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Protein

Acetylornithine aminotransferase

Gene

argD

Organism
Lactobacillus ruminis (strain ATCC 27782 / RF3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (LRC_17890)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine aminotransferase (argD), Acetylornithine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei135 – 1351Pyridoxal phosphate; via carbonyl oxygenUniRule annotation
Binding sitei138 – 1381N2-acetyl-L-ornithineUniRule annotation
Binding sitei299 – 2991Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

AminotransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciLRUM1069534:GJVX-1792-MONOMER.
UniPathwayiUPA00068; UER00109.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylornithine aminotransferaseUniRule annotation (EC:2.6.1.11UniRule annotation)
Short name:
ACOATUniRule annotation
Gene namesi
Name:argDUniRule annotation
Ordered Locus Names:LRC_17180Imported
OrganismiLactobacillus ruminis (strain ATCC 27782 / RF3)Imported
Taxonomic identifieri1069534 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001279 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei270 – 2701N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliG2SS12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni241 – 2444Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotation

Phylogenomic databases

KOiK00823.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_01107. ArgD_aminotrans_3.
InterProiIPR004636. AcOrn/SuccOrn_fam.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G2SS12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLYERSLK VFPPVAGRAT KIGAVSGQGS WLIDESGKKY LDFASGVAVV
60 70 80 90 100
NCGHNHPKVV EAVEKQLHEL VHGGHNVVYY PKYVELAEKL VARVGKPGEY
110 120 130 140 150
KVYFSNSGAE ANEGAVKLAL KATGRPGIIA FNGSFHGRTL LCASMTASSA
160 170 180 190 200
LYRENYEPEL PQVYHADFPD VWNTDLTEQE EIERCLNSVH KIFTNLILPE
210 220 230 240 250
RVACMVIEPV QGEGGYLPAP KRFIAELRKI CDEHGILLIF DEVQSGYGRT
260 270 280 290 300
GYLFAKDYYG VQPDIFTSAK AIANGIPLSA VIGKTEYMDK WLAGSHGGTF
310 320 330 340 350
GGNPLACAAG CAVLDVIDED FLEDVRKKGA FIRERLEDLK VTHSNVISSI
360 370 380 390 400
RGIGMMNGVE FRKDGKPLPK IVSKVQEIAL SKGLILLNCG TFHNVIRLIP
410 420
PLTATKEEIE QAIGIIDECL KEIEG
Length:425
Mass (Da):46,524
Last modified:November 16, 2011 - v1
Checksum:iA801693CFBC42698
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003032 Genomic DNA. Translation: AEN78960.1.
RefSeqiWP_014074101.1. NC_015975.1.

Genome annotation databases

EnsemblBacteriaiAEN78960; AEN78960; LRC_17180.
KEGGilrm:LRC_17180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003032 Genomic DNA. Translation: AEN78960.1.
RefSeqiWP_014074101.1. NC_015975.1.

3D structure databases

ProteinModelPortaliG2SS12.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEN78960; AEN78960; LRC_17180.
KEGGilrm:LRC_17180.

Phylogenomic databases

KOiK00823.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00109.
BioCyciLRUM1069534:GJVX-1792-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_01107. ArgD_aminotrans_3.
InterProiIPR004636. AcOrn/SuccOrn_fam.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequences and comparative genomics of two Lactobacillus ruminis strains from the bovine and human intestinal tracts."
    Forde B.M., Neville B.A., O'Donnell M.M., Riboulet-Bisson E., Claesson M.J., Coghlan A., Ross R.P., O'Toole P.W.
    Microb. Cell Fact. 10:S13-S13(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27782 / RF3Imported.

Entry informationi

Entry nameiG2SS12_LACRR
AccessioniPrimary (citable) accession number: G2SS12
Entry historyi
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: July 22, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.