ID   G0L998_ZOBGA            Unreviewed;       477 AA.
AC   G0L998;
DT   19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT   19-OCT-2011, sequence version 1.
DT   27-MAR-2024, entry version 52.
DE   SubName: Full=Alpha-amylase, family GH13 {ECO:0000313|EMBL:CAZ94442.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:CAZ94442.1};
GN   Name=susA {ECO:0000313|EMBL:CAZ94442.1};
GN   OrderedLocusNames=zobellia_369 {ECO:0000313|EMBL:CAZ94442.1};
OS   Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 /
OS   NCIMB 13871 / Dsij).
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Zobellia.
OX   NCBI_TaxID=63186 {ECO:0000313|EMBL:CAZ94442.1, ECO:0000313|Proteomes:UP000008898};
RN   [1] {ECO:0000313|Proteomes:UP000008898}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij
RC   {ECO:0000313|Proteomes:UP000008898};
RG   Genoscope - CEA;
RT   "Complete genome sequence of Zobellia galactanivorans Dsij.";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CAZ94442.1, ECO:0000313|Proteomes:UP000008898}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij
RC   {ECO:0000313|Proteomes:UP000008898};
RX   PubMed=22513138; DOI=10.1111/j.1462-2920.2012.02751.x;
RA   Thomas F., Barbeyron T., Tonon T., Genicot S., Czjzek M., Michel G.;
RT   "Characterization of the first alginolytic operons in a marine bacterium:
RT   from their emergence in marine Flavobacteriia to their independent
RT   transfers to marine Proteobacteria and human gut Bacteroides.";
RL   Environ. Microbiol. 14:2379-2394(2012).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; FP476056; CAZ94442.1; -; Genomic_DNA.
DR   RefSeq; WP_013991755.1; NC_015844.1.
DR   AlphaFoldDB; G0L998; -.
DR   STRING; 63186.ZOBELLIA_369; -.
DR   KEGG; zga:ZOBELLIA_369; -.
DR   PATRIC; fig|63186.3.peg.371; -.
DR   HOGENOM; CLU_024572_3_0_10; -.
DR   OrthoDB; 9806009at2; -.
DR   Proteomes; UP000008898; Chromosome.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF09154; Alpha-amy_C_pro; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Glycosidase {ECO:0000313|EMBL:CAZ94442.1};
KW   Hydrolase {ECO:0000313|EMBL:CAZ94442.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008898}.
FT   DOMAIN          52..411
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        245
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        269
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         152
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   477 AA;  54386 MW;  28D24825DBF25B42 CRC64;
     MKKIYRFFCF GLAIMASCSS DDDQSVVADT NDNTQKEIIS PIELSDYDNG SGVMMQTFYW
     DVEPRGEWWT NLSGKIDDWA ESGVDRLWLP VATKGASGGY SMGYDPSDYF DFGEFDQHGT
     VETRFGSRQE LETLIEKAHG KGLEVIADIV INHNSGGGEE YNPYRDKNTY TLFDELHGNA
     SGMFNRNFEN FYPNSTSNYD DGSLFYAEQN LDHNQEYVQN WLWKSENSVA KYYKNVMGFD
     GWRFDYVLGY EPWVVKAWLD EVGGFSVSEL WDGNADVLSQ YVDQTGSGVF DFATFYKLEE
     AMDRFDDLTV LEGKMLWKTH PDKSVTFAAN HDTEKDENED NRIADENKLK AYAYILTHPG
     YPTIFYSDYE NEAFKEPLKQ LVQIHNSLAI GDAEVLYVDN DEYIMKRAGT DDNPGLILYI
     NTSGNTKKRT IKSAWNNSYL MDYTKNVHGS ITSDENGEAL IEAPANGYSI WSIAKVN
//