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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Enterobacter asburiae (strain LF7a)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotationSAAS annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotationSAAS annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotationSAAS annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotationSAAS annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotationSAAS annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei139Important for catalytic activityUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei324NAD; via amide nitrogenUniRule annotation1
Binding sitei343NADUniRule annotation1
Binding sitei407NADUniRule annotation1
Binding sitei429NADUniRule annotation1
Active sitei450For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei453NADUniRule annotation1
Binding sitei500SubstrateUniRule annotation1
Binding sitei660SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi400 – 402NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotation, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation
Biological processFatty acid metabolismUniRule annotationSAAS annotation, Lipid degradationUniRule annotationSAAS annotation, Lipid metabolism
LigandNADUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:Entas_4232Imported
OrganismiEnterobacter asburiae (strain LF7a)Imported
Taxonomic identifieri640513 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
Proteomesi
  • UP000008527 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi640513.Entas_4232

Structurei

3D structure databases

ProteinModelPortaliG2S4J2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini316 – 4943HCDH_NInterPro annotationAdd BLAST179
Domaini496 – 5923HCDHInterPro annotationAdd BLAST97
Domaini627 – 6913HCDHInterPro annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST189
Regioni311 – 7293-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST419

Sequence similaritiesi

In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
KOiK01825
OMAiIDLCMIL
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01621 FadB, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR012799 FadB
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02437 FadB, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

G2S4J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYKGDTLYL NWLEDGIAEL VFDAPGSVNK LDTATVASLG QALDVLEKQS
60 70 80 90 100
DLKGLLLRSN KAAFIVGADI TEFLSLFLVP EEQLSQWLHF ANSVFNRLED
110 120 130 140 150
LPVPTISAVN GYALGGGCEC VLATDYRLAT PDLRIGLPET KLGIMPGFGG
160 170 180 190 200
SVRMPRMLGA DSALEIIAAG KDVGAEQAQK IGLVDGVVKP EKLVEGAIAI
210 220 230 240 250
LRQAINGDLD WKAKRQPKLE PLKLSKIEAA MSFTIAKGMV MQTAGKHYPA
260 270 280 290 300
PITAVKTIEA AARFGREEAL KLENQSFVPL AHTNEARALV GIFLNDQFVK
310 320 330 340 350
GKAKQLTKNV ETPKHAAVLG AGIMGGGIAY QSAWKGVPVV MKDISEKSLT
360 370 380 390 400
LGMTEAAKLL NKQLERGKID GLKLAGVIST IQPTLEYSGF DRVDVVVEAV
410 420 430 440 450
VENPKVKKAV LAETEDKVRQ DTVLASNTST IPISELASVL KRPENFCGMH
460 470 480 490 500
FFNPVHRMPL VEVIRGEKTS DDTIAKVVAW ASKMGKTPIV VNDCPGFFVN
510 520 530 540 550
RVLFPYFAGF SQLLRDGADF RKVDKVMEKQ FGWPMGPAYL LDVVGIDTAH
560 570 580 590 600
HAQAVMAAGF PQRMSKDYRD AIDAMFDANR FGQKNGLGFW RYKEDSKGKP
610 620 630 640 650
KKEEDAAVES LLADVSAPKR DFTDEEIIAR MMIPMVNEVV RCLEEGIIAS
660 670 680 690 700
PAEADMALVY GLGFPPFHGG AFRWLDTLGS AKYLDMAQQY QHLGPLYAVP
710 720
EGLRNKARHN EPYYPAVEPA RPVGELKTA
Length:729
Mass (Da):79,503
Last modified:November 16, 2011 - v1
Checksum:iCF0478D721DCC6B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003026 Genomic DNA Translation: AEN66937.1
RefSeqiWP_014072242.1, NC_015968.1

Genome annotation databases

EnsemblBacteriaiAEN66937; AEN66937; Entas_4232
KEGGieas:Entas_4232

Similar proteinsi

Entry informationi

Entry nameiG2S4J2_ENTAL
AccessioniPrimary (citable) accession number: G2S4J2
Entry historyiIntegrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: April 25, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health