G2K4Q7 (ALR_LISM4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Synonyms:	dal
Ordered Locus Names:	LMRG_02310

Organism

Listeria monocytogenes serotype 1/2a (strain 10403S) [Complete proteome] [HAMAP]

Taxonomic identifier

393133 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Alanine racemase HAMAP-Rule MF_01201		PRO_0000418517
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	263	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	310	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	G2K4Q7 [UniParc]. Last modified November 16, 2011. Version 1. Checksum: E3D66B1952DC2959	FASTA	368	41,029
	10 20 30 40 50 60 MVTGWHRPTW IEIDRAAIRE NIKNEQNKLP ESVDLWAVVK ANAYGHGIIE VARTAKEAGA 70 80 90 100 110 120 KGFCVAILDE ALALREAGFQ DDFILVLGAT RKEDANLAAK NHISLTVFRE DWLENLTLEA 130 140 150 160 170 180 TLRIHLKVDS GMGRLGIRTT EEARRIEATS TNDHQLQLEG IYTHFATADQ LETSYFEQQL 190 200 210 220 230 240 AKFQTILTSL KKRPTYVHTA NSAASLLQPQ IGFDAIRFGI SMYGLTPSTE IKTSLPFELK 250 260 270 280 290 300 PALALYTEMV HVKELAPGDS VSYGATYTAT EREWVATLPI GYADGLIRHY SGFHVLVDGE 310 320 330 340 350 360 PAPIIGRVCM DQTIIKLPRE FQTGSKVTII GKDHGNTVTA DDAAQYLDTI NYEVTCLLNE RIPRKYIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pathogenicity and immunogenicity of a Listeria monocytogenes strain that requires D-alanine for growth." Thompson R.J., Bouwer H.G.A., Portnoy D.A., Frankel F.R. Infect. Immun. 66:3552-3561(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 10403S.
[2]	"The genome sequence of Listeria monocytogenes strain 10403S." The Broad Institute Genome Sequencing Platform, The Broad Institute Genome Sequencing Center for Infectious Disease Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M., Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D., Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Borenstein D. , Brown A., Chapman S.B., Chen Z., Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L., Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D., Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Haas B., Nusbaum C., Birren B. Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 10403S.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF038438 Genomic DNA. Translation: AAC31362.1. CP002002 Genomic DNA. Translation: AEO05885.1.
RefSeq	YP_005962016.1. NC_017544.1.
3D structure databases
ProteinModelPortal	G2K4Q7.
SMR	G2K4Q7. Positions 4-367.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEO05885; AEO05885; LMRG_02310.
GeneID	12553107.
KEGG	lmt:LMRG_02310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031444.
KO	K01775.
Enzyme and pathway databases
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LISM4

Accession

Primary (citable) accession number: G2K4Q7
Secondary accession number(s): O85045

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	November 16, 2011
Last modified:	May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program