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Protein

Carnitine transport ATP-binding protein OpuCA

Gene

opuCA

Organism
Listeria monocytogenes serotype 1/2a (strain 10403S)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex OpuCABCD involved in carnitine uptake. Probably responsible for energy coupling to the transport system. Involved, with BetL and GbuABC, in osmoprotection and cryoprotection of Listeria. Can also mediate weak glycine betaine transport.3 Publications

Catalytic activityi

ATP + H2O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 428ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitine transport ATP-binding protein OpuCA (EC:3.6.3.32)
Gene namesi
Name:opuCA
Ordered Locus Names:LMRG_00880
OrganismiListeria monocytogenes serotype 1/2a (strain 10403S)
Taxonomic identifieri393133 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000001288 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Carnitine transport ATP-binding protein OpuCAPRO_0000418133Add
BLAST

Expressioni

Inductioni

The complex is induced by either hyperosmotic stress or by low temperature. Osmotic induction is sigma B-dependent.3 Publications

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).1 Publication

Structurei

3D structure databases

ProteinModelPortaliG2JZ44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 236235ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini255 – 31157CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini315 – 37359CBS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated
Contains 1 ABC transporter domain.PROSITE-ProRule annotation
Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

KOiK05847.
OMAiWGEEDQL.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G2JZ44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFEHVTKT YKGGKKAVND LTLNIDKGEF VCFIGPSGCG KTTTMKMINR
60 70 80 90 100
LIEPTEGKIF INDKDIMAED PVKLRRSIGY VIQQIGLMPH MTIRENIVLV
110 120 130 140 150
PKLLKWSEEK KQERAKELIK LVDLPEEFLD RYPYELSGGQ QQRIGVLRAL
160 170 180 190 200
AAEQNLILMD EPFGALDPIT RDSLQEEFKN LQKELGKTII FVTHDMDEAI
210 220 230 240 250
KLADRIVIMK DGEIVQFDTP DEILRNPANS FVEDFIGKDR LIEAKPDVTQ
260 270 280 290 300
VAQIMNTNPV SITADKSLQA AITVMKEKRV DTLLVVDEGN VLKGFIDVEQ
310 320 330 340 350
IDLNRRTATS VMDIIEKNVF YVYEDTLLRD TVQRILKRGY KYIPVVDKDK
360 370 380 390
RLVGIVTRAS LVDIVYDSIW GTLEDATENQ EEQADSKTTE PEMKQEG
Length:397
Mass (Da):45,209
Last modified:November 16, 2011 - v1
Checksum:i134CF427650AC83B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002002 Genomic DNA. Translation: AEO06413.1.
RefSeqiWP_003721933.1. NC_017544.1.

Genome annotation databases

EnsemblBacteriaiAEO06413; AEO06413; LMRG_00880.
KEGGilmt:LMRG_00880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002002 Genomic DNA. Translation: AEO06413.1.
RefSeqiWP_003721933.1. NC_017544.1.

3D structure databases

ProteinModelPortaliG2JZ44.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEO06413; AEO06413; LMRG_00880.
KEGGilmt:LMRG_00880.

Phylogenomic databases

KOiK05847.
OMAiWGEEDQL.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Listeria monocytogenes strain 10403S."
    The Broad Institute Genome Sequencing Platform, The Broad Institute Genome Sequencing Center for Infectious Disease
    Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M., Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D., Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Borenstein D.
    , Brown A., Chapman S.B., Chen Z., Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L., Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D., Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Haas B., Nusbaum C., Birren B.
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 10403S.
  2. "Identification of opuC as a chill-activated and osmotically activated carnitine transporter in Listeria monocytogenes."
    Angelidis A.S., Smith L.T., Hoffman L.M., Smith G.M.
    Appl. Environ. Microbiol. 68:2644-2650(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBUNIT.
    Strain: 10403S.
  3. "Gbu glycine betaine porter and carnitine uptake in osmotically stressed Listeria monocytogenes cells."
    Mendum M.L., Smith L.T.
    Appl. Environ. Microbiol. 68:5647-5655(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CARNITINE UPTAKE.
    Strain: 10403S.
  4. "Three transporters mediate uptake of glycine betaine and carnitine by Listeria monocytogenes in response to hyperosmotic stress."
    Angelidis A.S., Smith G.M.
    Appl. Environ. Microbiol. 69:1013-1022(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CARNITINE AND GLYCINE BETAINE UPTAKE, INDUCTION.
    Strain: 10403S.
  5. "Role of sigmaB in regulating the compatible solute uptake systems of Listeria monocytogenes: osmotic induction of opuC is sigmaB dependent."
    Fraser K.R., Sue D., Wiedmann M., Boor K., O'Byrne C.P.
    Appl. Environ. Microbiol. 69:2015-2022(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 10403S.

Entry informationi

Entry nameiOPUCA_LISM4
AccessioniPrimary (citable) accession number: G2JZ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: November 16, 2011
Last modified: November 11, 2015
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.