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G2S4J2

- G2S4J2_ENTAL

UniProt

G2S4J2 - G2S4J2_ENTAL

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Protein
Fatty acid oxidation complex subunit alpha
Gene
fadB, Entas_4232
Organism
Enterobacter asburiae (strain LF7a)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate By similarity.UniRule annotationSAAS annotations

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotationSAAS annotations
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotationSAAS annotations
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotationSAAS annotations
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei116 – 1161For enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities By similarityUniRule annotation
Sitei119 – 1191Important for catalytic activity By similarityUniRule annotation
Sitei139 – 1391Important for catalytic activity By similarityUniRule annotation
Binding sitei296 – 2961Substrate By similarityUniRule annotation
Binding sitei324 – 3241NAD; via amide nitrogen By similarityUniRule annotation
Binding sitei343 – 3431NAD By similarityUniRule annotation
Binding sitei407 – 4071NAD By similarityUniRule annotation
Active sitei450 – 4501For 3-hydroxyacyl-CoA dehydrogenase activity By similarityUniRule annotation
Binding sitei453 – 4531NAD By similarityUniRule annotation
Binding sitei500 – 5001Substrate By similarityUniRule annotation
Binding sitei660 – 6601Substrate By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi400 – 4023NAD By similarityUniRule annotation
Nucleotide bindingi427 – 4293NAD By similarityUniRule annotation

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. coenzyme binding Source: InterPro
  4. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB-HAMAP
  5. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotations, LyaseUniRule annotationSAAS annotations, OxidoreductaseUniRule annotationSAAS annotations

Keywords - Biological processi

Fatty acid metabolismUniRule annotationSAAS annotations, Lipid degradationUniRule annotationSAAS annotations, Lipid metabolism

Keywords - Ligandi

NADUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciEASB640513:GKDM-4327-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:Entas_4232Imported
OrganismiEnterobacter asburiae (strain LF7a)Imported
Taxonomic identifieri640513 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
ProteomesiUP000008527: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. fatty acid beta-oxidation multienzyme complex Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA) By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliG2S4J2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 189189Enoyl-CoA hydratase/isomerase By similarityUniRule annotation
Add
BLAST
Regioni311 – 7294193-hydroxyacyl-CoA dehydrogenase By similarityUniRule annotation
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation

Phylogenomic databases

KOiK01825.
OMAiAKGMVMQ.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPiMF_01621. FadB.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G2S4J2-1 [UniParc]FASTAAdd to Basket

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MLYKGDTLYL NWLEDGIAEL VFDAPGSVNK LDTATVASLG QALDVLEKQS    50
DLKGLLLRSN KAAFIVGADI TEFLSLFLVP EEQLSQWLHF ANSVFNRLED 100
LPVPTISAVN GYALGGGCEC VLATDYRLAT PDLRIGLPET KLGIMPGFGG 150
SVRMPRMLGA DSALEIIAAG KDVGAEQAQK IGLVDGVVKP EKLVEGAIAI 200
LRQAINGDLD WKAKRQPKLE PLKLSKIEAA MSFTIAKGMV MQTAGKHYPA 250
PITAVKTIEA AARFGREEAL KLENQSFVPL AHTNEARALV GIFLNDQFVK 300
GKAKQLTKNV ETPKHAAVLG AGIMGGGIAY QSAWKGVPVV MKDISEKSLT 350
LGMTEAAKLL NKQLERGKID GLKLAGVIST IQPTLEYSGF DRVDVVVEAV 400
VENPKVKKAV LAETEDKVRQ DTVLASNTST IPISELASVL KRPENFCGMH 450
FFNPVHRMPL VEVIRGEKTS DDTIAKVVAW ASKMGKTPIV VNDCPGFFVN 500
RVLFPYFAGF SQLLRDGADF RKVDKVMEKQ FGWPMGPAYL LDVVGIDTAH 550
HAQAVMAAGF PQRMSKDYRD AIDAMFDANR FGQKNGLGFW RYKEDSKGKP 600
KKEEDAAVES LLADVSAPKR DFTDEEIIAR MMIPMVNEVV RCLEEGIIAS 650
PAEADMALVY GLGFPPFHGG AFRWLDTLGS AKYLDMAQQY QHLGPLYAVP 700
EGLRNKARHN EPYYPAVEPA RPVGELKTA 729
Length:729
Mass (Da):79,503
Last modified:November 16, 2011 - v1
Checksum:iCF0478D721DCC6B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003026 Genomic DNA. Translation: AEN66937.1.
RefSeqiWP_014072242.1. NC_015968.1.
YP_004830722.1. NC_015968.1.

Genome annotation databases

EnsemblBacteriaiAEN66937; AEN66937; Entas_4232.
GeneIDi11110693.
KEGGieas:Entas_4232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003026 Genomic DNA. Translation: AEN66937.1 .
RefSeqi WP_014072242.1. NC_015968.1.
YP_004830722.1. NC_015968.1.

3D structure databases

ProteinModelPortali G2S4J2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEN66937 ; AEN66937 ; Entas_4232 .
GeneIDi 11110693.
KEGGi eas:Entas_4232.

Phylogenomic databases

KOi K01825.
OMAi AKGMVMQ.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci EASB640513:GKDM-4327-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 2 hits.
HAMAPi MF_01621. FadB.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02437. FadB. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Enterobacter asburiae LF7a."
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., van der Lelie D., Woyke T.
    Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LF7a.

Entry informationi

Entry nameiG2S4J2_ENTAL
AccessioniPrimary (citable) accession number: G2S4J2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: September 3, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotations

External Data

Dasty 3

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