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G0EPT1 (G0EPT1_BRAIP) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AEM20795.1
Ordered Locus Names:Bint_0161 EMBL AEM20795.1
OrganismBrachyspira intermedia (strain ATCC 51140 / PWS/A) (Serpulina intermedia) [Complete proteome] [HAMAP] EMBL AEM20795.1
Taxonomic identifier1045858 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain121 – 325205ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding152 – 20655ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2791Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2921Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2921Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2941Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G0EPT1 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: C6FB286F006526A9

FASTA32836,909
        10         20         30         40         50         60 
MILNSELKQN ILNRFKNKKI AVLHGGLSSE REVSLRSGQN VYKALTSFDE IKDNCILIDV 

        70         80         90        100        110        120 
KDSYELVKTL KENNIEYCYN ILHGTSGEDG TIQGLLESLN IKYTGENILV SAVCMNKVYT 

       130        140        150        160        170        180 
KRIWKSSNVS TADFMLLKDV KEINDNNVKG SLLSFNFPII LKPISDGSSV GVHLIKTKSE 

       190        200        210        220        230        240 
FENVVKDIKD IQNYFLEPYI KGKEITVGLV KQDDDNTYVF PILGINPKNE IYDYEAKYTP 

       250        260        270        280        290        300 
GKTEMEMPAK LSKEMENKVV ETCKKAYRVL GCSGLSRIDA IVGDDNEVYL MEVNTQGGMT 

       310        320 
NTSDIPAMAK HINLDFNDLV LYILGLLK 

« Hide

References

[1]"Complete genome sequence of Brachyspira intermedia reveals unique genomic features in Brachyspira species and phage-mediated horizontal gene transfer."
Hafstrom T., Jansson D.S., Segerman B.
BMC Genomics 12:395-395(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51140 / PWS/A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002874 Genomic DNA. Translation: AEM20795.1.
RefSeqYP_005593375.1. NC_017243.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEM20795; AEM20795; Bint_0161.
GeneID12157578.
KEGGbip:Bint_0161.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMAMEFCVLF.

Enzyme and pathway databases

BioCycBINT1045858:GL9O-161-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG0EPT1_BRAIP
AccessionPrimary (citable) accession number: G0EPT1
Entry history
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: February 19, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)