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G2S5R4 (G2S5R4_ENTAL) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
EC=2.1.1.182 HAMAP-Rule MF_00607
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase HAMAP-Rule MF_00607
16S rRNA dimethyladenosine transferase HAMAP-Rule MF_00607
16S rRNA dimethylase HAMAP-Rule MF_00607
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase HAMAP-Rule MF_00607
Gene names
Name:rsmA HAMAP-Rule MF_00607
Synonyms:ksgA HAMAP-Rule MF_00607
Ordered Locus Names:Entas_0653 EMBL AEN63404.1
OrganismEnterobacter asburiae (strain LF7a) [Complete proteome] [HAMAP]
Taxonomic identifier640513 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex ›

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607 SAAS SAAS023165

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00607 SAAS SAAS023165.

Sequence similarities

Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily. HAMAP-Rule MF_00607

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site181S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site201S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP-Rule MF_00607
Binding site451S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site661S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site911S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site1131S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607

Sequences

Sequence LengthMass (Da)Tools
G2S5R4 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 8EAA07C1D86679B5

FASTA27330,598
        10         20         30         40         50         60 
MTNRVHQGHL ARKRFGQNFL NDQFVIESIV SAINPQKGQA MVEIGPGLAA LTEPVGERLD 

        70         80         90        100        110        120 
ELTVIELDRD LAARLQTHPF LGPKLTIYQQ DAMTMNFGEL SEKMGQPLRV FGNLPYNIST 

       130        140        150        160        170        180 
PLMFHLFSYT DAIADMHFML QKEVVNRMVA GPNSKAYGRL SVMAQYFCNV IPVLEVPPSA 

       190        200        210        220        230        240 
FTPPPKVDSA VVRLVPHKVK PYPVKELRVL SRITTEAFNQ RRKTIRNSLS NSFSVEVLAE 

       250        260        270 
LGIDPAMRAE NISVEQYCKL ANYISENAPP KES

« Hide

References

[1]"Complete sequence of chromosome of Enterobacter asburiae LF7a."
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., van der Lelie D., Woyke T.
Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LF7a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003026 Genomic DNA. Translation: AEN63404.1.
RefSeqYP_004827189.1. NC_015968.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEN63404; AEN63404; Entas_0653.
GeneID11108982.
KEGGeas:Entas_0653.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK02528.
OMAPVPNVDS.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2S5R4_ENTAL
AccessionPrimary (citable) accession number: G2S5R4
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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