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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

NCAS0F01220

Organism
Naumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei471 – 4711S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. histone-lysine N-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulatorUniRule annotation, MethyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

Transcription, Transcription regulationUniRule annotation

Keywords - Ligandi

S-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specificUniRule annotation (EC:2.1.1.43UniRule annotation)
Alternative name(s):
Histone H3-K79 methyltransferaseUniRule annotation
Gene namesi
Name:NCAS0F01220Imported
Ordered Locus Names:NCAS_0F01220Imported
OrganismiNaumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)Imported
Taxonomic identifieri1064592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNaumovozyma
ProteomesiUP000001640: Chromosome 6

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

NucleusUniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni421 – 4244S-adenosyl-L-methionine bindingUniRule annotation
Regioni444 – 45310S-adenosyl-L-methionine bindingUniRule annotation
Regioni508 – 5092S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.UniRule annotation

Phylogenomic databases

InParanoidiG0VGI5.
KOiK11427.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR013110. DOT1_dom_Pfam.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G0VGI5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTNTVPFTP SPSSSSIFST STTNFNNNNS RDSSMAPADS TEASLTEQTK
60 70 80 90 100
KGKTRAKGSS AVEKLLEEAN RYHPQYEYSL PQGFLRTRKS KAASPSEEQA
110 120 130 140 150
SDLAKDDNLV KRKMEEEEDY DTSTTAAKKV RKNKVTSTSS TKKRKRKIKK
160 170 180 190 200
AETNGTTGSK PTKKRKDTIK RPLSPSAALA DSVRRMSISA MLDDSDTKDG
210 220 230 240 250
TGIYNEQGVK LDPVANKDDR AMTSTFLDWD QPYLELQYPL FNVDSLKLTN
260 270 280 290 300
VYKGNPIKST ILTSLTHHQS RHKPPSLSND SSIKFVHLQS PLFVNYQEEY
310 320 330 340 350
MINFEKDLQR YNPMSEIGKL IEYTALVYLP STYSEKLKRE VIPSLNQAFD
360 370 380 390 400
NSDTDRFISS VERYNSIIRM VPRHEILEHL KTITQIPKSF IHDFLHIIYT
410 420 430 440 450
RSIHPHASKL KHYKAFSNFV YGELLPNFLS EVFSQCNLKP NCTFMDLGSG
460 470 480 490 500
VGNCVIQASL EYGLKKSFGC EIMPDASELT ELQMVELKER GKLFGFNLSD
510 520 530 540 550
IEFSLRESFV DNPKVDELIK DCDLLLVNNF LFDSKLNEKV EKITQNLKTG
560 570 580 590 600
CKIVSLKNLR SFSYKIDFFN MENILSRLKV EKVLLKEDSV SWTHSGGEYY
610 620 630
IATVMENIDE SLFDPVVRGR NTRRPTKYSR
Length:630
Mass (Da):71,757
Last modified:October 19, 2011 - v1
Checksum:i8B0BC9ACFD232324
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE576757 Genomic DNA. Translation: CCC70606.1.
RefSeqiXP_003676961.1. XM_003676913.1.

Genome annotation databases

GeneIDi11527634.
KEGGincs:NCAS_0F01220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE576757 Genomic DNA. Translation: CCC70606.1.
RefSeqiXP_003676961.1. XM_003676913.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11527634.
KEGGincs:NCAS_0F01220.

Phylogenomic databases

InParanoidiG0VGI5.
KOiK11427.
OrthoDBiEOG7KH9VN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR013110. DOT1_dom_Pfam.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evolutionary erosion of yeast sex chromosomes by mating-type switching accidents."
    Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P., Wolfe K.H.
    Proc. Natl. Acad. Sci. U.S.A. 108:20024-20029(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630Imported.
  2. "Genome sequence of Naumovozyma castellii."
    Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P., Wolfe K.H.
    Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Type strain:CBS 4309.

Entry informationi

Entry nameiG0VGI5_NAUCC
AccessioniPrimary (citable) accession number: G0VGI5
Entry historyi
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: January 7, 2015
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.