F8WQD0 (SHS1_ASTTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shionone synthase
Short name=AtaSHS
EC=5.4.99.46

Gene names

Name:	SHS1
Synonyms:	ZW1

Organism

Aster tataricus (Tartarian aster)

Taxonomic identifier

588669 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Astereae › Australasian lineages › Aster

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidosqualene cyclase involved in the production of shionone, a triterpenoid with a rare 6/6/6/6 tetracyclic skeleton and a C-3 carbonyl carbon. Produces also small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = shionone. Ref.1
Tissue specificity	Expressed in stems, leaves and flowers. Lower expression in roots, stolons, rhizomes and radical leaves. Ref.1
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 4 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	triterpenoid biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	oxidosqualene cyclase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	Shionone synthase		PRO_0000418917
Regions
	Repeat	148 – 189	42	PFTB 1
	Repeat	513 – 555	43	PFTB 2
	Repeat	590 – 630	41	PFTB 3
	Repeat	639 – 680	42	PFTB 4

Sequences

	Sequence		Length	Mass (Da)
	F8WQD0 [UniParc]. Last modified October 19, 2011. Version 1. Checksum: A8404790E57BBC90	FASTA	761	87,565
	10 20 30 40 50 60 MWRLKIADGG NNPYLYSTNN FIGRQTWEFD PNYGTPEERD EVEQARLHFW NHRHEIKPSG 70 80 90 100 110 120 DTLWRMQFIR EKKFKQTIPQ VKIEDDEEIS YDKVTATMRR SVHLLEALLA DDGHWPAENS 130 140 150 160 170 180 GPSFFIQPLV MCLYITGHLN SVFPAEHRKE ILRYVYSHQN KDGGWGLHME GHSIMFGTTL 190 200 210 220 230 240 SYICMRLLGE GPDGGLNGAC TRARKWILDH GGAIANPSWG KVWLSILGVH EWVGCNPLPP 250 260 270 280 290 300 EFWLFPSFLP MSPGKMWSYC RLVFMPMSYL YGRRFVGPIT PLVLQLRKEL YAQPYNDIKW 310 320 330 340 350 360 KSSRHVCAKE DIYYPHPLLQ DLMWDSLYIL TEPLLTRWPF NKLRKKALAT TMRHIHYEDE 370 380 390 400 410 420 NSRYITIGSV EKILCMLACW DEDPNGVCFK KHLARIPDYI WVAEDGMKMQ TFGSQVWDAS 430 440 450 460 470 480 IGIQALLATE LTHDIAPILK KGHEFIKASQ VRDNPSGDFK SMYRHISKGS WTFSDQDHGW 490 500 510 520 530 540 QLSDCTTIGL TCCLLLSTMP PETVGEKMDP EQLKDAVNVI LSLQSENGGL AAWEPAGSSN 550 560 570 580 590 600 WLEMLNPIEF IEDIVIEHEY VECTGTGMEA LVLFKKLYPK HRTKEVESFL TNAARYLDNT 610 620 630 640 650 660 QMPDGSWYGE WGICFTYGTY YALGGLAAIE KTYENCQSIR KAVRFLLKTQ GEDGGWGESY 670 680 690 700 710 720 RSCAEKIYIP LDGNRSTVVH TAWAMLGLMH SKQEERDPIP LHRAAKLLIN SQMENGDFPQ 730 740 750 760 QDTTGAFKKN CLLHYPMYRN IYTLWALAQY RKKVLRQPTG I

References

[1]

"Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus." Sawai S., Uchiyama H., Mizuno S., Aoki T., Akashi T., Ayabe S., Takahashi T. FEBS Lett. 585:1031-1036(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB609123 mRNA. Translation: BAK52535.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 3 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SHS1_ASTTA

Accession

Primary (citable) accession number: F8WQD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	October 19, 2011
Last modified:	October 3, 2012
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program