G0F028 (G0F028_CUPNN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glycine dehydrogenase [decarboxylating] HAMAP-Rule MF_00711
EC=1.4.4.2 HAMAP-Rule MF_00711

Alternative name(s):
Glycine cleavage system P-protein HAMAP-Rule MF_00711
Glycine decarboxylase HAMAP-Rule MF_00711

Gene names

Name:	gcvP HAMAP-Rule MF_00711
Ordered Locus Names:	CNE_1c35720 EMBL AEI78864.1

Organism

Cupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha) [Complete proteome] [HAMAP] EMBL AEI78864.1

Taxonomic identifier

1042878 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	976 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. SAAS SAAS020581 HAMAP-Rule MF_00711
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. SAAS SAAS020581 HAMAP-Rule MF_00711
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_00711 SAAS SAAS020581
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity. SAAS SAAS020581 HAMAP-Rule MF_00711
Sequence similarities	Belongs to the GcvP family. HAMAP-Rule MF_00711

Ontologies

Keywords
Ligand	Pyridoxal phosphate SAAS SAAS020581 HAMAP-Rule MF_00711
Molecular function	Oxidoreductase SAAS SAAS020581 HAMAP-Rule MF_00711 EMBL AEI78864.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Molecular_function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Amino acid modifications
	Modified residue	722	1	N6-(pyridoxal phosphate)lysine By similarity HAMAP-Rule MF_00711

Sequences

	Sequence		Length	Mass (Da)
	G0F028 [UniParc]. Last modified October 19, 2011. Version 1. Checksum: A9A9D4EC2E7B7DC2	FASTA	976	104,999
	10 20 30 40 50 60 MNAPLPMNAA QVTRPTLAEL EARDAFAARH IGPDTPEQQH MLKVLGYDSR AALIDAVIPA 70 80 90 100 110 120 AIRRRDGMPM GEFTEPLSEE AALAKLRGLA GRNKVLKSFI GQGYYNTVTP GVILRNIFEN 130 140 150 160 170 180 PAWYTAYTPY QPEISQGRLE AMLNFQQMVT DLTGLDIANA SMLDEGTAAA EAMTLLQRVN 190 200 210 220 230 240 KHDSNTFYVA DDVLPQTLEV VRTRALPMGI EVKVGPAAEA AAANAFGVLL QYPGVNGDVN 250 260 270 280 290 300 DYGAIADAVH AAGGLVVAAA DLLALTLIAA PGEWGADVAV GNSQRFGVPL GFGGPHAGYM 310 320 330 340 350 360 AVKDAFKRSM PGRLVGVTID AQGNKAYRLA LQTREQHIRR EKATSNICTA QVLLAVMASM 370 380 390 400 410 420 YAVYHGPQGL KRIAQRVHRL TATLAAGLQA LGFARTNASF FDTLTLETGF NTDAIHAAAT 430 440 450 460 470 480 ARGINLRHIS ATRVGISLDE TASRADVVAL WEVFTQGKPL PAELDFDKLE AVAQDAFPAA 490 500 510 520 530 540 LARASEYLTH PVFNTHHAEH EMLRYLRMLA DKDLALDRTM IPLGSCTMKL NATSEMIPVT 550 560 570 580 590 600 WPEFSQIHPF APLDQTVGYR EMIDQLEAML CAATGYAAVS LQPNAGSQGE YAGLLIIHAY 610 620 630 640 650 660 HASRGESHRD ICLIPSSAHG TNPASAQMAG MKVVVVACDE DGNVDLQDLA KKAEQHSKNL 670 680 690 700 710 720 AAIMITYPST HGVFEQGVQQ ICEIVHQHGG QVYVDGANMN AMVGTAAPGQ FGGDVSHLNL 730 740 750 760 770 780 HKTFCIPHGG GGPGVGPVAV GAHLADFLPN QDSVGYRRDD RGIGGVSAAP FGSASILPIS 790 800 810 820 830 840 WMYIAMMGSA GLTAATENAI LAANYVAKRL APYYPVLYTG QHDLVAHECI LDLRPLQKDT 850 860 870 880 890 900 GISNEDVAKR LMDYGFHAPT MSFPVPGTLM IEPTESEALH ELDRFIDAMI AIRQEIGRVA 910 920 930 940 950 960 DGTFDRDDNP LKHAPHTAAV VTADEWTHKY TREEAAYPVA SLRTQKYWPP VGRADNVYGD 970 RNLFCACVPV SDYVVD

References

[1]	"Complete genome sequence of the type strain Cupriavidus necator N-1." Poehlein A., Kusian B., Friedrich B., Daniel R., Bowien B. J. Bacteriol. 193:5017-5017(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43291 / DSM 13513 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002877 Genomic DNA. Translation: AEI78864.1.
RefSeq	YP_004687345.1. NC_015726.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEI78864; AEI78864; CNE_1c35720.
GeneID	10919963.
KEGG	cnc:CNE_1c35720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K00281.
Enzyme and pathway databases
BioCyc	CNEC1042878:GH0Z-4975-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 2 hits.
HAMAP	MF_00711. GcvP.
InterPro	IPR020580. GDC-P_N. IPR020581. GDC_P. IPR003437. GDC_P_homo. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11773. PTHR11773. 1 hit.
Pfam	PF02347. GDC-P. 2 hits. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 2 hits.
TIGRFAMs	TIGR00461. gcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G0F028_CUPNN

Accession

Primary (citable) accession number: G0F028

Entry history

Integrated into UniProtKB/TrEMBL:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)