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G0F028 (G0F028_CUPNN) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycine dehydrogenase (decarboxylating) HAMAP-Rule MF_00711

EC=1.4.4.2 HAMAP-Rule MF_00711
Alternative name(s):
Glycine cleavage system P-protein HAMAP-Rule MF_00711
Glycine decarboxylase HAMAP-Rule MF_00711
Glycine dehydrogenase (aminomethyl-transferring) HAMAP-Rule MF_00711
Gene names
Name:gcvP HAMAP-Rule MF_00711 EMBL AEI78864.1
Ordered Locus Names:CNE_1c35720 EMBL AEI78864.1
OrganismCupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha) [Complete proteome] [HAMAP] EMBL AEI78864.1
Taxonomic identifier1042878 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length976 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00711 SAAS SAAS020580

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. SAAS SAAS020580 HAMAP-Rule MF_00711

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00711 SAAS SAAS020580

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H By similarity. SAAS SAAS020580 HAMAP-Rule MF_00711

Sequence similarities

Belongs to the GcvP family. HAMAP-Rule MF_00711

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Amino acid modifications

Modified residue7221N6-(pyridoxal phosphate)lysine By similarity HAMAP-Rule MF_00711

Sequences

Sequence LengthMass (Da)Tools
G0F028 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: A9A9D4EC2E7B7DC2

FASTA976104,999
        10         20         30         40         50         60 
MNAPLPMNAA QVTRPTLAEL EARDAFAARH IGPDTPEQQH MLKVLGYDSR AALIDAVIPA 

        70         80         90        100        110        120 
AIRRRDGMPM GEFTEPLSEE AALAKLRGLA GRNKVLKSFI GQGYYNTVTP GVILRNIFEN 

       130        140        150        160        170        180 
PAWYTAYTPY QPEISQGRLE AMLNFQQMVT DLTGLDIANA SMLDEGTAAA EAMTLLQRVN 

       190        200        210        220        230        240 
KHDSNTFYVA DDVLPQTLEV VRTRALPMGI EVKVGPAAEA AAANAFGVLL QYPGVNGDVN 

       250        260        270        280        290        300 
DYGAIADAVH AAGGLVVAAA DLLALTLIAA PGEWGADVAV GNSQRFGVPL GFGGPHAGYM 

       310        320        330        340        350        360 
AVKDAFKRSM PGRLVGVTID AQGNKAYRLA LQTREQHIRR EKATSNICTA QVLLAVMASM 

       370        380        390        400        410        420 
YAVYHGPQGL KRIAQRVHRL TATLAAGLQA LGFARTNASF FDTLTLETGF NTDAIHAAAT 

       430        440        450        460        470        480 
ARGINLRHIS ATRVGISLDE TASRADVVAL WEVFTQGKPL PAELDFDKLE AVAQDAFPAA 

       490        500        510        520        530        540 
LARASEYLTH PVFNTHHAEH EMLRYLRMLA DKDLALDRTM IPLGSCTMKL NATSEMIPVT 

       550        560        570        580        590        600 
WPEFSQIHPF APLDQTVGYR EMIDQLEAML CAATGYAAVS LQPNAGSQGE YAGLLIIHAY 

       610        620        630        640        650        660 
HASRGESHRD ICLIPSSAHG TNPASAQMAG MKVVVVACDE DGNVDLQDLA KKAEQHSKNL 

       670        680        690        700        710        720 
AAIMITYPST HGVFEQGVQQ ICEIVHQHGG QVYVDGANMN AMVGTAAPGQ FGGDVSHLNL 

       730        740        750        760        770        780 
HKTFCIPHGG GGPGVGPVAV GAHLADFLPN QDSVGYRRDD RGIGGVSAAP FGSASILPIS 

       790        800        810        820        830        840 
WMYIAMMGSA GLTAATENAI LAANYVAKRL APYYPVLYTG QHDLVAHECI LDLRPLQKDT 

       850        860        870        880        890        900 
GISNEDVAKR LMDYGFHAPT MSFPVPGTLM IEPTESEALH ELDRFIDAMI AIRQEIGRVA 

       910        920        930        940        950        960 
DGTFDRDDNP LKHAPHTAAV VTADEWTHKY TREEAAYPVA SLRTQKYWPP VGRADNVYGD 

       970 
RNLFCACVPV SDYVVD 

« Hide

References

[1]"Complete Genome Sequence of the Type Strain Cupriavidus necator N-1."
Poehlein A., Kusian B., Friedrich B., Daniel R., Bowien B.
J. Bacteriol. 193:5017-5017(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43291 / DSM 13513 / N-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002877 Genomic DNA. Translation: AEI78864.1.
RefSeqYP_004687345.1. NC_015726.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEI78864; AEI78864; CNE_1c35720.
GeneID10919963.
KEGGcnc:CNE_1c35720.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00281.

Enzyme and pathway databases

BioCycCNEC1042878:GH0Z-3572-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 2 hits.
HAMAPMF_00711. GcvP.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMSSF53383. SSF53383. 3 hits.
TIGRFAMsTIGR00461. gcvP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameG0F028_CUPNN
AccessionPrimary (citable) accession number: G0F028
Entry history
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)