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Protein

Glycine dehydrogenase (decarboxylating)

Gene

gcvP

Organism
Cupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.UniRule annotationSAAS annotation

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

GO - Molecular functioni

  1. glycine dehydrogenase (decarboxylating) activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine decarboxylation via glycine cleavage system Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCNEC1042878:GH0Z-3572-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine dehydrogenase (decarboxylating)UniRule annotation (EC:1.4.4.2UniRule annotation)
Alternative name(s):
Glycine cleavage system P-proteinUniRule annotation
Glycine decarboxylaseUniRule annotation
Glycine dehydrogenase (aminomethyl-transferring)UniRule annotation
Gene namesi
Name:gcvPUniRule annotationImported
Ordered Locus Names:CNE_1c35720Imported
OrganismiCupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha)Imported
Taxonomic identifieri1042878 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000006798: Chromosome 1

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei722 – 7221N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.UniRule annotationSAAS annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family.UniRule annotation

Phylogenomic databases

KOiK00281.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.

Sequencei

Sequence statusi: Complete.

G0F028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAPLPMNAA QVTRPTLAEL EARDAFAARH IGPDTPEQQH MLKVLGYDSR
60 70 80 90 100
AALIDAVIPA AIRRRDGMPM GEFTEPLSEE AALAKLRGLA GRNKVLKSFI
110 120 130 140 150
GQGYYNTVTP GVILRNIFEN PAWYTAYTPY QPEISQGRLE AMLNFQQMVT
160 170 180 190 200
DLTGLDIANA SMLDEGTAAA EAMTLLQRVN KHDSNTFYVA DDVLPQTLEV
210 220 230 240 250
VRTRALPMGI EVKVGPAAEA AAANAFGVLL QYPGVNGDVN DYGAIADAVH
260 270 280 290 300
AAGGLVVAAA DLLALTLIAA PGEWGADVAV GNSQRFGVPL GFGGPHAGYM
310 320 330 340 350
AVKDAFKRSM PGRLVGVTID AQGNKAYRLA LQTREQHIRR EKATSNICTA
360 370 380 390 400
QVLLAVMASM YAVYHGPQGL KRIAQRVHRL TATLAAGLQA LGFARTNASF
410 420 430 440 450
FDTLTLETGF NTDAIHAAAT ARGINLRHIS ATRVGISLDE TASRADVVAL
460 470 480 490 500
WEVFTQGKPL PAELDFDKLE AVAQDAFPAA LARASEYLTH PVFNTHHAEH
510 520 530 540 550
EMLRYLRMLA DKDLALDRTM IPLGSCTMKL NATSEMIPVT WPEFSQIHPF
560 570 580 590 600
APLDQTVGYR EMIDQLEAML CAATGYAAVS LQPNAGSQGE YAGLLIIHAY
610 620 630 640 650
HASRGESHRD ICLIPSSAHG TNPASAQMAG MKVVVVACDE DGNVDLQDLA
660 670 680 690 700
KKAEQHSKNL AAIMITYPST HGVFEQGVQQ ICEIVHQHGG QVYVDGANMN
710 720 730 740 750
AMVGTAAPGQ FGGDVSHLNL HKTFCIPHGG GGPGVGPVAV GAHLADFLPN
760 770 780 790 800
QDSVGYRRDD RGIGGVSAAP FGSASILPIS WMYIAMMGSA GLTAATENAI
810 820 830 840 850
LAANYVAKRL APYYPVLYTG QHDLVAHECI LDLRPLQKDT GISNEDVAKR
860 870 880 890 900
LMDYGFHAPT MSFPVPGTLM IEPTESEALH ELDRFIDAMI AIRQEIGRVA
910 920 930 940 950
DGTFDRDDNP LKHAPHTAAV VTADEWTHKY TREEAAYPVA SLRTQKYWPP
960 970
VGRADNVYGD RNLFCACVPV SDYVVD
Length:976
Mass (Da):104,999
Last modified:October 19, 2011 - v1
Checksum:iA9A9D4EC2E7B7DC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002877 Genomic DNA. Translation: AEI78864.1.
RefSeqiYP_004687345.1. NC_015726.1.

Genome annotation databases

EnsemblBacteriaiAEI78864; AEI78864; CNE_1c35720.
GeneIDi10919963.
KEGGicnc:CNE_1c35720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002877 Genomic DNA. Translation: AEI78864.1.
RefSeqiYP_004687345.1. NC_015726.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEI78864; AEI78864; CNE_1c35720.
GeneIDi10919963.
KEGGicnc:CNE_1c35720.

Phylogenomic databases

KOiK00281.

Enzyme and pathway databases

BioCyciCNEC1042878:GH0Z-3572-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the type strain Cupriavidus necator N-1."
    Poehlein A., Kusian B., Friedrich B., Daniel R., Bowien B.
    J. Bacteriol. 193:5017-5017(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43291 / DSM 13513 / N-1Imported.

Entry informationi

Entry nameiG0F028_CUPNN
AccessioniPrimary (citable) accession number: G0F028
Entry historyi
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: March 4, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.