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Protein

Ribosomal RNA large subunit methyltransferase K/L

Gene

rlmL

Organism
Enterobacter aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006) (Aerobacter aerogenes)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA.UniRule annotationSAAS annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA.UniRule annotationSAAS annotation
S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA.UniRule annotationSAAS annotation

GO - Molecular functioni

  1. 23S rRNA (guanine(2445)-N(2))-methyltransferase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: UniProtKB-HAMAP
  3. rRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

rRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

RNA-bindingUniRule annotationSAAS annotation, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciEAER1028307:GHNA-3125-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase K/LUniRule annotationSAAS annotation
Gene namesi
Name:rlmLUniRule annotationImported
Ordered Locus Names:EAE_15305Imported
OrganismiEnterobacter aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006) (Aerobacter aerogenes)Imported
Taxonomic identifieri1028307 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
ProteomesiUP000008881 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 154112THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RlmKL family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

KOiK12297.
OMAiGYEADPR.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_01858. 23SrRNA_methyltr_KL.
InterProiIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP.
[Graphical view]
PfamiPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFiPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
PS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G0DZL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLFASTAR GLEELLKTEL EGLGATACQV VQGGVHFQGD TRLLYQSLMW
60 70 80 90 100
SRLASRIMLP LGECSVYSDL DLYLGVQSIP WTEIFTTDAT FAVHFSGLNE
110 120 130 140 150
EIRNSQYGAL KVKDAIVDSF TRKNLPRPNV DRENPDLRIN VWLNKETAHI
160 170 180 190 200
SLDLSGEGLH LRGYRDGTGM APIKENLAAA IVMRSGWVAG TPLLDPMCGS
210 220 230 240 250
GTLLIEAAML ATDRAPGLHR GHWGFGGWAQ HDDAIWKEVK AEAQTRARQG
260 270 280 290 300
LAAYESRFYG SDVDERVIER ARRNARRAGI GELINFEVKD VAQLNNPLPK
310 320 330 340 350
GPYGTVISNP PYGERLESEP ALIALHSLLG RIMKNQFGGW NLSVFSASPE
360 370 380 390 400
LLSCLQLRAD KQFKAKNGPL DCVQKNYHLA ESEGGKPAML AEDFANRLRK
410 420 430 440 450
NLKKFEKWAR QEGIECYRLY DADLPEYNVA IDRYGEWVVV QEYAPPKTVD
460 470 480 490 500
AHKARQRLFD IIAATIAVLG IAPNKLVLKT RERQKGKNQY QKMAEKGDFI
510 520 530 540 550
EVQEYNARLW VNLTDYLDTG LFLDHRIARC MLGQMSKGKD FLNLFSYTGS
560 570 580 590 600
ASVHAGLGGA RSTTTVDMSR TYLEWAERNL RLNGLTGRAH RLMQADVLGW
610 620 630 640 650
LRESTEQFDL IFIDPPTFSN SKRMEDAFDV QRDHIRLMTD LKRLLRKGGT
660 670 680 690 700
IMFSNNKRGF RMDHDGLAAL GLKAQEISQK TLSQDFARNR QIHNCWLITA

A
Length:701
Mass (Da):78,843
Last modified:October 19, 2011 - v1
Checksum:iA912936E9126B028
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002824 Genomic DNA. Translation: AEG97974.1.
RefSeqiWP_015704903.1. NC_015663.1.
YP_004593253.1. NC_015663.1.

Genome annotation databases

EnsemblBacteriaiAEG97974; AEG97974; EAE_15305.
GeneIDi10793283.
KEGGieae:EAE_15305.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002824 Genomic DNA. Translation: AEG97974.1.
RefSeqiWP_015704903.1. NC_015663.1.
YP_004593253.1. NC_015663.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEG97974; AEG97974; EAE_15305.
GeneIDi10793283.
KEGGieae:EAE_15305.

Phylogenomic databases

KOiK12297.
OMAiGYEADPR.

Enzyme and pathway databases

BioCyciEAER1028307:GHNA-3125-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_01858. 23SrRNA_methyltr_KL.
InterProiIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR029063. SAM-dependent_MTases.
IPR004114. THUMP.
[Graphical view]
PfamiPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFiPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
PS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Enterobacter aerogenes KCTC 2190."
    Shin S.H., Kim S., Kim J.Y., Yang K.-S., Seo J.-S.
    Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006Imported.

Entry informationi

Entry nameiG0DZL2_ENTAK
AccessioniPrimary (citable) accession number: G0DZL2
Entry historyi
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: April 29, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.