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Protein

7-deoxyloganetin glucosyltransferase

Gene

UGT85A23

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Iridoid glucosyltransferase acting exclusively on 7-deoxyloganetin. No activity with 7-deoxyloganetic acid.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + 7-deoxyloganetin = UDP + 7-deoxyloganin.1 Publication

Kineticsi

kcat is 0.0355 sec(-1) for 7-deoxyloganetin. kcat is 0.0320 sec(-1) for UPD-glucose.

  1. KM=0.202 mM for 7-deoxyloganetin1 Publication
  2. KM=0.117 mM for UPD-glucose1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei307 – 3071UDP-glucoseBy similarity

    GO - Molecular functioni

    • UDP-glucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    • (-)-secologanin biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.324. 1211.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    7-deoxyloganetin glucosyltransferase (EC:2.4.1.324)
    Alternative name(s):
    Iridoid 1-O-glucosyltransferase
    UDP-glucose glucosyltransferase 6
    Short name:
    CrUGT6
    UDP-glycosyltransferase 85A23
    Gene namesi
    Name:UGT85A23
    Synonyms:UGT6
    OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
    Taxonomic identifieri4058 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4874877-deoxyloganetin glucosyltransferasePRO_0000430133Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots.1 Publication

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni364 – 3663UDP-glucose bindingBy similarity
    Regioni381 – 3899UDP-glucose bindingBy similarity
    Regioni403 – 4064UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F8WLS6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSLSSSDYS KKPHAVCIPY PAQGHINPML KLAKLLHYKG FHITFVNTEF
    60 70 80 90 100
    NHKRLLKSRG SDSLKGLHSF QFKTIPDGLP PSDVDATQDI PSLCESTTTH
    110 120 130 140 150
    CLVPFKQLLQ KLNDTSSSEV PPVSCVVSDA VMSFTISAAQ ELDIPEVLFW
    160 170 180 190 200
    TPSACGVLGY MHYAQLIDKG LTPLKDASYF SNGFLDQVLD WIPGMEGIRL
    210 220 230 240 250
    RDLPTFLRTT NPDEYMIKFI LQETERSKKA SAIVLNTFQE LESEVIDSLS
    260 270 280 290 300
    TLLPPIYPIG PLQILQNQVD DESLKVLGSN LWKEEPECLE WLDTKDPNSV
    310 320 330 340 350
    VYVNFGSITV MTNDQLIEFA WGLANSKQNF LWIIRPDLIS GESSILGEEF
    360 370 380 390 400
    VEETKERGLI ASWCHQEQVI NHPAIGGFLT HNGWNSTIES ISSGVPMICW
    410 420 430 440 450
    PFFAEQQTNC RFCCNKWGIG MEINSDVKRD EVESLVKELM VGEKGKEMKK
    460 470 480
    KALEWKNIAE VTTTKPDGSS YSNLEKLIKV LKSKPSH
    Length:487
    Mass (Da):54,611
    Last modified:October 19, 2011 - v1
    Checksum:iA6E423A362B9E0CF
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB591741 mRNA. Translation: BAK55749.1.

    Genome annotation databases

    KEGGiag:BAK55749.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB591741 mRNA. Translation: BAK55749.1.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:BAK55749.

    Enzyme and pathway databases

    BRENDAi2.4.1.324. 1211.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiUGT6_CATRO
    AccessioniPrimary (citable) accession number: F8WLS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 3, 2014
    Last sequence update: October 19, 2011
    Last modified: September 7, 2016
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.