G2K2M9 (G2K2M9_LISM4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A HAMAP-Rule MF_00171
EC=5.4.99.12 HAMAP-Rule MF_00171

Alternative name(s):
tRNA pseudouridine(38-40) synthase HAMAP-Rule MF_00171
tRNA pseudouridylate synthase I HAMAP-Rule MF_00171
tRNA-uridine isomerase I HAMAP-Rule MF_00171

Gene names

Name:	truA HAMAP-Rule MF_00171
Ordered Locus Names:	LMRG_02142 EMBL AEO07580.1

Organism

Listeria monocytogenes serotype 1/2a (strain 10403S) [Complete proteome] [HAMAP] EMBL AEO07580.1

Taxonomic identifier

393133 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171 SAAS SAAS020094
Catalytic activity	tRNA uridine = tRNA pseudouridine. RuleBase RU003792 tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171 SAAS SAAS020094
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family. HAMAP-Rule MF_00171 RuleBase RU003792

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Molecular function	Isomerase HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	53	1	Nucleophile By similarity HAMAP-Rule MF_00171
	Binding site	111	1	Substrate By similarity HAMAP-Rule MF_00171

Sequences

	Sequence		Length	Mass (Da)
	G2K2M9 [UniParc]. Last modified November 16, 2011. Version 1. Checksum: 2B6717D3A11E7DA1	FASTA	248	28,399
	10 20 30 40 50 60 MTRYKAIISY DGSGFYGYQV QPNTRTVQAE IEKALTKMHK GKTVRVTASG RTDTGVHAKG 70 80 90 100 110 120 QVIHFDSELD ITAEKFQKAL QVMTPFDISF LTVEEVPDDF HARFGTVGKE YRYVVKRTKI 130 140 150 160 170 180 FDPFSRNFAL HYPYELDISK MKLASKRLIG EHDFTSFCSA RTERDSKVRT LYSIDFYEED 190 200 210 220 230 240 DETLVIAFQG NGFLYNMVRI LTGTLLDAGQ GRISPDDISE ALLARDRQKL ISKTAPPQGL YLWRVDYE

References

[1]

"The genome sequence of Listeria monocytogenes strain 10403S." The Broad Institute Genome Sequencing Platform, The Broad Institute Genome Sequencing Center for Infectious Disease. Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M., Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D., Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Borenstein D. , Brown A., Chapman S.B., Chen Z., Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L., Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D., Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Haas B., Nusbaum C., Birren B. Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 10403S.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002002 Genomic DNA. Translation: AEO07580.1.
RefSeq	YP_005963711.1. NC_017544.1.
3D structure databases
ProteinModelPortal	G2K2M9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEO07580; AEO07580; LMRG_02142.
GeneID	12554880.
KEGG	lmt:LMRG_02142.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K06173.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G2K2M9_LISM4

Accession

Primary (citable) accession number: G2K2M9

Entry history

Integrated into UniProtKB/TrEMBL:	November 16, 2011
Last sequence update:	November 16, 2011
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)