ID   G0A550_METMM            Unreviewed;      1044 AA.
AC   G0A550;
DT   19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT   19-OCT-2011, sequence version 1.
DT   27-MAR-2024, entry version 57.
DE   RecName: Full=Type I restriction enzyme endonuclease subunit {ECO:0000256|RuleBase:RU364115};
DE            Short=R protein {ECO:0000256|RuleBase:RU364115};
DE            EC=3.1.21.3 {ECO:0000256|RuleBase:RU364115};
GN   OrderedLocusNames=Metme_1967 {ECO:0000313|EMBL:AEG00380.1};
OS   Methylomonas methanica (strain MC09).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylomonas.
OX   NCBI_TaxID=857087 {ECO:0000313|EMBL:AEG00380.1, ECO:0000313|Proteomes:UP000008888};
RN   [1] {ECO:0000313|EMBL:AEG00380.1, ECO:0000313|Proteomes:UP000008888}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC09 {ECO:0000313|EMBL:AEG00380.1,
RC   ECO:0000313|Proteomes:UP000008888};
RX   PubMed=22123758; DOI=10.1128/JB.06267-11;
RA   Boden R., Cunliffe M., Scanlan J., Moussard H., Kits K.D., Klotz M.G.,
RA   Jetten M.S., Vuilleumier S., Han J., Peters L., Mikhailova N., Teshima H.,
RA   Tapia R., Kyrpides N., Ivanova N., Pagani I., Cheng J.F., Goodwin L.,
RA   Han C., Hauser L., Land M.L., Lapidus A., Lucas S., Pitluck S., Woyke T.,
RA   Stein L., Murrell J.C.;
RT   "Complete Genome Sequence of the Aerobic Marine Methanotroph Methylomonas
RT   methanica MC09.";
RL   J. Bacteriol. 193:7001-7002(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=MC09;
RA   Boden R., Cunliffe M., Scanlan J., Moussard H., Kits K.D., Klotz M.,
RA   Jetten M., Vuilleumier S., Han J., Peters L., Mikhailova N., Teshima H.,
RA   Tapia R., Kyrpides N., Ivanova N., Pagani I., Cheng J.-F., Goodwin L.,
RA   Han C., Hauser L., Land M., Lapidus A., Lucas S., Pitluck S., Woyke T.,
RA   Stein L.Y., Murrell C.;
RT   "Complete genome sequence of the aerobic marine methanotroph Methylomonas
RT   methanica MC09.";
RL   Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|Proteomes:UP000008888}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC09 {ECO:0000313|Proteomes:UP000008888};
RA   Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Peters L., Mikhailova N., Teshima H., Han C., Tapia R., Land M., Hauser L.,
RA   Kyrpides N., Ivanova N., Pagani I., Stein L., Woyke T.;
RT   "Complete sequence of Methylomonas methanica MC09.";
RL   Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Subunit R is required for both nuclease and ATPase
CC       activities, but not for modification. {ECO:0000256|RuleBase:RU364115}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC         fragments with terminal 5'-phosphates, ATP is simultaneously
CC         hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851,
CC         ECO:0000256|RuleBase:RU364115};
CC   -!- SUBUNIT: The type I restriction/modification system is composed of
CC       three polypeptides R, M and S. {ECO:0000256|RuleBase:RU364115}.
CC   -!- SIMILARITY: Belongs to the HsdR family. {ECO:0000256|ARBA:ARBA00008598,
CC       ECO:0000256|RuleBase:RU364115}.
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DR   EMBL; CP002738; AEG00380.1; -; Genomic_DNA.
DR   RefSeq; WP_013818627.1; NC_015572.1.
DR   AlphaFoldDB; G0A550; -.
DR   STRING; 857087.Metme_1967; -.
DR   REBASE; 36271; Mme9ORF1963P.
DR   KEGG; mmt:Metme_1967; -.
DR   eggNOG; COG0610; Bacteria.
