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G2S4J2

- G2S4J2_ENTAL

UniProt

G2S4J2 - G2S4J2_ENTAL

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Enterobacter asburiae (strain LF7a)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 19 (01 Oct 2014)
      Sequence version 1 (16 Nov 2011)
      Previous versions | rss
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    Functioni

    Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotationSAAS annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotationSAAS annotation
    (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotationSAAS annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotationSAAS annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei119 – 1191Important for catalytic activityUniRule annotation
    Sitei139 – 1391Important for catalytic activityUniRule annotation
    Binding sitei296 – 2961SubstrateUniRule annotation
    Binding sitei324 – 3241NAD; via amide nitrogenUniRule annotation
    Binding sitei343 – 3431NADUniRule annotation
    Binding sitei407 – 4071NADUniRule annotation
    Active sitei450 – 4501For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation
    Binding sitei453 – 4531NADUniRule annotation
    Binding sitei500 – 5001SubstrateUniRule annotation
    Binding sitei660 – 6601SubstrateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi400 – 4023NADUniRule annotation
    Nucleotide bindingi427 – 4293NADUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. coenzyme binding Source: InterPro
    4. dodecenoyl-CoA delta-isomerase activity Source: UniProtKB-HAMAP
    5. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    IsomeraseUniRule annotationSAAS annotation, LyaseUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Fatty acid metabolismUniRule annotationSAAS annotation, Lipid degradationUniRule annotationSAAS annotation, Lipid metabolism

    Keywords - Ligandi

    NADUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciEASB640513:GKDM-4327-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Gene namesi
    Name:fadBUniRule annotation
    Ordered Locus Names:Entas_4232Imported
    OrganismiEnterobacter asburiae (strain LF7a)Imported
    Taxonomic identifieri640513 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
    ProteomesiUP000008527: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. fatty acid beta-oxidation multienzyme complex Source: InterPro

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliG2S4J2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 189189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd
    BLAST
    Regioni311 – 7294193-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation
    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation

    Phylogenomic databases

    KOiK01825.
    OMAiAKGMVMQ.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 2 hits.
    HAMAPiMF_01621. FadB.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR012799. FadB.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02437. FadB. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G2S4J2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLYKGDTLYL NWLEDGIAEL VFDAPGSVNK LDTATVASLG QALDVLEKQS    50
    DLKGLLLRSN KAAFIVGADI TEFLSLFLVP EEQLSQWLHF ANSVFNRLED 100
    LPVPTISAVN GYALGGGCEC VLATDYRLAT PDLRIGLPET KLGIMPGFGG 150
    SVRMPRMLGA DSALEIIAAG KDVGAEQAQK IGLVDGVVKP EKLVEGAIAI 200
    LRQAINGDLD WKAKRQPKLE PLKLSKIEAA MSFTIAKGMV MQTAGKHYPA 250
    PITAVKTIEA AARFGREEAL KLENQSFVPL AHTNEARALV GIFLNDQFVK 300
    GKAKQLTKNV ETPKHAAVLG AGIMGGGIAY QSAWKGVPVV MKDISEKSLT 350
    LGMTEAAKLL NKQLERGKID GLKLAGVIST IQPTLEYSGF DRVDVVVEAV 400
    VENPKVKKAV LAETEDKVRQ DTVLASNTST IPISELASVL KRPENFCGMH 450
    FFNPVHRMPL VEVIRGEKTS DDTIAKVVAW ASKMGKTPIV VNDCPGFFVN 500
    RVLFPYFAGF SQLLRDGADF RKVDKVMEKQ FGWPMGPAYL LDVVGIDTAH 550
    HAQAVMAAGF PQRMSKDYRD AIDAMFDANR FGQKNGLGFW RYKEDSKGKP 600
    KKEEDAAVES LLADVSAPKR DFTDEEIIAR MMIPMVNEVV RCLEEGIIAS 650
    PAEADMALVY GLGFPPFHGG AFRWLDTLGS AKYLDMAQQY QHLGPLYAVP 700
    EGLRNKARHN EPYYPAVEPA RPVGELKTA 729
    Length:729
    Mass (Da):79,503
    Last modified:November 16, 2011 - v1
    Checksum:iCF0478D721DCC6B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003026 Genomic DNA. Translation: AEN66937.1.
    RefSeqiWP_014072242.1. NC_015968.1.
    YP_004830722.1. NC_015968.1.

    Genome annotation databases

    EnsemblBacteriaiAEN66937; AEN66937; Entas_4232.
    GeneIDi11110693.
    KEGGieas:Entas_4232.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003026 Genomic DNA. Translation: AEN66937.1 .
    RefSeqi WP_014072242.1. NC_015968.1.
    YP_004830722.1. NC_015968.1.

    3D structure databases

    ProteinModelPortali G2S4J2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEN66937 ; AEN66937 ; Entas_4232 .
    GeneIDi 11110693.
    KEGGi eas:Entas_4232.

    Phylogenomic databases

    KOi K01825.
    OMAi AKGMVMQ.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci EASB640513:GKDM-4327-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 2 hits.
    HAMAPi MF_01621. FadB.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR018376. Enoyl-CoA_hyd/isom_CS.
    IPR012799. FadB.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02437. FadB. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    PS00166. ENOYL_COA_HYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Enterobacter asburiae LF7a."
      Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., van der Lelie D., Woyke T.
      Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LF7aImported.

    Entry informationi

    Entry nameiG2S4J2_ENTAL
    AccessioniPrimary (citable) accession number: G2S4J2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 16, 2011
    Last sequence update: November 16, 2011
    Last modified: October 1, 2014
    This is version 19 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation

    External Data

    Dasty 3