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G2N473 (G2N473_MYCTX) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AEN01446.1
ORF Names:MTCTRI2_3040 EMBL AEN01446.1
OrganismMycobacterium tuberculosis CTRI-2 [Complete proteome] EMBL AEN01446.1
Taxonomic identifier707235 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain156 – 363208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding184 – 23956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3181Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3301Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3301Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3321Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G2N473 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: ED7938E8B7526B53

FASTA37339,680
        10         20         30         40         50         60 
MSANDRRDRR VRVAVVFGGR SNEHAISCVS AGSILRNLDS RRFDVIAVGI TPAGSWVLTD 

        70         80         90        100        110        120 
ANPDALTITN RELPQVKSGS GTELALPADP RRGGQLVSLP PGAGEVLESV DVVFPVLHGP 

       130        140        150        160        170        180 
YGEDGTIQGL LELAGVPYVG AGVLASAVGM DKEFTKKLLA ADGLPVGAYA VLRPPRSTLH 

       190        200        210        220        230        240 
RQECERLGLP VFVKPARGGS SIGVSRVSSW DQLPAAVARA RRHDPKVIVE AAISGRELEC 

       250        260        270        280        290        300 
GVLEMPDGTL EASTLGEIRV AGVRGREDSF YDFATKYLDD AAELDVPAKV DDQVAEAIRQ 

       310        320        330        340        350        360 
LAIRAFAAID CRGLARVDFF LTDDGPVINE INTMPGFTTI SMYPRMWAAS GVDYPTLLAT 

       370 
MIETALARGV GLH 

« Hide

References

[1]"The Complete Genome Sequence of Mycobacterium tuberculosis CTRI2."
Shitikov E., Ilina E., Ikryannikova L., Malakhova M., Parfenova T., Afanas'ev M., Kamashev D., Bazaleev N., Ischenko D., Aliper A., Alekseev D., Smirnova T., Chernousova L., Larionova E., Beletsky A., Mardanov A., Ravin N., Skryabin K., Govorun V.
Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: CTRI-2 EMBL AEN01446.1.
[2]"Comparative Genomic Analysis of Mycobacterium tuberculosis Drug Resistant Strains from Russia."
Ilina E.N., Shitikov E.A., Ikryannikova L.N., Alekseev D.G., Kamashev D.E., Malakhova M.V., Parfenova T.V., Afanas'ev M.V., Ischenko D.S., Bazaleev N.A., Smirnova T.G., Larionova E.E., Chernousova L.N., Beletsky A.V., Mardanov A.V., Ravin N.V., Skryabin K.G., Govorun V.M.
PLoS ONE 8:E56577-E56577(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: CTRI-2 EMBL AEN01446.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002992 Genomic DNA. Translation: AEN01446.1.
RefSeqYP_005918067.1. NC_017524.1.

3D structure databases

ProteinModelPortalG2N473.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEN01446; AEN01446; MTCTRI2_3040.
GeneID12508207.
KEGGmto:MTCTRI2_3040.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycMTUB707235:GLGU-3039-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2N473_MYCTX
AccessionPrimary (citable) accession number: G2N473
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: June 11, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)