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Protein

ATP-dependent 6-phosphofructokinase

Gene

NDAI0I00590

Organism
Naumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639) (Saccharomyces dairenensis)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (NDAI0C05500)
  3. ATP-dependent 6-phosphofructokinase (NDAI0I00590), ATP-dependent 6-phosphofructokinase (NDAI0D02620)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei213ATP; via amide nitrogenUniRule annotation1
Metal bindingi308Magnesium; catalyticUniRule annotation1
Active sitei355Proton acceptorUniRule annotation1
Binding sitei390Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei454SubstrateUniRule annotation1
Binding sitei482Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei665Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei761Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei854Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei942Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi277 – 278ATPUniRule annotation2
Nucleotide bindingi307 – 310ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:NDAI0I00590Imported
Ordered Locus Names:NDAI_0I00590Imported
OrganismiNaumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639) (Saccharomyces dairenensis)Imported
Taxonomic identifieri1071378 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNaumovozyma
Proteomesi
  • UP000000689 Componenti: Chromosome 9

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi1071378.XP_003671871.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 513PFKInterPro annotationAdd BLAST308
Domaini595 – 886PFKInterPro annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 580N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST580
Regioni353 – 355Substrate bindingUniRule annotation3
Regioni397 – 399Substrate bindingUniRule annotation3
Regioni488 – 491Substrate bindingUniRule annotation4
Regioni581 – 594Interdomain linkerUniRule annotationAdd BLAST14
Regioni595 – 966C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST372
Regioni723 – 727Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni768 – 770Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni860 – 863Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
KOiK00850.
OrthoDBiEOG092C0LOE.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G0WFR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTTPLVNG TSYCLIKAYS QDSYKQAIEF YSKFLALDDC STDNTTQTTT
60 70 80 90 100
LSNGSISLKI KLEEDKTLQS KTESQIQFLK SIKDTKDWRS HVAESLVFNT
110 120 130 140 150
SNIMLNKDTL TTLNAPTQSF PSDLFPMQLY TIDPLGNIIG VTSTKNAVST
160 170 180 190 200
MPTEPPSTKK AKFSTSTLPS KVHSFTDLSY RMQTTNNYPS LPGKQIIDPT
210 220 230 240 250
KPKKAIAVMT SGGDAQGMNS NVRAIVRSAI FKGCRAFVVM EGYEGLVRGG
260 270 280 290 300
PEYIKECSWE DVRGWSTEGG TNIGTARCLE FRQRDGRLLG AQHLIEAGVD
310 320 330 340 350
ALIVCGGDGS LTGADLFRSE WPSLIKELLE NGRISKDQFQ KYQHLNICGT
360 370 380 390 400
VGSIDNDMST TDATIGAYSA LDRICKAIDY VEATANSHSR AFVVEVMGRN
410 420 430 440 450
CGWLALLAGI ATSADYIFIP EKPATSSEWQ DQMCDIVAKH RSKGKRTTIV
460 470 480 490 500
IVAEGAISAD LTPISPADVH KVLVDRLGLD TRITTLGHVQ RGGTAVAYDR
510 520 530 540 550
MLATLQGVEA VNAVLESTAD TPSPLIAINE NKIVRKPLVE SVRLTKSVAE
560 570 580 590 600
AIQAKDFKKA MDLRDTEFVE HLKNFMAINS ADHNEPKLPN DKRLKIAIVN
610 620 630 640 650
VGAPAGGINS AVYSMATYCM SQGHKPYAIN NGWSGLARHE SVRSLEWKDM
660 670 680 690 700
IGWQSRGGSE IGTNRVTPEQ ADIGMIAYYF QKYQFDGLVI VGGFEAFESL
710 720 730 740 750
HQLERARESY PAFRIPMVLI PATLSNNVPG TEYSLGSDTA LNALMEYCDV
760 770 780 790 800
VKQSASSTRG RAFVVDVQGG NSGYLATCAA LAVGAQASYV PEEGIPLEQL
810 820 830 840 850
QQDIETLAES FESAKGRGRF GKLILKSTNA SKALSATDLA KVMTSEANGR
860 870 880 890 900
FDAKPAYPGH VQQGGLPSPI DRTRATRFAI RAVDFIEEQQ ETIAAARAAD
910 920 930 940 950
DDFKLDDKVV SATATVLGVR RSHIVFNPIR QLYDFETDVE ARMPKVIHWQ
960
ATREIADHLV GRKRVD
Length:966
Mass (Da):105,518
Last modified:October 19, 2011 - v1
Checksum:i1FA7DBD40ABF010D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE580275 Genomic DNA. Translation: CCD26628.1.
RefSeqiXP_003671871.1. XM_003671823.1.

Genome annotation databases

EnsemblFungiiCCD26628; CCD26628; NDAI_0I00590.
GeneIDi11496186.
KEGGindi:NDAI_0I00590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE580275 Genomic DNA. Translation: CCD26628.1.
RefSeqiXP_003671871.1. XM_003671823.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1071378.XP_003671871.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCCD26628; CCD26628; NDAI_0I00590.
GeneIDi11496186.
KEGGindi:NDAI_0I00590.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
KOiK00850.
OrthoDBiEOG092C0LOE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG0WFR7_NAUDC
AccessioniPrimary (citable) accession number: G0WFR7
Entry historyi
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: September 7, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.