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G0EGF8 (G0EGF8_PYRF1) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00284
EC=6.1.1.20 HAMAP-Rule MF_00284
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit HAMAP-Rule MF_00284
Gene names
Name:pheT HAMAP-Rule MF_00284
Ordered Locus Names:Pyrfu_1324 EMBL AEM39183.1
OrganismPyrolobus fumarii (strain DSM 11204 / 1A) [Complete proteome] [HAMAP]
Taxonomic identifier694429 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Pyrolobus ›

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00284

Subunit structure

Tetramer of two alpha and two beta subunits By similarity. HAMAP-Rule MF_00284

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00284.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily. HAMAP-Rule MF_00284

Contains 1 B5 domain. HAMAP-Rule MF_00284

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain273 – 34876B5 By similarity HAMAP-Rule MF_00284

Sites

Metal binding3261Magnesium By similarity HAMAP-Rule MF_00284
Metal binding3321Magnesium; via carbonyl oxygen By similarity HAMAP-Rule MF_00284
Metal binding3351Magnesium By similarity HAMAP-Rule MF_00284
Metal binding3361Magnesium By similarity HAMAP-Rule MF_00284

Sequences

Sequence LengthMass (Da)Tools
G0EGF8 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: 993B4124AE48F2D7

FASTA56363,015
        10         20         30         40         50         60 
MPTLRFLAPR LFEAIGSSVT RQELEELLFR LKCETEWGEG DEVYVEVNAD RPDMFSVEGI 

        70         80         90        100        110        120 
ARAVRGLLGI ETGLPSYEHV PSGFTLRVES VPSRPFIAAG VVYDVYVDGV FLEELIQFQE 

       130        140        150        160        170        180 
KLHETLGRGR RRVAIGIHDL EKVPSKNLVY REVNIDEVRF TPLHGDREMS VREILAETEQ 

       190        200        210        220        230        240 
GRKYGAISLR GDRHPVFATE DGVVLSLPPV INADVTRVEP GTRHLLIDVT GTSWEAVKQV 

       250        260        270        280        290        300 
LNVLVYTLAE RSRSKRVGIV RLVGAPESET PVWSTRSITA SHERIVAWLG ARLEPSDVVD 

       310        320        330        340        350        360 
ALERMRHNAE YDAGAYTVEA APYRVDVSTW VDVAEDVAIG IGYDRVGWQA PPPTTRGSLS 

       370        380        390        400        410        420 
PIRGLERSIR VILAGYGFQE VYSFTLTSCE RQEELGAVPT TMLVRVANPV SRELACLRAS 

       430        440        450        460        470        480 
ITPQLLELAA QNQHIVPLRI FEMGDVALVD ESRPEKVRIE KHLAILLMDE RVGYEDIQSY 

       490        500        510        520        530        540 
VWGLVRLLGD EIREVEEVDH PLLIPGRAAR IKTRDGLEGI LGEVHPAHLE KLEIRYPVAI 

       550        560 
AELRYDTVAK PRLANPYKAL HSS

« Hide

References

[1]"Complete genome sequence of the hyperthermophilic chemolithoautotroph Pyrolobus fumarii type strain (1A)."
Anderson I., Goker M., Nolan M., Lucas S., Hammon N., Deshpande S., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Huntemann M., Liolios K., Ivanova N., Pagani I., Mavromatis K., Ovchinikova G., Pati A. expand/collapse author list , Chen A., Palaniappan K., Land M., Hauser L., Brambilla E.M., Huber H., Yasawong M., Rohde M., Spring S., Abt B., Sikorski J., Wirth R., Detter J.C., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P., Lapidus A.
Stand. Genomic Sci. 4:381-392(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 11204 / 1A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002838 Genomic DNA. Translation: AEM39183.1.
RefSeqYP_004781435.1. NC_015931.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEM39183; AEM39183; Pyrfu_1324.
GeneID11138507.
KEGGpfm:Pyrfu_1324.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01890.
OMAFFPNRPD.

Enzyme and pathway databases

BioCycPFUM694429:GHUY-1365-MONOMER.

Family and domain databases

Gene3D3.30.56.20. 1 hit.
HAMAPMF_00284. Phe_tRNA_synth_beta2.
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR022918. Phe_tRNA_ligase_beta2_bac/arc.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMSSF46955. Putativ_DNA_bind. 2 hits.
TIGRFAMsTIGR00471. pheT_arch. 1 hit.
PROSITEPS51483. B5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG0EGF8_PYRF1
AccessionPrimary (citable) accession number: G0EGF8
Entry history
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info