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Protein

12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor

Gene

Gpr31

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-444209. Free fatty acid receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
Short name:
12-(S)-HETE receptor
Short name:
12-HETER
Alternative name(s):
G-protein coupled receptor 31
Gene namesi
Name:Gpr31
Synonyms:Gpr31b, Gpr31c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1354372. Gpr31b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616ExtracellularSequence analysisAdd
BLAST
Transmembranei17 – 3721Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini38 – 5215CytoplasmicSequence analysisAdd
BLAST
Transmembranei53 – 7321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini74 – 9118ExtracellularSequence analysisAdd
BLAST
Transmembranei92 – 11019Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini111 – 13121CytoplasmicSequence analysisAdd
BLAST
Transmembranei132 – 15221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini153 – 18028ExtracellularSequence analysisAdd
BLAST
Transmembranei181 – 20121Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini202 – 21918CytoplasmicSequence analysisAdd
BLAST
Transmembranei220 – 24021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini241 – 26525ExtracellularSequence analysisAdd
BLAST
Transmembranei266 – 28419Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini285 – 31935CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi98.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31931912-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptorPRO_0000415340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiF8VQN3.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089237.

Structurei

3D structure databases

ProteinModelPortaliF8VQN3.
SMRiF8VQN3. Positions 7-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE56. Eukaryota.
ENOG410ZU85. LUCA.
GeneTreeiENSGT00510000046798.
HOGENOMiHOG000285939.
HOVERGENiHBG100615.
InParanoidiF8VQN3.
KOiK08406.
OMAiMERTNCS.
OrthoDBiEOG091G0G4Y.
PhylomeDBiF8VQN3.
TreeFamiTF337237.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8VQN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTNCSAAS TVVETAVGTM LTLECVLGLM GNAVALWTFF YRLKVWKPYA
60 70 80 90 100
VYLFNLVVAD LLLATSLPFF AAFYLKGKTW KLGHMPCQVL LFLLAFSRGV
110 120 130 140 150
GVAFLTTVAL DRYLRVVHPR LRVNLLSLRA AWGISSLIWL LMVVLTPQNL
160 170 180 190 200
LTCRTTQNST ECPSFYPTGG AKAIATCQEV LFFLQVLLPF GLISFCNSGL
210 220 230 240 250
IRTLQKRLRE SDKQPRIRRA RVLVAIVLLL FGLCFLPSVL TRVLVHIFQE
260 270 280 290 300
FKSCSVQQAI VRASDIAGSL TCLHSTLSPA IYCFSNPAFT HSYRKVLKSL
310
RGRRKAAESP SDNLRDSYS
Length:319
Mass (Da):35,642
Last modified:September 21, 2011 - v1
Checksum:i562F575FED1DCBA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31R → H in AAF26668 (PubMed:10501965).Curated
Sequence conflicti67 – 671L → V in AAF26668 (PubMed:10501965).Curated
Sequence conflicti89 – 891V → L in AAF26668 (PubMed:10501965).Curated
Sequence conflicti98 – 981R → C in AAF26668 (PubMed:10501965).Curated
Sequence conflicti106 – 1061T → M in AAF26668 (PubMed:10501965).Curated
Sequence conflicti115 – 1151R → H in AAF26668 (PubMed:10501965).Curated
Sequence conflicti171 – 1711A → T in AAF26668 (PubMed:10501965).Curated
Sequence conflicti209 – 2091R → S in AAF26668 (PubMed:10501965).Curated
Sequence conflicti216 – 2161R → T in AAF26668 (PubMed:10501965).Curated
Sequence conflicti227 – 2271V → M in AAF26668 (PubMed:10501965).Curated
Sequence conflicti261 – 2611V → M in AAF26668 (PubMed:10501965).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140708 Genomic DNA. Translation: AAF26668.1.
AC166360 Genomic DNA. No translation available.
CCDSiCCDS37438.1.
RefSeqiNP_001013854.2. NM_001013832.2.
UniGeneiMm.335670.
Mm.390936.

Genome annotation databases

EnsembliENSMUST00000091648; ENSMUSP00000089237; ENSMUSG00000071311.
GeneIDi436440.
KEGGimmu:436440.
UCSCiuc008alx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140708 Genomic DNA. Translation: AAF26668.1.
AC166360 Genomic DNA. No translation available.
CCDSiCCDS37438.1.
RefSeqiNP_001013854.2. NM_001013832.2.
UniGeneiMm.335670.
Mm.390936.

3D structure databases

ProteinModelPortaliF8VQN3.
SMRiF8VQN3. Positions 7-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000089237.

Chemistry

GuidetoPHARMACOLOGYi98.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiF8VQN3.

Protocols and materials databases

DNASUi436440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000091648; ENSMUSP00000089237; ENSMUSG00000071311.
GeneIDi436440.
KEGGimmu:436440.
UCSCiuc008alx.1. mouse.

Organism-specific databases

CTDi436440.
MGIiMGI:1354372. Gpr31b.

Phylogenomic databases

eggNOGiENOG410IE56. Eukaryota.
ENOG410ZU85. LUCA.
GeneTreeiENSGT00510000046798.
HOGENOMiHOG000285939.
HOVERGENiHBG100615.
InParanoidiF8VQN3.
KOiK08406.
OMAiMERTNCS.
OrthoDBiEOG091G0G4Y.
PhylomeDBiF8VQN3.
TreeFamiTF337237.

Enzyme and pathway databases

ReactomeiR-MMU-444209. Free fatty acid receptors.

Miscellaneous databases

PROiF8VQN3.
SOURCEiSearch...

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPR31_MOUSE
AccessioniPrimary (citable) accession number: F8VQN3
Secondary accession number(s): Q9JLS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: September 21, 2011
Last modified: September 7, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.