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G2L8L0 (G2L8L0_PSEAI) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AEO76982.1
ORF Names:PAM18_4501 EMBL AEO76982.1
OrganismPseudomonas aeruginosa M18 [Complete proteome] EMBL AEO76982.1
Taxonomic identifier941193 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain117 – 312196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding143 – 19856ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2661Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2791Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2791Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2811Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G2L8L0 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 989DE3B4A6995404

FASTA31934,390
        10         20         30         40         50         60 
MNLCLDSLLN GTQDPKAFGR VAVLFGGKSA EREVSLKSGA MVLQSLLAAG VDAFGIDVGE 

        70         80         90        100        110        120 
DLLQRLVEEK IDRAFIILHG RGGEDGSMQG LLECAGIPYT GSGVLASALA MDKLRTKRVW 

       130        140        150        160        170        180 
LSLGLPTPDY AVLANEDDCR EAAQRLGFPL IVKPAHEGSS IGMAKVGGLD ELIAAWREAA 

       190        200        210        220        230        240 
RYDSQVLVEQ WISGPEFTVA TLRGQVLPAI RLGTPHTFYD YDAKYLASDT RYQVPCGLDE 

       250        260        270        280        290        300 
AKERELKELT ARACDALGIQ GWGRADVMQD AEGRFWLLEV NTAPGMTDHS LVPMAARAAG 

       310 
LDFQQLVLAI LADSREARG 

« Hide

References

[1]"Genomic analysis and temperature-dependent transcriptome profiles of the rhizosphere originating strain Pseudomonas aeruginosa M18."
Wu D.Q., Ye J., Ou H.Y., Wei X., Huang X., He Y.W., Xu Y.
BMC Genomics 12:438-438(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: M18 EMBL AEO76982.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002496 Genomic DNA. Translation: AEO76982.1.
RefSeqYP_005977084.1. NC_017548.1.

3D structure databases

ProteinModelPortalG2L8L0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEO76982; AEO76982; PAM18_4501.
GeneID12568568.
KEGGpaf:PAM18_4501.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPAER941193:GLIQ-4550-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2L8L0_PSEAI
AccessionPrimary (citable) accession number: G2L8L0
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: June 11, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)