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G2IZV2 (G2IZV2_PSEUL) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_00098
EC=6.1.1.10 HAMAP-Rule MF_00098
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_00098
Gene names
Name:metG HAMAP-Rule MF_00098
Ordered Locus Names:NH8B_1069 EMBL BAK75900.1
OrganismPseudogulbenkiania sp. (strain NH8B) [Complete proteome] [HAMAP]
Taxonomic identifier748280 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Pseudogulbenkiania

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098 SAAS SAAS002547

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098 SAAS SAAS002547

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098 SAAS SAAS002547

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00098 SAAS SAAS002547

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00098 SAAS SAAS002547.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. HAMAP-Rule MF_00098

Contains 1 tRNA-binding domain. HAMAP-Rule MF_00098 SAAS SAAS002547

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain598 – 702105tRNA-binding By similarity HAMAP-Rule MF_00098
Motif14 – 2411"HIGH" region By similarity HAMAP-Rule MF_00098
Motif340 – 3445"KMSKS" region By similarity HAMAP-Rule MF_00098

Sites

Metal binding1451Zinc By similarity HAMAP-Rule MF_00098
Metal binding1481Zinc By similarity HAMAP-Rule MF_00098
Metal binding1581Zinc By similarity HAMAP-Rule MF_00098
Metal binding1611Zinc By similarity HAMAP-Rule MF_00098
Binding site3431ATP By similarity HAMAP-Rule MF_00098

Sequences

Sequence LengthMass (Da)Tools
G2IZV2 [UniParc].

Last modified November 16, 2011. Version 1.
Checksum: 86FF0AC905F3D64D

FASTA70278,091
        10         20         30         40         50         60 
MTSPRKILVT SALPYANGAI HLGHMVEHIQ TDVWVRFQKM RGHACHYVCA DDTHGTPIML 

        70         80         90        100        110        120 
AAEKQGITPE QLIERVKAEH LADFTGFHIG YDNYYSTNSP ENKHFAEHIY RTLRANGKIV 

       130        140        150        160        170        180 
SRTIEQLFDP EKQMFLPDRF VKGECPKCGA KDQYGDNCES CGATYSPTDL KNPYSAVSGA 

       190        200        210        220        230        240 
TPVLKSSEHY FFRLGDCADF LKAWTAGSSV RADGETQPHL QPESLNKMNE WIEGGLHDWD 

       250        260        270        280        290        300 
ISRDAPYFGF EIPDAPGKYF YVWLDAPIGY MASFKNLCEK SGLDFDDYFR PDSDAELYHF 

       310        320        330        340        350        360 
IGKDILYFHA LFWPAMLKYS GYRAPTGVFA HGFLTVDGQK MSKSRGTFIT AKSYLDCGLN 

       370        380        390        400        410        420 
PEWMRYYVAA KLNARIEDID LSLDDFVARV NSDLIGKFVN IASRAAGFIT KRFGGRLAES 

       430        440        450        460        470        480 
VSSSALASPV CHAAQCEHPL LQQLAESSAT LAAAYEAREY ARALRDVMAL ADEVNAYVDA 

       490        500        510        520        530        540 
NKPWELAKQE GMEARLHEVC TVLVNAFRLL AIYLKPVLPK LAADVEAFLN IAPLTWDDAD 

       550        560        570        580        590        600 
TLLLGHTIKP YQHLMQRVEA AQIDKLIEAN KQSMEQQAAP AEASANYEPL ADTITIDDFA 

       610        620        630        640        650        660 
KLDLRVGKVL ACQFVDGSDK LLQFTVDLGF ETRNIFSGIR QAYQEPDKLV GRNVIVVANL 

       670        680        690        700 
APRKMRFGVS AGMIVCASGN DDTSGLFLLD TDDGAQPGMR IG

« Hide

References

[1]"Complete genome sequence of the denitrifying and N(2)O-reducing bacterium Pseudogulbenkiania sp. strain NH8B."
Ishii S., Tago T., Nishizawa T., Oshima K., Hattori M., Senoo K.
J. Bacteriol. 193:6395-6396(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NH8B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP012224 Genomic DNA. Translation: BAK75900.1.
RefSeqYP_004846314.1. NC_016002.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAK75900; BAK75900; NH8B_1069.
GeneID11154397.
KEGGpse:NH8B_1069.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01874.

Enzyme and pathway databases

BioCycPSP748280:GHJ9-1069-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00098. Met_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG2IZV2_PSEUL
AccessionPrimary (citable) accession number: G2IZV2
Entry history
Integrated into UniProtKB/TrEMBL: November 16, 2011
Last sequence update: November 16, 2011
Last modified: May 29, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info