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G2SQ61 (G2SQ61_LACRR) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:LRC_13340 EMBL AEN78587.1
OrganismLactobacillus ruminis (strain ATCC 27782 / RF3) [Complete proteome] [HAMAP] EMBL AEN78587.1
Taxonomic identifier1069534 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 347207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding171 – 22656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3011Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G2SQ61 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 23DF8AAB13716E13

FASTA38242,810
        10         20         30         40         50         60 
MSEKKLHIAL LFGGNSSEHD VSKRSAHTVF DALDKDKYEV SVFMFTKQGF LLGNKDSMRI 

        70         80         90        100        110        120 
FDGEPEDAVV AEAIKDVDFS NPLANIQSLS EVKDIDLFIP VIHGNMGEDG TVQSLFRLLN 

       130        140        150        160        170        180 
KPYIGSGIAS SAVSFDKDLT KQLLTYHGIR NTKYVVVTPE NQSEYTYEKC AAELGETLFV 

       190        200        210        220        230        240 
KPARQGSSVG IYKVSNKEEF DKAIQDGFHY DFKVLVEETI ANPREVECSV LGNRDIKASK 

       250        260        270        280        290        300 
LGAIRIPAED AFYDYNNKFV DASGVVFEIP VELPEKLTKE IQQMSLDAFR VLGNRGLARM 

       310        320        330        340        350        360 
DFLVDENDVP YFGEVNTLPG FTNISLYPQL WAVSGISYKD LLDRLIQLAF DEFEDNAKLH 

       370        380 
YDFVELGSEK LGKGLLTDED KK 

« Hide

References

[1]"Genome sequences and comparative genomics of two Lactobacillus ruminis strains from the bovine and human intestinal tracts."
Forde B.M., Neville B.A., O'Donnell M.M., Riboulet-Bisson E., Claesson M.J., Coghlan A., Ross R.P., O'Toole P.W.
Microb. Cell Fact. 10:S13-S13(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27782 / RF3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003032 Genomic DNA. Translation: AEN78587.1.
RefSeqYP_004832523.1. NC_015975.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEN78587; AEN78587; LRC_13340.
GeneID11141183.
KEGGlrm:LRC_13340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycLRUM1069534:GJVX-1385-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2SQ61_LACRR
AccessionPrimary (citable) accession number: G2SQ61
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: June 11, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)