G0BNY9 (G0BNY9_9ENTR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

ORF Names:

SerAS12_0691 EMBL AEF48798.1

Organism

Serratia sp. AS12 EMBL AEF48798.1

Taxonomic identifier

768490 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630 EMBL AEF48798.1
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	G0BNY9 [UniParc]. Last modified October 19, 2011. Version 1. Checksum: 73259A8660255CBE	FASTA	453	47,349
	10 20 30 40 50 60 MIPVSLQALA DVLSAELIGA DCQIIEVTTD TRQVTAGCLF VALKGERFDA HAFAADAVAA 70 80 90 100 110 120 GAGALLVSKR LLVDVPQLVV KDTRLALGQL AAWVRQQVPA RVVALTGSSG KTSVKEMVAA 130 140 150 160 170 180 ILRECGEVLY TAGNFNNDIG VPLTLLRLQP QHDFAVIELG ANHIGEIAYT TALTRPQTAL 190 200 210 220 230 240 VNNLAAAHLE GFGSLAGVAQ AKGEIFTGLP ADGVAIINAD NNDWPHWQSM LNGKTVWRFS 250 260 270 280 290 300 PQAAEGVDFF ASDVLVNAKG TQFTLHSPFG TAVVTLPLPG RHNVANALAA AALAMSVGAS 310 320 330 340 350 360 LESVRQGLKQ LQGVPGRLFP IALTENKLLL DDSYNANVGS MTAAAQVLAE MPGYRVMVVG 370 380 390 400 410 420 DMAELGADAE ECHRQVGEAA RLAGIDKVIG IGPLSQALCA ASGNGEHFQN KAAVIARVAE 430 440 450 LLSEHAVITV LIKGSRSAAM EQVVRALQEK APC

References

[1]

"Complete sequence of Serratia sp. AS12." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Chertkov O., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Neupane S., Alstrom S. , Finlay R., Hogberg N., Woyke T. Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: AS12 EMBL AEF48798.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002774 Genomic DNA. Translation: AEF48798.1.
RefSeq	YP_004499155.1. NC_015566.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEF48798; AEF48798; SerAS12_0691.
GeneID	10624883.
KEGG	srs:SerAS12_0691.
Phylogenomic databases
KO	K01929.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. murF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G0BNY9_9ENTR

Accession

Primary (citable) accession number: G0BNY9

Entry history

Integrated into UniProtKB/TrEMBL:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)