G0FKP2 (G0FKP2_AMYMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-isopropylmalate synthase HAMAP-Rule MF_00572
EC=2.3.3.13 HAMAP-Rule MF_00572

Alternative name(s):
Alpha-IPM synthase HAMAP-Rule MF_00572
Alpha-isopropylmalate synthase HAMAP-Rule MF_00572

Gene names

Name:	leuA HAMAP-Rule MF_00572 EMBL AFO82283.1
ORF Names:	AMES_8751 EMBL AFO82283.1, RAM_45590 EMBL AEK47583.1

Organism

Amycolatopsis mediterranei S699 EMBL AEK47583.1

Taxonomic identifier

713604 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis › Amycolatopsis mediterranei

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_00572
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. SAAS SAAS005668 HAMAP-Rule MF_00572
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. SAAS SAAS002034 HAMAP-Rule MF_00572
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00572
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. HAMAP-Rule MF_00572

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis HAMAP-Rule MF_00572 SAAS SAAS005668
Molecular function	Transferase SAAS SAAS002034 HAMAP-Rule MF_00572
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	G0FKP2 [UniParc]. Last modified October 19, 2011. Version 1. Checksum: 75344EEE767F198B	FASTA	561	62,838
	10 20 30 40 50 60 MPVHRYRPWY DVVENIDLPD RTWPEKRIDR APLWCAVDLR DGNQALIDPM SPARKRKFFD 70 80 90 100 110 120 LLVRMGYKEI EVGFPAASQT DFDFVREIIE EHAIPDDVSI QVLTQCRPEL IERTFKSLEG 130 140 150 160 170 180 APRAIVHIYN STSILQRRVV FREEREGIKK IATQAAEMVV ELAAKQPDTD FRFQYSPESY 190 200 210 220 230 240 TGTELSYALE VCNAVTEIWQ PTPEKPVILN LPATVEMATP NVYADSIEWM SRNLDRRDSV 250 260 270 280 290 300 ILSLHPHNDR GTGIAAAELG YQAGADRIEG CLFGNGERTG NVDLVALGMN LYSQGIDPQI 310 320 330 340 350 360 DFSDMDEIKR TVEYCNQLPV GERSPWGGDL VFTAFSGSHQ DAINKGLDAL KDAADKAGVP 370 380 390 400 410 420 IDEYPWEVPY LPIDPKDVGR MYEAVIRVNS QSGKGGVAYI MKAEHQLDLP RRLQIEFSKV 430 440 450 460 470 480 IQRYTDTEGG EVDPATMYNA FSAEYLELKT PLELVRQHVR DNGDGEYDIT ATVKVEGDEH 490 500 510 520 530 540 EVTGRGNGPI AAFFDALSTV GFDLRLLDYN EHTLSPGDDA RAASYIECAI SDRVFWGIGI 550 560 DPSIVTASLR AVVSAVNRAN R

References

[1]	"Whole Genome Sequence of the Rifamycin B-Producing Strain Amycolatopsis mediterranei S699." Verma M., Kaur J., Kumar M., Kumari K., Saxena A., Anand S., Nigam A., Ravi V., Raghuvanshi S., Khurana P., Tyagi A.K., Khurana J.P., Lal R. J. Bacteriol. 193:5562-5563(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AEK47583.1.
[2]	"Complete Genome Sequence of Amycolatopsis mediterranei S699 Based on De Novo Assembly via a Combinatorial Sequencing Strategy." Tang B., Zhao W., Zheng H., Zhuo Y., Zhang L., Zhao G.P. J. Bacteriol. 194:5699-5700(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AFO82283.1.
[3]	Biao T. Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AFO82283.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002896 Genomic DNA. Translation: AEK47583.1. CP003729 Genomic DNA. Translation: AFO82283.1.
RefSeq	YP_005537040.1. NC_017186.1. YP_006555228.1. NC_018266.1.
3D structure databases
ProteinModelPortal	G0FKP2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEK47583; AEK47583; RAM_45590. AFO82283; AFO82283; AMES_8751.
GeneID	12125827. 13409574.
KEGG	amm:AMES_8751. amn:RAM_45590.
Phylogenomic databases
KO	K01649.
Enzyme and pathway databases
UniPathway	UPA00048; UER00070.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00572. LeuA_type2.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005668. IPM_Synthase. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00970. leuA_yeast. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G0FKP2_AMYMD

Accession

Primary (citable) accession number: G0FKP2

Entry history

Integrated into UniProtKB/TrEMBL:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)