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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Enterobacter asburiae (strain LF7a)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei18 – 181S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei20 – 201S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei45 – 451S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei66 – 661S-adenosyl-L-methionineUniRule annotation
Binding sitei91 – 911S-adenosyl-L-methionineUniRule annotation
Binding sitei113 – 1131S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

rRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

RNA-bindingUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciEASB640513:GKDM-671-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotationSAAS annotation (EC:2.1.1.182UniRule annotationSAAS annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:Entas_0653Imported
OrganismiEnterobacter asburiae (strain LF7a)Imported
Taxonomic identifieri640513 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000008527 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

KOiK02528.
OMAiDHVMVEI.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G2S5R4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRVHQGHL ARKRFGQNFL NDQFVIESIV SAINPQKGQA MVEIGPGLAA
60 70 80 90 100
LTEPVGERLD ELTVIELDRD LAARLQTHPF LGPKLTIYQQ DAMTMNFGEL
110 120 130 140 150
SEKMGQPLRV FGNLPYNIST PLMFHLFSYT DAIADMHFML QKEVVNRMVA
160 170 180 190 200
GPNSKAYGRL SVMAQYFCNV IPVLEVPPSA FTPPPKVDSA VVRLVPHKVK
210 220 230 240 250
PYPVKELRVL SRITTEAFNQ RRKTIRNSLS NSFSVEVLAE LGIDPAMRAE
260 270
NISVEQYCKL ANYISENAPP KES
Length:273
Mass (Da):30,598
Last modified:November 16, 2011 - v1
Checksum:i8EAA07C1D86679B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003026 Genomic DNA. Translation: AEN63404.1.
RefSeqiWP_014068864.1. NC_015968.1.
YP_004827189.1. NC_015968.1.

Genome annotation databases

EnsemblBacteriaiAEN63404; AEN63404; Entas_0653.
KEGGieas:Entas_0653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003026 Genomic DNA. Translation: AEN63404.1.
RefSeqiWP_014068864.1. NC_015968.1.
YP_004827189.1. NC_015968.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEN63404; AEN63404; Entas_0653.
KEGGieas:Entas_0653.

Phylogenomic databases

KOiK02528.
OMAiDHVMVEI.

Enzyme and pathway databases

BioCyciEASB640513:GKDM-671-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Enterobacter asburiae LF7a."
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., van der Lelie D., Woyke T.
    Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LF7aImported.

Entry informationi

Entry nameiG2S5R4_ENTAL
AccessioniPrimary (citable) accession number: G2S5R4
Entry historyi
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: May 27, 2015
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.