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G2SYN4 (RNC_ROSHA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:RHOM_15125
OrganismRoseburia hominis (strain DSM 16839 / NCIMB 14029 / A2-183) [Complete proteome] [HAMAP]
Taxonomic identifier585394 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeRoseburia

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 2 DRBM (double-stranded RNA-binding) domains.

Contains 1 RNase III domain.

Ontologies

Keywords
   Biological processmRNA processing
rRNA processing
tRNA processing
   Cellular componentCytoplasm
   DomainRepeat
   LigandMagnesium
Metal-binding
RNA-binding
rRNA-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease III activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000416613

Regions

Domain6 – 155150RNase III
Domain198 – 26770DRBM 1
Domain285 – 35571DRBM 2

Sites

Active site491 Potential
Active site1441 By similarity
Metal binding451Magnesium By similarity
Metal binding1411Magnesium By similarity
Metal binding1441Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
G2SYN4 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 9D9FE4E68BD9B326

FASTA35741,045
        10         20         30         40         50         60 
MDEKEIKFIQ DQIGYTFKNQ ELLVQAFTRR SYSMENGGQD NEVLEFIGDK ALDFVVVKQL 

        70         80         90        100        110        120 
SEEFGHYSKK YQNWEKWGKT EETGTFISDL DEGELTEIKK QLVQKNTLAD AIDNLGIAYY 

       130        140        150        160        170        180 
LIMGKGDVEK NIQDSLSVKE DLFEAILGAI ALDSNWDIEA LQDSMNVMLN PGELMFDEDV 

       190        200        210        220        230        240 
NYVAEIQAWS SANSGNIPLH CFYPTSMQGT WYMPRHHMCI YGKAEQDTHF ACEVLIPGID 

       250        260        270        280        290        300 
YHFVGYGRSK NLARMDASRL AYEYLKDEDM LFSIRDEIDD PNYNDSIGQL EILARRGYFS 

       310        320        330        340        350 
IPQYDFKETH DEDGNPVWNC KCSIKEKDTV TNGRSSSKKD AKKQAAYDML TFVLEEE 

« Hide

References

[1]"Bi-directional cross-talk between a Clostridium symbiont, Roseburia hominis, the gut immune system and appetite regulation."
Mulder I.E., Aminov R.I., Travis A.J., Lan A., Gaboriau-Routhiau V., Garden K., Logan E., Delday M., Coutts A.G.P., Grant G., Patterson A.M., Cerf-Bensussan N., Kelly D.
Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16839 / NCIMB 14029 / A2-183.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003040 Genomic DNA. Translation: AEN98131.1.
RefSeqYP_004840063.1. NC_015977.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEN98131; AEN98131; RHOM_15125.
GeneID11148543.
KEGGrho:RHOM_15125.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03685.

Enzyme and pathway databases

BioCycRHOM585394:GHYQ-3069-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 2 hits.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 2 hits.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
PROSITEPS50137. DS_RBD. 2 hits.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_ROSHA
AccessionPrimary (citable) accession number: G2SYN4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 16, 2011
Last modified: April 16, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families