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G0BL75 (G0BL75_9ENTR) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 8. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase B HAMAP-Rule MF_01080

EC=5.4.99.25 HAMAP-Rule MF_01080
Alternative name(s):
tRNA pseudouridine(55) synthase HAMAP-Rule MF_01080
tRNA pseudouridylate synthase HAMAP-Rule MF_01080
tRNA-uridine isomerase HAMAP-Rule MF_01080
Gene names
Name:truB HAMAP-Rule MF_01080
ORF Names:SerAS12_0431 EMBL AEF48539.1
OrganismSerratia sp. AS12 EMBL AEF48539.1
Taxonomic identifier768490 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080 SAAS SAAS014780

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080 SAAS SAAS014780

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. HAMAP-Rule MF_01080

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site481Nucleophile By similarity HAMAP-Rule MF_01080
Binding site431Substrate By similarity HAMAP-Rule MF_01080
Binding site761Substrate By similarity HAMAP-Rule MF_01080
Binding site1791Substrate By similarity HAMAP-Rule MF_01080
Binding site2001Substrate By similarity HAMAP-Rule MF_01080

Sequences

Sequence LengthMass (Da)Tools
G0BL75 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: 35269DF87BEF8B6F

FASTA31435,108
        10         20         30         40         50         60 
MSRPRRRGRD IHGVLLLDKP QGLSSNDALQ KVKRLYNANR AGHTGALDPL ATGMLPICLG 

        70         80         90        100        110        120 
EATKFSQFLL DSDKRYRVIA KLGQRTDTSD ADGQIVQERP VNFTQTQLDT ALDSFRGDIK 

       130        140        150        160        170        180 
QVPSMYSALK YQGKKLYEYA RQGIEVPREA RSITVYELQF IRWEGDELEL EIHCSKGTYI 

       190        200        210        220        230        240 
RTITDDLGEL LGCGAHVIYL RRLQVATYPI ERMVTLEQLN ALLEQAQARE IAPGELLDPL 

       250        260        270        280        290        300 
LMPMDSPVEN YPEVNLLPVV AGYVKQGQPV QVAGAPASGM VRMTEGEERK FIGVGDIADD 

       310 
GRVAPRRLVV EHFD 

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References

[1]"Complete sequence of Serratia sp. AS12."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Chertkov O., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Neupane S., Alstrom S. expand/collapse author list , Finlay R., Hogberg N., Woyke T.
Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: AS12 EMBL AEF48539.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002774 Genomic DNA. Translation: AEF48539.1.
RefSeqYP_004498896.1. NC_015566.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEF48539; AEF48539; SerAS12_0431.
GeneID10624624.
KEGGsrs:SerAS12_0431.

Phylogenomic databases

KOK03177.

Family and domain databases

HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00431. TruB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameG0BL75_9ENTR
AccessionPrimary (citable) accession number: G0BL75
Entry history
Integrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: April 3, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)