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Protein

Fructose-1,6-bisphosphate aldolase/phosphatase

Gene

fbp

Organism
Pyrolobus fumarii (strain DSM 11204 / 1A)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).UniRule annotation

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.UniRule annotation
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34Proton acceptor; for FBP phosphatase activityUniRule annotation1
Metal bindingi34Magnesium 1UniRule annotation1
Metal bindingi41Magnesium 1; via pros nitrogenUniRule annotation1
Binding sitei41FBP; via tele nitrogenUniRule annotation1
Metal bindingi75Magnesium 1UniRule annotation1
Metal bindingi75Magnesium 2UniRule annotation1
Metal bindingi76Magnesium 2UniRule annotation1
Binding sitei113FBPUniRule annotation1
Metal bindingi117Magnesium 1UniRule annotation1
Metal bindingi154Magnesium 2UniRule annotation1
Binding sitei155FBP/DHAPUniRule annotation1
Active sitei251Proton donor/acceptor; for FBP aldolase activityUniRule annotation1
Active sitei254Schiff-base intermediate with DHAP; for FBP aldolase activityUniRule annotation1
Metal bindingi254Magnesium 3; via carbonyl oxygenUniRule annotation1
Metal bindingi255Magnesium 3UniRule annotation1
Metal bindingi255Magnesium 4UniRule annotation1
Metal bindingi256Magnesium 2UniRule annotation1
Metal bindingi256Magnesium 3UniRule annotation1
Binding sitei288FBP/DHAP; via amide nitrogenUniRule annotation1
Binding sitei309FBP/DHAPUniRule annotation1
Binding sitei370FBPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation, LyaseUniRule annotation
Biological processCarbohydrate metabolismUniRule annotation, GluconeogenesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, Schiff baseUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphate aldolase/phosphataseUniRule annotation (EC:3.1.3.11UniRule annotation, EC:4.1.2.13UniRule annotation)
Short name:
FBP A/PUniRule annotation
Short name:
FBP aldolase/phosphataseUniRule annotation
Gene namesi
Name:fbpUniRule annotation
Ordered Locus Names:Pyrfu_1765Imported
OrganismiPyrolobus fumarii (strain DSM 11204 / 1A)Imported
Taxonomic identifieri694429 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaePyrolobus
Proteomesi
  • UP000001037 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; dimer of tetramers.UniRule annotation

Protein-protein interaction databases

STRINGi694429.Pyrfu_1765.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 127FBP bindingUniRule annotation2
Regioni264 – 265FBP binding; shared with dimeric partnerUniRule annotation2

Domaini

Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.UniRule annotation

Sequence similaritiesi

Belongs to the FBP aldolase/phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.

Sequencei

Sequence statusi: Complete.

G0ECP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCVRKPVYPT EHVYTMVQVM SEERKVTISV IKADIGSIAG HHKVHPDTMA
60 70 80 90 100
AAARVLAEAK RKGVIIDFYV THVGDDLQLI MTHRKGVDSP EIHELAWNAF
110 120 130 140 150
KKAAEVAKEL GLYAAGQDLL SDAFSGNIRG LGPGVAEMEI VERPSEPIVI
160 170 180 190 200
FMADKTEPGA FNLPLFRAFA DPFTTAGLVI DPRMHDGFRF QILDLYEGRM
210 220 230 240 250
IELNAPEEMY DILALIGDPT RYVIKRIFRK TDGEIAAVVS SERLNLIAGR
260 270 280 290 300
YVGKDDPVAI ARVQHGFPAL GEILEPFAFP HLVPGWMRGS HWGPLMPVGL
310 320 330 340 350
RDARCTRFDG PPRVIALGFQ VTKGRLIGPV DLFEDVAFDE TRRLAQQIAE
360 370 380 390 400
YMRRHGPFMP HRLHAEEMEY TTLPDVLKKL EGRWKKVEGP EAREIGKRER

KESAEVQE
Length:408
Mass (Da):45,780
Last modified:October 19, 2011 - v1
Checksum:iD705F44A19529E7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002838 Genomic DNA. Translation: AEM39619.1.

Genome annotation databases

EnsemblBacteriaiAEM39619; AEM39619; Pyrfu_1765.
KEGGipfm:Pyrfu_1765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002838 Genomic DNA. Translation: AEM39619.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi694429.Pyrfu_1765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEM39619; AEM39619; Pyrfu_1765.
KEGGipfm:Pyrfu_1765.

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG0ECP9_PYRF1
AccessioniPrimary (citable) accession number: G0ECP9
Entry historyiIntegrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: May 10, 2017
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.