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G2NMC1

- G2NMC1_9ACTO

UniProt

G2NMC1 - G2NMC1_9ACTO

Protein

Proline--tRNA ligase

Gene

proS

Organism
Streptomyces sp. SirexAA-E
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (16 Nov 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotationSAAS annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSSP862751:GHMW-4951-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    ORF Names:SACTE_4873Imported
    OrganismiStreptomyces sp. SirexAA-EImported
    Taxonomic identifieri862751 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
    ProteomesiUP000001397: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01881.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G2NMC1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQVQRMSRL MIKTLRDDPA DAETLNHKLL VRAGYVRRTA AGLWSWLPLG    50
    KKVLENVSRV VREEMDAIGG QEVLLPALLP KEPYEASGRY DEYGDLLFRL 100
    RDRKGAEYLL GPTHEEIFTQ VVKDMCSSYK DLPVILYQIQ TKYRDEARPR 150
    AGVLRGREFQ MKDSYSFDTT DEGLAESYQL HRAAYIRIFE RLGLDHRIVS 200
    AVSGAMGGSA SEEFLAPAPA GEDTFVDCPN CDYAANTEAV TYVATPAEAP 250
    AAGPVEELDT PDTPTIESLA DFLGVPASAT LKNLLVKVDG EIVAVGVPGD 300
    REVDLGKLGE HLAPAEVELV TAEDFVGRPD LVRGYVGPQG LEKVRYIADP 350
    RIAAGTSWIT GANKEGVHAR NVVCGRDFEV DQYLDVVVVE PGDPCPRCGT 400
    GLQVDRAIEI GHIFQLGRKY ADIFSLDVLG QQGKPVRVTM GSYGIGVSRA 450
    VAALAEQTAD DKGLCWPREV APADVHVVAA GKALQTELAL DVSEKLGAAG 500
    LRVIVDDRAG VSPGVKFTDS ELIGVPKILV AGRRSAEGVL ELKDRRTGER 550
    EELTVDEALA RLTDQG 566
    Length:566
    Mass (Da):61,437
    Last modified:November 16, 2011 - v1
    Checksum:iBB688E9E83C5D2ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002993 Genomic DNA. Translation: AEN12699.1.
    RefSeqiYP_004805239.1. NC_015953.1.

    Genome annotation databases

    EnsemblBacteriaiAEN12699; AEN12699; SACTE_4873.
    GeneIDi11103804.
    KEGGissx:SACTE_4873.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002993 Genomic DNA. Translation: AEN12699.1 .
    RefSeqi YP_004805239.1. NC_015953.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEN12699 ; AEN12699 ; SACTE_4873 .
    GeneIDi 11103804.
    KEGGi ssx:SACTE_4873.

    Phylogenomic databases

    KOi K01881.

    Enzyme and pathway databases

    BioCyci SSP862751:GHMW-4951-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed beta-Mannanase from Cellulolytic Streptomyces sp. SirexAA-E."
      Takasuka T.E., Acheson J.F., Bianchetti C.M., Prom B.M., Bergeman L.F., Book A.J., Currie C.R., Fox B.G.
      PLoS ONE 9:E94166-E94166(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SirexAA-EImported.

    Entry informationi

    Entry nameiG2NMC1_9ACTO
    AccessioniPrimary (citable) accession number: G2NMC1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 16, 2011
    Last sequence update: November 16, 2011
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3