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Protein
Submitted name:

Elongation factor Tu

Gene

tufB2

Organism
Cupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factorUniRule annotationImported

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCNEC1042878:GH0Z-3437-MONOMER.
CNEC1042878:GH0Z-3452-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Elongation factor TuImported
Gene namesi
Name:tufB2Imported
Synonyms:tufUniRule annotation, tufB1Imported
Ordered Locus Names:CNE_1c34370Imported, CNE_1c34520Imported
OrganismiCupriavidus necator (strain ATCC 43291 / DSM 13513 / N-1) (Ralstonia eutropha)Imported
Taxonomic identifieri1042878 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000006798 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiG0EZB4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliG0EZB4.
SMRiG0EZB4. Positions 2-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

KOiK02358.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G0EZB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEKFERTK PHVNVGTIGH VDHGKTTLTA AIATVLAAKF GGAAKKYDEI
60 70 80 90 100
DAAPEEKARG ITINTAHVEY ETANRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVCSAAD GPMPQTREHI LLARQVGVPY IIVFLNKCDM VDDAELLELV
160 170 180 190 200
EMEVRELLSK YEFPGDDTPI IKGSAKLALE GDKGELGEVA IMNLADALDT
210 220 230 240 250
YIPTPERAVD GTFLMPVEDV FSISGRGTVV TGRIERGVVK VGEEIEIVGI
260 270 280 290 300
KPTVKTTCTG VEMFRKLLDQ GQAGDNVGLL LRGTKREDVE RGQVLCKPGS
310 320 330 340 350
IKPHTHFTGE VYILSKDEGG RHTPFFNNYR PQFYFRTTDV TGSIELPKDK
360 370 380 390
EMVMPGDNVS ITVKLIAPIA MEEGLRFAIR EGGRTVGAGV VAKILD
Length:396
Mass (Da):43,046
Last modified:October 18, 2011 - v1
Checksum:i265C1E13A2E0163B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002877 Genomic DNA. Translation: AEI78740.1.
CP002877 Genomic DNA. Translation: AEI78754.1.
RefSeqiYP_004687221.1. NC_015726.1.
YP_004687235.1. NC_015726.1.

Genome annotation databases

EnsemblBacteriaiAEI78740; AEI78740; CNE_1c34370.
AEI78754; AEI78754; CNE_1c34520.
KEGGicnc:CNE_1c34370.
cnc:CNE_1c34520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002877 Genomic DNA. Translation: AEI78740.1.
CP002877 Genomic DNA. Translation: AEI78754.1.
RefSeqiYP_004687221.1. NC_015726.1.
YP_004687235.1. NC_015726.1.

3D structure databases

ProteinModelPortaliG0EZB4.
SMRiG0EZB4. Positions 2-395.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiG0EZB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEI78740; AEI78740; CNE_1c34370.
AEI78754; AEI78754; CNE_1c34520.
KEGGicnc:CNE_1c34370.
cnc:CNE_1c34520.

Phylogenomic databases

KOiK02358.

Enzyme and pathway databases

BioCyciCNEC1042878:GH0Z-3437-MONOMER.
CNEC1042878:GH0Z-3452-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the type strain Cupriavidus necator N-1."
    Poehlein A., Kusian B., Friedrich B., Daniel R., Bowien B.
    J. Bacteriol. 193:5017-5017(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43291 / DSM 13513 / N-1Imported and N-1Imported.

Entry informationi

Entry nameiG0EZB4_CUPNN
AccessioniPrimary (citable) accession number: G0EZB4
Entry historyi
Integrated into UniProtKB/TrEMBL: October 18, 2011
Last sequence update: October 18, 2011
Last modified: March 31, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.