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G2NNB6 (G2NNB6_9ACTO) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Probable DNA ligase HAMAP-Rule MF_00407

EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:lig HAMAP-Rule MF_00407
ORF Names:SACTE_0610 EMBL AEN08547.1
OrganismStreptomyces sp. SirexAA-E [Complete proteome] EMBL AEN08547.1
Taxonomic identifier862751 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). RuleBase RU000617 HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2101N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2081ATP By similarity HAMAP-Rule MF_00407
Binding site2151ATP By similarity HAMAP-Rule MF_00407
Binding site2301ATP By similarity HAMAP-Rule MF_00407
Binding site2591ATP By similarity HAMAP-Rule MF_00407
Binding site2991ATP By similarity HAMAP-Rule MF_00407
Binding site3771ATP By similarity HAMAP-Rule MF_00407
Binding site3831ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
G2NNB6 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 4362F0B5505FDDB3

FASTA51154,502
        10         20         30         40         50         60 
MLLARLAHVS LEVAATSARN RKVALLAGLF RETGPDDVPT VIPYLAGRLP QGRLGIGWSA 

        70         80         90        100        110        120 
LSDPPRPADT PVLTVAEAHA ELTAVGALSG PGSQAARKER LRRLFAAATA DEQHFLRGLL 

       130        140        150        160        170        180 
TGELRQGALE ALAADALAAA TGAPPADVRR AVMLAGSLQS VARALLADGP EALAAFRLTV 

       190        200        210        220        230        240 
GRPVLPMLAH TAGSVTEAVD KLGPCAVEEK LDGIRVQVHR DGAGVRIYTR TLDDITDRLP 

       250        260        270        280        290        300 
EPATAVARFP GERFVLDGEV IALGPDGRPR PFQETAARVG SRRDVATAAA AVPVVAVFFD 

       310        320        330        340        350        360 
VLALDGRELI DLPFTERHTA LDTLVPEPMR VRRVTVTDPR DPAARRAAEE FFEAALGRGH 

       370        380        390        400        410        420 
EGVVAKGASA PYSAGRRGAS WLKVKPVHTL DLVVLAAEWG HGRRTGKLSN LHLGARRPDG 

       430        440        450        460        470        480 
TFAMLGKTFK GLTDTMLAWQ TERLLELSTG DDGHVVTVRP ELVVEIAYDG LQDSTRYPAG 

       490        500        510 
VTLRFARVLG YREDKPPEEA DTVDTVLSRR A 

« Hide

References

[1]"Complete sequence of Streptomyces sp. SirexAA-E."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Adams A. expand/collapse author list , Raffa K., Adams S., Book A., Currie C., Woyke T.
Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: SirexAA-E EMBL AEN08547.1.
[2]"Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed beta-Mannanase from Cellulolytic Streptomyces sp. SirexAA-E."
Takasuka T.E., Acheson J.F., Bianchetti C.M., Prom B.M., Bergeman L.F., Book A.J., Currie C.R., Fox B.G.
PLoS ONE 9:E94166-E94166(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: SirexAA-E EMBL AEN08547.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002993 Genomic DNA. Translation: AEN08547.1.
RefSeqYP_004801087.1. NC_015953.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEN08547; AEN08547; SACTE_0610.
GeneID11102866.
KEGGssx:SACTE_0610.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01971.

Enzyme and pathway databases

BioCycSSP862751:GHMW-611-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2NNB6_9ACTO
AccessionPrimary (citable) accession number: G2NNB6
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: June 11, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)