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Protein

Shionone synthase

Gene

SHS1

Organism
Aster tataricus (Tartarian aster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Oxidosqualene cyclase involved in the production of shionone, a triterpenoid with a rare 6/6/6/6 tetracyclic skeleton and a C-3 carbonyl carbon. Produces also small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = shionone.1 Publication

GO - Molecular functioni

  1. oxidosqualene cyclase activity Source: UniProtKB

GO - Biological processi

  1. triterpenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Shionone synthase (EC:5.4.99.46)
Short name:
AtaSHS
Gene namesi
Name:SHS1
Synonyms:ZW1
OrganismiAster tataricus (Tartarian aster)
Taxonomic identifieri588669 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeAstereaeAustralasian lineagesAster

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 761761Shionone synthasePRO_0000418917Add
BLAST

Expressioni

Tissue specificityi

Expressed in stems, leaves and flowers. Lower expression in roots, stolons, rhizomes and radical leaves.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati148 – 18942PFTB 1Add
BLAST
Repeati513 – 55543PFTB 2Add
BLAST
Repeati590 – 63041PFTB 3Add
BLAST
Repeati639 – 68042PFTB 4Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 3 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8WQD0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWRLKIADGG NNPYLYSTNN FIGRQTWEFD PNYGTPEERD EVEQARLHFW
60 70 80 90 100
NHRHEIKPSG DTLWRMQFIR EKKFKQTIPQ VKIEDDEEIS YDKVTATMRR
110 120 130 140 150
SVHLLEALLA DDGHWPAENS GPSFFIQPLV MCLYITGHLN SVFPAEHRKE
160 170 180 190 200
ILRYVYSHQN KDGGWGLHME GHSIMFGTTL SYICMRLLGE GPDGGLNGAC
210 220 230 240 250
TRARKWILDH GGAIANPSWG KVWLSILGVH EWVGCNPLPP EFWLFPSFLP
260 270 280 290 300
MSPGKMWSYC RLVFMPMSYL YGRRFVGPIT PLVLQLRKEL YAQPYNDIKW
310 320 330 340 350
KSSRHVCAKE DIYYPHPLLQ DLMWDSLYIL TEPLLTRWPF NKLRKKALAT
360 370 380 390 400
TMRHIHYEDE NSRYITIGSV EKILCMLACW DEDPNGVCFK KHLARIPDYI
410 420 430 440 450
WVAEDGMKMQ TFGSQVWDAS IGIQALLATE LTHDIAPILK KGHEFIKASQ
460 470 480 490 500
VRDNPSGDFK SMYRHISKGS WTFSDQDHGW QLSDCTTIGL TCCLLLSTMP
510 520 530 540 550
PETVGEKMDP EQLKDAVNVI LSLQSENGGL AAWEPAGSSN WLEMLNPIEF
560 570 580 590 600
IEDIVIEHEY VECTGTGMEA LVLFKKLYPK HRTKEVESFL TNAARYLDNT
610 620 630 640 650
QMPDGSWYGE WGICFTYGTY YALGGLAAIE KTYENCQSIR KAVRFLLKTQ
660 670 680 690 700
GEDGGWGESY RSCAEKIYIP LDGNRSTVVH TAWAMLGLMH SKQEERDPIP
710 720 730 740 750
LHRAAKLLIN SQMENGDFPQ QDTTGAFKKN CLLHYPMYRN IYTLWALAQY
760
RKKVLRQPTG I
Length:761
Mass (Da):87,565
Last modified:October 19, 2011 - v1
Checksum:iA8404790E57BBC90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB609123 mRNA. Translation: BAK52535.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB609123 mRNA. Translation: BAK52535.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 3 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus."
    Sawai S., Uchiyama H., Mizuno S., Aoki T., Akashi T., Ayabe S., Takahashi T.
    FEBS Lett. 585:1031-1036(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSHS1_ASTTA
AccessioniPrimary (citable) accession number: F8WQD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 19, 2011
Last modified: October 1, 2014
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.