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Protein

DNA ligase

Gene

lig

Organism
Thermococcus sp. (strain CGMCC 1.5172 / 4557)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation
NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei247 – 2471ATPUniRule annotation
Active sitei249 – 2491N6-AMP-lysine intermediateUniRule annotation
Binding sitei254 – 2541ATPUniRule annotation
Binding sitei269 – 2691ATPUniRule annotation
Binding sitei299 – 2991ATPUniRule annotation
Binding sitei339 – 3391ATPUniRule annotation
Binding sitei414 – 4141ATPUniRule annotation
Binding sitei420 – 4201ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSP1042877:GHEX-1604-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation, EC:6.5.1.6UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:GQS_07890Imported
OrganismiThermococcus sp. (strain CGMCC 1.5172 / 4557)Imported
Taxonomic identifieri1042877 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000874 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliG0HLK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.
OMAiGRPRPFQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G0HLK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYAELADLY RRLEKTTLKT LKTKFVSDFL KKAPDELLDI IPYLILGKVF
60 70 80 90 100
PDWDERELGV GEKLLIKAVS MATGVPEREI ENSVRDTGDL GESVALALKK
110 120 130 140 150
KKQKSFFSQP LTIKRVYDTF MKIAEASGQG SQDRKLKYLA NLFMDAQPEE
160 170 180 190 200
GKYLARTVLG TMRTGVAEGL MRDAIASAFG VKAELVERAY MLTSDFGYVA
210 220 230 240 250
RVAKLEGNEG LSKVRIQVGK PIRPMLAQNA ANVKEALVEM GGKAAFEVKY
260 270 280 290 300
DGARVQVHKD GDRVVIYSRR LENVTRSIPE VVDAVLESVK PEKAIVEGEL
310 320 330 340 350
VAVGEGGKPR PFQYVLRRFR RKYNIEEMVE KIPLELNLFD VLYVDGDGMI
360 370 380 390 400
DTPFSERRKK LEEIISQNER IRLAEQLVTT SADEAEEFYQ RALELGHEGL
410 420 430 440 450
MAKRLDSVYE PGNRGKKWLK IKPTMEDLDL VIIGAEWGEG RRAHLLGSFL
460 470 480 490 500
VAAFDPHSGE FVPVGKVGSG FTDEDLAEFT RMLKPLIVRE EGKYVEIEPK
510 520 530 540 550
VVIQVTYQEI QKSPKYESGF ALRFPRYVAL REDKSPEEAD TIERIAQLYE

FQERFKAKR
Length:559
Mass (Da):63,338
Last modified:October 18, 2011 - v1
Checksum:iD415CBE4FF3B6BA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002920 Genomic DNA. Translation: AEK73474.1.
RefSeqiWP_014013156.1. NC_015865.1.
YP_004763151.1. NC_015865.1.

Genome annotation databases

EnsemblBacteriaiAEK73474; AEK73474; GQS_07890.
GeneIDi11016253.
KEGGithe:GQS_07890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002920 Genomic DNA. Translation: AEK73474.1.
RefSeqiWP_014013156.1. NC_015865.1.
YP_004763151.1. NC_015865.1.

3D structure databases

ProteinModelPortaliG0HLK1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEK73474; AEK73474; GQS_07890.
GeneIDi11016253.
KEGGithe:GQS_07890.

Phylogenomic databases

KOiK10747.
OMAiGRPRPFQ.

Enzyme and pathway databases

BioCyciTSP1042877:GHEX-1604-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Thermococcus sp. strain 4557, a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area."
    Wang X., Gao Z., Xu X., Ruan L.
    J. Bacteriol. 193:5544-5545(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CGMCC 1.5172 / 4557Imported.

Entry informationi

Entry nameiG0HLK1_THES4
AccessioniPrimary (citable) accession number: G0HLK1
Entry historyi
Integrated into UniProtKB/TrEMBL: October 18, 2011
Last sequence update: October 18, 2011
Last modified: February 3, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.