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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Methylomonas methanica (strain MC09)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.UniRule annotation

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1UniRule annotation (EC:4.1.3.27UniRule annotation)
Gene namesi
Name:trpEUniRule annotation
Ordered Locus Names:Metme_4596Imported
OrganismiMethylomonas methanica (strain MC09)Imported
Taxonomic identifieri857087 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylomonas
Proteomesi
  • UP000008888 Componenti: Chromosome

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).UniRule annotation

Protein-protein interaction databases

STRINGi857087.Metme_4596.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 168Anth_synt_I_NInterPro annotationAdd BLAST143
Domaini222 – 475Chorismate_bindInterPro annotationAdd BLAST254

Sequence similaritiesi

Belongs to the anthranilate synthase component I family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
KOiK01657.
OMAiCEELAVI.
OrthoDBiPOG091H01VQ.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
PfamiView protein in Pfam
PF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.

Sequencei

Sequence statusi: Complete.

G0A6E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPAQFTDYA QQGYNRIPVC REVLADLDTP LSAYLKLADG AYSYLFESVH
60 70 80 90 100
GGEQWGRYSI IGLPCKTRVK ISGNTISLEQ DGADTQTFEH AQPLEWIEEF
110 120 130 140 150
RQQYQVPDIE GLPRFNGGLV GYFGYETIGY IEPRLKSAGK PDPIGTPDIL
160 170 180 190 200
LMVSQDLLVF DNLSGKMLLL THADPAQEGA YELAQARLAE LVDKLHQLKA
210 220 230 240 250
HPQPHPAVKQ VHENDFVSGF TQQGFEDAVR KAKQYITDGD VMQVVLSQRM
260 270 280 290 300
SIPFSASPLD LYRSLRCLNP SPYMYYLNLD GFHIVGSSPE ILVRLEDDEV
310 320 330 340 350
TVRPIAGTRP RGATHEQDQA LEQELLADPK EIAEHLMLID LGRNDAGRVA
360 370 380 390 400
KIGSVKLTDK MIIERYSHVM HIVSNVTGQL QDGKNAFDVL AATFPAGTVS
410 420 430 440 450
GAPKIRAMEI IDELEPVKRG IYSGAVGYIS WSGNLDTAIA IRTAVIKDQT
460 470 480 490
LHIQAGAGIV YDSVPRNEWD ETMNKGRAVF RAVSMAEAGL GGKA
Length:494
Mass (Da):54,501
Last modified:October 19, 2011 - v1
Checksum:i1F00CDAC48A83D8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002738 Genomic DNA. Translation: AEG02934.1.
RefSeqiWP_013821147.1. NC_015572.1.

Genome annotation databases

EnsemblBacteriaiAEG02934; AEG02934; Metme_4596.
KEGGimmt:Metme_4596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002738 Genomic DNA. Translation: AEG02934.1.
RefSeqiWP_013821147.1. NC_015572.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi857087.Metme_4596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEG02934; AEG02934; Metme_4596.
KEGGimmt:Metme_4596.

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
KOiK01657.
OMAiCEELAVI.
OrthoDBiPOG091H01VQ.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
PfamiView protein in Pfam
PF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG0A6E9_METMM
AccessioniPrimary (citable) accession number: G0A6E9
Entry historyiIntegrated into UniProtKB/TrEMBL: October 19, 2011
Last sequence update: October 19, 2011
Last modified: May 10, 2017
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.