Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

G0E9Z8 (G0E9Z8_ENTAK) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_00206

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity SAAS SAAS003698 HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_00206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding681Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding731Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding791Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding981Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding1011Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206

Sequences

Sequence LengthMass (Da)Tools
G0E9Z8 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: 702517F955625D49

FASTA32136,162
        10         20         30         40         50         60 
MSKPIVMERG VKYRDADKMA LIPVKNVATE REALLRKPEW MKIKLPADSS RIQGIKAAMR 

        70         80         90        100        110        120 
KNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPVT PDANEPQKLA 

       130        140        150        160        170        180 
QTIADMGLRY VVVTSVDRDD LRDGGAQHFA DCISAIREKS PNIKIETLVP DFRGRMDRAL 

       190        200        210        220        230        240 
DILKVTPPDV FNHNLENVPR LYRQVRPGAD YNWSLKLLER FKEAHPEIPT KSGLMVGLGE 

       250        260        270        280        290        300 
TNEEIIEVMR DLRRHGVTML TLGQYLQPSR HHLPVQRYVS PEEFEEMKAE AMAMGFTHAA 

       310        320 
CGPFVRSSYH ADLQAKGMEV K 

« Hide

References

[1]"Complete sequence of Enterobacter aerogenes KCTC 2190."
Shin S.H., Kim S., Kim J.Y., Yang K.-S., Seo J.-S.
Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002824 Genomic DNA. Translation: AEG97679.1.
RefSeqYP_004592958.1. NC_015663.1.

3D structure databases

ProteinModelPortalG0E9Z8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEG97679; AEG97679; EAE_13825.
GeneID10792976.
KEGGeae:EAE_13825.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03644.
OMAHPHIPTK.

Enzyme and pathway databases

BioCycEAER1028307:GHNA-2818-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameG0E9Z8_ENTAK
AccessionPrimary (citable) accession number: G0E9Z8
Entry history
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: June 11, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)