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F8WQD0 (SHS1_ASTTA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 8. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Shionone synthase

Short name=AtaSHS
EC=5.4.99.46
Gene names
Name:SHS1
Synonyms:ZW1
OrganismAster tataricus (Tartarian aster)
Taxonomic identifier588669 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeAstereaeAustralasian lineagesAster

Protein attributes

Sequence length761 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oxidosqualene cyclase involved in the production of shionone, a triterpenoid with a rare 6/6/6/6 tetracyclic skeleton and a C-3 carbonyl carbon. Produces also small amounts of beta-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone. Ref.1

Catalytic activity

(3S)-2,3-epoxy-2,3-dihydrosqualene = shionone. Ref.1

Tissue specificity

Expressed in stems, leaves and flowers. Lower expression in roots, stolons, rhizomes and radical leaves. Ref.1

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 4 PFTB repeats.

Ontologies

Keywords
   DomainRepeat
   Molecular functionIsomerase
Gene Ontology (GO)
   Biological_processtriterpenoid biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionoxidosqualene cyclase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 761761Shionone synthase
PRO_0000418917

Regions

Repeat148 – 18942PFTB 1
Repeat513 – 55543PFTB 2
Repeat590 – 63041PFTB 3
Repeat639 – 68042PFTB 4

Sequences

Sequence LengthMass (Da)Tools
F8WQD0 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: A8404790E57BBC90

FASTA76187,565
        10         20         30         40         50         60 
MWRLKIADGG NNPYLYSTNN FIGRQTWEFD PNYGTPEERD EVEQARLHFW NHRHEIKPSG 

        70         80         90        100        110        120 
DTLWRMQFIR EKKFKQTIPQ VKIEDDEEIS YDKVTATMRR SVHLLEALLA DDGHWPAENS 

       130        140        150        160        170        180 
GPSFFIQPLV MCLYITGHLN SVFPAEHRKE ILRYVYSHQN KDGGWGLHME GHSIMFGTTL 

       190        200        210        220        230        240 
SYICMRLLGE GPDGGLNGAC TRARKWILDH GGAIANPSWG KVWLSILGVH EWVGCNPLPP 

       250        260        270        280        290        300 
EFWLFPSFLP MSPGKMWSYC RLVFMPMSYL YGRRFVGPIT PLVLQLRKEL YAQPYNDIKW 

       310        320        330        340        350        360 
KSSRHVCAKE DIYYPHPLLQ DLMWDSLYIL TEPLLTRWPF NKLRKKALAT TMRHIHYEDE 

       370        380        390        400        410        420 
NSRYITIGSV EKILCMLACW DEDPNGVCFK KHLARIPDYI WVAEDGMKMQ TFGSQVWDAS 

       430        440        450        460        470        480 
IGIQALLATE LTHDIAPILK KGHEFIKASQ VRDNPSGDFK SMYRHISKGS WTFSDQDHGW 

       490        500        510        520        530        540 
QLSDCTTIGL TCCLLLSTMP PETVGEKMDP EQLKDAVNVI LSLQSENGGL AAWEPAGSSN 

       550        560        570        580        590        600 
WLEMLNPIEF IEDIVIEHEY VECTGTGMEA LVLFKKLYPK HRTKEVESFL TNAARYLDNT 

       610        620        630        640        650        660 
QMPDGSWYGE WGICFTYGTY YALGGLAAIE KTYENCQSIR KAVRFLLKTQ GEDGGWGESY 

       670        680        690        700        710        720 
RSCAEKIYIP LDGNRSTVVH TAWAMLGLMH SKQEERDPIP LHRAAKLLIN SQMENGDFPQ 

       730        740        750        760 
QDTTGAFKKN CLLHYPMYRN IYTLWALAQY RKKVLRQPTG I 

« Hide

References

[1]"Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus."
Sawai S., Uchiyama H., Mizuno S., Aoki T., Akashi T., Ayabe S., Takahashi T.
FEBS Lett. 585:1031-1036(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB609123 mRNA. Translation: BAK52535.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 3 hits.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01787. squalene_cyclas. 1 hit.
PROSITEPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSHS1_ASTTA
AccessionPrimary (citable) accession number: F8WQD0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 19, 2011
Last modified: April 16, 2014
This is version 8 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families