DR   HOGENOM; CLU_005762_1_0_6; -.
DR   OrthoDB; 9758243at2; -.
DR   Proteomes; UP000008888; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   CDD; cd18030; DEXHc_RE_I_HsdR; 1.
DR   CDD; cd22332; HsdR_N; 1.
DR   CDD; cd18800; SF2_C_EcoR124I-like; 1.
DR   Gene3D; 3.90.1570.50; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR   InterPro; IPR004473; Restrct_endonuc_typeI_HsdR.
DR   InterPro; IPR040980; SWI2_SNF2.
DR   InterPro; IPR021810; T1RH-like_C.
DR   NCBIfam; TIGR00348; hsdR; 1.
DR   PANTHER; PTHR30195:SF15; TYPE I RESTRICTION ENYME HINDI ENDONUCLEASE SUBUNIT; 1.
DR   PANTHER; PTHR30195; TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R; 1.
DR   Pfam; PF04313; HSDR_N; 1.
DR   Pfam; PF18766; SWI2_SNF2; 1.
DR   Pfam; PF11867; T1RH-like_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364115};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU364115};
KW   Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364115};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU364115};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008888};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747,
KW   ECO:0000256|RuleBase:RU364115}.
FT   DOMAIN          282..483
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51192"
SQ   SEQUENCE   1044 AA;  117250 MW;  925220AE41C6FD4C CRC64;
     MTEDQLEQEA LGWLAEVGYS TLYGPDLAPD GDNPERDNYL QVVLVERLRS AIARLNPSIP
     LAAREDALQQ ILNLEVPGLL AANRHFHRLL VNGVPVEYQK DGDTRGDRVR LIEFAEPSSN
     EWLAINQFAI KGPKHSRRPD IILFINGLPL VLLELKNPAD ENADIWKAFG QIQTYKEQIP
     DVFQYNEVLV VSDGSEARLG SLSSDAERFM QWRTIDGITL DPLGQFNELE TLIHGLLNPA
     TLLDYLRFFV LFEDDGGLVK KVAGYHQYHA VRAAIQQVVT ASRPDGNHKG GVVWHTQGSG
     KSITMTCFAA RVMQETAMEN PTIVVITDRN DLDGQLFGVF SLGQDLLREQ PVQAETRQDL
     RAKLDNRPSG GIVFATIQKF MPGADEDSFP VLSDRSNIVV IADEAHRTQY GFEAKLKTVK
     TQQTAANEGR VDMAAEYRGM YQVGYAQHLR DALPNATFVA FTGTPVSLED RDTRAVFGEY
     IHVYDMQQAK EDGATVAIYF ESRLAKLALK PEQLPQIDDE VDELAEDEEE DQQAKLKSRW
     AALEKVVGAE PRIASVAADL VAHFEERNKA QDGKAMVVAM SREICVHLYN EIIKLRPDWH
     SDDPEQGAIK IVMTGSASDK ALLRPHIYSK QQKKRLEKRF KDPADPLRLV IVRDMWLTGF
     DAPCVHTLYV DKPMKGHNLM QAIARVNRVF KDKQGGLVVD YIGIANELKS ALKDYTASQG
     RGRPTVDAAE AYAVLEEKLD ILRGILHGYD YSDFLTTGHK RLAGAANHVL GQKDGKKRFA
     DTSLAMSKAF TLCCTLDEAK AVREEMAFFQ AVKVILTKPD ISRKKRSDEE RDLAIRQIIN
     SAVVSEDVVD IFEAVGLDKP NIGILDEAFL ADVRNLPERN LAVELLERLL EGEIKSRFAG
     NVVQEKKFSE LLTNVIKRYQ NRAIETAQVI EELIQMAKKF REAAGRGESL GLSEDEIRFY
     DALADNESAV RELSDDTLKL IAHELTENLR QNITVDWSQR ESVRAKLRLM VKRILRKYKY
     PPDQQEAAIE LVLQQAQALG DAWI
//