G2MME4 (G2MME4_9ARCH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase HAMAP-Rule MF_00177
EC=6.1.1.6 HAMAP-Rule MF_00177

Alternative name(s):
Lysyl-tRNA synthetase HAMAP-Rule MF_00177

Gene names

Name:	lysS HAMAP-Rule MF_00177
ORF Names:	Halar_2699 EMBL AEN06343.1

Organism

halophilic archaeon DL31 EMBL AEN06343.1

Taxonomic identifier

756883 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › unclassified Archaea › unclassified Archaea (miscellaneous)

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00177
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00177.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. RuleBase RU003489 HAMAP-Rule MF_00177

Ontologies

Keywords
Biological process	Protein biosynthesis RuleBase RU003489 HAMAP-Rule MF_00177
Cellular component	Cytoplasm HAMAP-Rule MF_00177
Ligand	ATP-binding RuleBase RU003489 HAMAP-Rule MF_00177 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase RuleBase RU003489 HAMAP-Rule MF_00177 EMBL AEN06343.1 Ligase
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	41 – 49	9	"HIGH" region By similarity HAMAP-Rule MF_00177
	Motif	307 – 311	5	"KMSKS" region By similarity HAMAP-Rule MF_00177

Sequences

	Sequence		Length	Mass (Da)
	G2MME4 [UniParc]. Last modified November 16, 2011. Version 1. Checksum: 020D9BB14C6A1BDB	FASTA	543	60,899
	10 20 30 40 50 60 MSSDDRHGDR SFHAFWADTV ADEIEASEPE EPIVIKGGVS PSGIPHLGHF NEILRGHFVA 70 80 90 100 110 120 EVLRERGHEV RQVFMSDDRD PLRKLPRRLA DADWNVVELG DVDAGALGKN LGKPYTDIPD 130 140 150 160 170 180 PFGECHDSYG EHFADLLSQA ADMVDVPVEV LSNTEMYESG EFEPQIRTIL ENVETAREVL 190 200 210 220 230 240 AGYQNSVDES YSPFRVVCQE CGIVTTDVTV TDLGEGIVSY ACTGMTAGGR DIEGCGHEGE 250 260 270 280 290 300 ATLRDGKLSW RFEWPAQWSL LGVDFEPFGK DHAEGSWPSG EEIMREVLGE EPPVPMTYEW 310 320 330 340 350 360 FTLNGEPLSS SEGNVVTVPE VLELLEPEVL RYFFALDPNK ARDFDVSRLD QLVDDFDRFE 370 380 390 400 410 420 RAHFGDVDDE ELTRVAERAY PFVVDEVRAD RVRLPYTFAA VLGMVDDRHL RLQMAQNEGH 430 440 450 460 470 480 IPEDAPEWVV DDALARVEKA RAWAERCDNA YNYRLQPDLP DAAFDDAVAA ALDELAAFVE 490 500 510 520 530 540 AGNDGEAIQG EIYETAKRHD IEVGDLFAAG YRLFFDQTQG PRLGEFMGEL DREFVVQRLR RDG

References

[1]

"Complete sequence of chromosome of halophilic archaeon DL31." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Dyall-Smith M., Cavicchioli R., Woyke T. Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DL31 EMBL AEN06343.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002988 Genomic DNA. Translation: AEN06343.1.
RefSeq	YP_004808716.1. NC_015954.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEN06343; AEN06343; Halar_2699.
GeneID	11096068.
KEGG	hah:Halar_2699.
Phylogenomic databases
KO	K04566.
Enzyme and pathway databases
BioCyc	HARC756883:GJDQ-2011-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00177. Lys_tRNA_synth_class1.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR002904. Lys-tRNA-ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01921. tRNA-synt_1f. 1 hit. [Graphical view]
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00467. lysS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G2MME4_9ARCH

Accession

Primary (citable) accession number: G2MME4

Entry history

Integrated into UniProtKB/TrEMBL:	November 16, 2011
Last sequence update:	November 16, 2011
Last modified:	May 1, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)