis (strain DSM 16839 / NCIMB 14029 / A2-183)
Taxonomic identifieri585394 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeRoseburia
ProteomesiUP000008178 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Ribonuclease 3PRO_0000416613Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 155150RNase IIIAdd
BLAST
Domaini198 – 26770DRBM 1Add
BLAST
Domaini285 – 35571DRBM 2Add
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK03685.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 2 hits.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 2 hits.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
PROSITEiPS50137. DS_RBD. 2 hits.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G2SYN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEKEIKFIQ DQIGYTFKNQ ELLVQAFTRR SYSMENGGQD NEVLEFIGDK
60 70 80 90 100
ALDFVVVKQL SEEFGHYSKK YQNWEKWGKT EETGTFISDL DEGELTEIKK
110 120 130 140 150
QLVQKNTLAD AIDNLGIAYY LIMGKGDVEK NIQDSLSVKE DLFEAILGAI
160 170 180 190 200
ALDSNWDIEA LQDSMNVMLN PGELMFDEDV NYVAEIQAWS SANSGNIPLH
210 220 230 240 250
CFYPTSMQGT WYMPRHHMCI YGKAEQDTHF ACEVLIPGID YHFVGYGRSK
260 270 280 290 300
NLARMDASRL AYEYLKDEDM LFSIRDEIDD PNYNDSIGQL EILARRGYFS
310 320 330 340 350
IPQYDFKETH DEDGNPVWNC KCSIKEKDTV TNGRSSSKKD AKKQAAYDML

TFVLEEE
Length:357
Mass (Da):41,045
Last modified:November 16, 2011 - v1
Checksum:i9D9FE4E68BD9B326
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003040 Genomic DNA. Translation: AEN98131.1.
RefSeqiWP_014081094.1. NC_015977.1.
YP_004840063.1. NC_015977.1.

Genome annotation databases

EnsemblBacteriaiAEN98131; AEN98131; RHOM_15125.
KEGGirho:RHOM_15125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003040 Genomic DNA. Translation: AEN98131.1.
RefSeqiWP_014081094.1. NC_015977.1.
YP_004840063.1. NC_015977.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEN98131; AEN98131; RHOM_15125.
KEGGirho:RHOM_15125.

Phylogenomic databases

KOiK03685.

Enzyme and pathway databases

BioCyciRHOM585394:GHYQ-3069-MONOMER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 2 hits.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 2 hits.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
PROSITEiPS50137. DS_RBD. 2 hits.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Bi-directional cross-talk between a Clostridium symbiont, Roseburia hominis, the gut immune system and appetite regulation."
    Mulder I.E., Aminov R.I., Travis A.J., Lan A., Gaboriau-Routhiau V., Garden K., Logan E., Delday M., Coutts A.G.P., Grant G., Patterson A.M., Cerf-Bensussan N., Kelly D.
    Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16839 / NCIMB 14029 / A2-183.

Entry informationi

Entry nameiRNC_ROSHA
AccessioniPrimary (citable) accession number: G2SYN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 16, 2011
Last modified: May 27, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.