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G2PT53 (G2PT53_9FIRM) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP-Rule MF_00156
EC=2.1.2.11 HAMAP-Rule MF_00156
Alternative name(s):
Ketopantoate hydroxymethyltransferase HAMAP-Rule MF_00156
Gene names
Name:panB HAMAP-Rule MF_00156
ORF Names:Calla_1609 EMBL AEM74212.1
OrganismCaldicellulosiruptor lactoaceticus 6A EMBL AEM74212.1
Taxonomic identifier632516 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor ›

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP-Rule MF_00156 SAAS SAAS003700

Catalytic activity

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP-Rule MF_00156 SAAS SAAS003700

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00156 SAAS SAAS003700

Pathway

Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP-Rule MF_00156 SAAS SAAS003700

Subunit structure

Homodecamer; pentamer of dimers By similarity. HAMAP-Rule MF_00156 SAAS SAAS003700

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00156 SAAS SAAS003700.

Sequence similarities

Belongs to the PanB family. HAMAP-Rule MF_00156

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region45 – 462Alpha-ketoisovalerate binding By similarity HAMAP-Rule MF_00156

Sites

Active site1821Proton acceptor By similarity HAMAP-Rule MF_00156
Metal binding451Magnesium By similarity HAMAP-Rule MF_00156
Metal binding841Magnesium By similarity HAMAP-Rule MF_00156
Metal binding1151Magnesium By similarity HAMAP-Rule MF_00156
Binding site841Alpha-ketoisovalerate By similarity HAMAP-Rule MF_00156
Binding site1131Alpha-ketoisovalerate By similarity HAMAP-Rule MF_00156

Sequences

Sequence LengthMass (Da)Tools
G2PT53 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: A664846FBE420A12

FASTA26429,269
        10         20         30         40         50         60 
MNKVTTKTLF EKKQKGEKIT MLTAYDYTFA KIFDSCMVDI LLVGDSLGMV ILGYDSTIPV 

        70         80         90        100        110        120 
TMDDMEHHVR AVSRGAKYSM VVADMPFLSY HTTTEDAVKN AGRLIRAGAY AVKMEGCDDV 

       130        140        150        160        170        180 
YDKIEAVIKA QIPVMGHLGL TPQSVNIFGG YDLRAKEEAE AKKLVEDAKK LEKAGVFAIV 

       190        200        210        220        230        240 
LEKVPAHVAK QVQESVKVPV IGIGAGPYCD GQVLVCYDML GMYEDFKPKF VKRYAEVGNI 

       250        260 
IKDAVTRYIE EVKKGEFPGK EHSY

« Hide

References

[1]"Complete sequence of Caldicellulosiruptor lactoaceticus 6A."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Blumer-Schuette S.E., Kelly R.M., Woyke T.
Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 6A EMBL AEM74212.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003001 Genomic DNA. Translation: AEM74212.1.
RefSeqYP_004799189.1. NC_015949.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEM74212; AEM74212; Calla_1609.
GeneID11105653.
KEGGclc:Calla_1609.

Phylogenomic databases

KOK00606.

Enzyme and pathway databases

BioCycCLAC632516:GHP3-1645-MONOMER.
UniPathwayUPA00028; UER00003.

Family and domain databases

Gene3D3.20.20.60. 1 hit.
HAMAPMF_00156. PanB.
InterProIPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERPTHR20881. PTHR20881. 1 hit.
PfamPF02548. Pantoate_transf. 1 hit.
[Graphical view]
PIRSFPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMSSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMsTIGR00222. panB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameG2PT53_9FIRM
AccessionPrimary (citable) accession number: G2PT53
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: May 29, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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