Skip Header

Contribute Send feedback
Read comments (?) or add your own

G0G1G3 (G0G1G3_AMYMD) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL AFO82751.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:AMES_9219 EMBL AFO82751.1, RAM_47985 EMBL AEK48062.1
OrganismAmycolatopsis mediterranei S699 EMBL AEK48062.1
Taxonomic identifier713604 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis ›

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS000291

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS000291

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS000291

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS000291

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS000291.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS000291

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain102 – 309208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding136 – 19156ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2631Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2761Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2761Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2781Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G0G1G3 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: A6FD5F392EC6E661

FASTA31432,874
        10         20         30         40         50         60 
MVDRTVAVLA GGLSHERDVS LRSGRRLSAA LKSESFGIEE WDTDAGLLER LRTQRPDAVV 

        70         80         90        100        110        120 
VALHGGEGEN GSVQTVLEML GVPFVGTGSQ GCRRAWDKPT AKALVQNAGF VTPGWVVLPH 

       130        140        150        160        170        180 
STFRELGAQA VLDAMVETLG LPLILKPDQG GSALGTQVVR EAAELPAAMV GCFAYGDTVL 

       190        200        210        220        230        240 
AERFVDGVEV AVTVIEGEDG PEALPAVEIV PESGVYDYTA RYTAGLTDFF TPARLDDAAA 

       250        260        270        280        290        300 
KAVAELAVAA HRVLGLRDIS RTDAVVMADG TVHFLEVNPS PGLTETSTVP MAIEAAGKSL 

       310 
GAVFGELIGR AISR

« Hide

References

[1]"Whole Genome Sequence of the Rifamycin B-Producing Strain Amycolatopsis mediterranei S699."
Verma M., Kaur J., Kumar M., Kumari K., Saxena A., Anand S., Nigam A., Ravi V., Raghuvanshi S., Khurana P., Tyagi A.K., Khurana J.P., Lal R.
J. Bacteriol. 193:5562-5563(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: S699 EMBL AEK48062.1.
[2]"Complete Genome Sequence of Amycolatopsis mediterranei S699 Based on De Novo Assembly via a Combinatorial Sequencing Strategy."
Tang B., Zhao W., Zheng H., Zhuo Y., Zhang L., Zhao G.P.
J. Bacteriol. 194:5699-5700(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: S699 EMBL AFO82751.1.
[3]Biao T.
Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: S699 EMBL AFO82751.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002896 Genomic DNA. Translation: AEK48062.1.
CP003729 Genomic DNA. Translation: AFO82751.1.
RefSeqYP_005537519.1. NC_017186.1.
YP_006555696.1. NC_018266.1.

3D structure databases

ProteinModelPortalG0G1G3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEK48062; AEK48062; RAM_47985.
AFO82751; AFO82751; AMES_9219.
GeneID12122030.
13411339.
KEGGamm:AMES_9219.
amn:RAM_47985.

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG0G1G3_AMYMD
AccessionPrimary (citable) accession number: G0G1G3
Entry history
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: May 29, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info