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Protein

Unconventional myosin-X

Gene

Myo10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments (By similarity). Regulates cell shape, cell spreading and cell adhesion. Stimulates the formation and elongation of filopodia. May play a role in neurite outgrowth and axon guidance. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts.By similarity2 Publications
Isoform Headless: Functions as a dominant-negative regulator of isoform 1, suppressing its filopodia-inducing and axon outgrowth-promoting activities. In hippocampal neurons, it increases VASP retention in spine heads to induce spine formation and spine head expansion.

GO - Molecular functioni

GO - Biological processi

  • cell motility Source: MGI
  • cytoskeleton-dependent intracellular transport Source: UniProtKB
  • positive regulation of cell-cell adhesion Source: MGI
  • regulation of cell shape Source: UniProtKB
  • regulation of filopodium assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Transport

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-X
Alternative name(s):
Unconventional myosin-10
Gene namesi
Name:Myo10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:107716. Myo10.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • filopodium Source: MGI
  • filopodium membrane Source: UniProtKB-SubCell
  • filopodium tip Source: UniProtKB
  • lamellipodium Source: MGI
  • myosin complex Source: UniProtKB-KW
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
  • nucleolus Source: MGI
  • plasma membrane Source: MGI
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004162441 – 2062Unconventional myosin-XAdd BLAST2062

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei961PhosphoserineBy similarity1
Modified residuei964PhosphoserineBy similarity1
Modified residuei967PhosphoserineBy similarity1
Modified residuei1162PhosphothreonineBy similarity1

Post-translational modificationi

The initiator methionine for isoform Headless is removed.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiF8VQB6.
MaxQBiF8VQB6.
PaxDbiF8VQB6.
PeptideAtlasiF8VQB6.
PRIDEiF8VQB6.

PTM databases

iPTMnetiF8VQB6.
PhosphoSitePlusiF8VQB6.

Expressioni

Tissue specificityi

Detected in brain, heart, kidney, liver, stomach, skeletal muscle, lung, testis and skin. Isoform Headless is expressed in embryonic and neuronal stem cells, and enriched in proliferating and migrating cells.2 Publications

Gene expression databases

BgeeiENSMUSG00000022272.
ExpressionAtlasiF8VQB6. baseline and differential.
GenevisibleiF8VQB6. MM.

Interactioni

Subunit structurei

Monomer, when in an inactive confomation in the cytosol. Homodimer in its active, membrane-bound conformation; antiparallel coiled coil-mediated dimer formation. Interacts with ECM29. Interacts with DCC and ITGB5; the presence of DCC inhibits ITGB5 binding. Interacts with tubulin; ITGB5 or DCC binding inhibits tubulin binding. Interacts strongly with CALM3 and weakly with CALM, the CALM3 interaction is essential for function in filopodial extension and motility. Interacts with ITGB1, ITGB3 and ITGB5 (By similarity). Interacts with NEO1. Interacts with VASP.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Neo1P977983EBI-6445959,EBI-774991

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201661. 1 interactor.
IntActiF8VQB6. 4 interactors.
STRINGi10090.ENSMUSP00000106087.

Structurei

3D structure databases

ProteinModelPortaliF8VQB6.
SMRiF8VQB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 739Myosin motorAdd BLAST677
Domaini742 – 771IQ 1PROSITE-ProRule annotationAdd BLAST30
Domaini765 – 794IQ 2PROSITE-ProRule annotationAdd BLAST30
Domaini788 – 817IQ 3PROSITE-ProRule annotationAdd BLAST30
Domaini1216 – 1314PH 1PROSITE-ProRule annotationAdd BLAST99
Domaini1396 – 1501PH 2PROSITE-ProRule annotationAdd BLAST106
Domaini1551 – 1699MyTH4PROSITE-ProRule annotationAdd BLAST149
Domaini1704 – 2048FERMPROSITE-ProRule annotationAdd BLAST345

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni815 – 907SAHBy similarity1 PublicationAdd BLAST93
Regioni882 – 932Mediates antiparallel dimerizationBy similarityAdd BLAST51

Domaini

Interaction between the motor domain and the tail leads to an inactive, monomeric conformation. Phospholipid binding via the PH domains leads to the formation of the active, dimeric form of the protein and strongly increases actin-dependent ATPase activity and motor activity (By similarity).By similarity
Interacts with membranes containing phosphatidylinositol-3,4,5-trisphosphate via the PH domains.By similarity
IQ 3 domain mediates high-affinity calcium-dependent binding to CALM3/CLP.By similarity
The SAH (single alpha-helix) region is characterized by a high content of charged residues which are predicted to stabilize the alpha-helical structure by ionic bonds. It can refold after extension suggesting an in vivo force-dependent function. The isolated SAH domain is monomeric; however, in its distal part seems to form a semirigid helical structure which overlaps with a region shown to mediate antiparallel coiled coil-mediated dimerization.Curated

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 3 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 MyTH4 domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000007044.
InParanoidiF8VQB6.
KOiK12559.
OMAiPEDASQW.
OrthoDBiEOG091G006V.
TreeFamiTF316834.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 5 hits.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR031971. MYO10_CC.
IPR001609. Myosin_head_motor_dom.
IPR000857. MyTH4_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00612. IQ. 3 hits.
PF16735. MYO10_CC. 1 hit.
PF00063. Myosin_head. 1 hit.
PF00784. MyTH4. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00295. B41. 1 hit.
SM00015. IQ. 3 hits.
SM00242. MYSc. 1 hit.
SM00139. MyTH4. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 2 hits.
SSF50729. SSF50729. 4 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50057. FERM_3. 1 hit.
PS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51016. MYTH4. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: F8VQB6-1) [UniParc]FASTAAdd to basket
Also known as: 240kDa, FL-myo10

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSFFPEGAR VWLRENGQHF PSTVNSCAEG VVVFQTDYGQ VFTYKQSTIT
60 70 80 90 100
NQKVTAMHPL HEEGVDDMAS LAELHGGSIM YNLFQRYKRN QIYTYIGSII
110 120 130 140 150
ASVNPYQPIA GLYERATMEE YSRCHLGELP PHIFAIANEC YRCLWKRHDN
160 170 180 190 200
QCVLISGESG AGKTESTKLI LKFLSVISQQ TLDLGLQEKT SSVEQAILQS
210 220 230 240 250
SPIMEAFGNA KTVYNNNSSR FGKFVQLNIC QQGNIQGGRI VDYLLEKNRV
260 270 280 290 300
VRQNPGERNY HIFYALLAGL DQGEREEFYL SLPENYHYLN QSGCTEDKTI
310 320 330 340 350
SDQESFRQVI TAMEVMQFSK EEVREVLRLL AGILHLGNIE FITAGGAQIP
360 370 380 390 400
FKTALGRSAD LLGLDPTQLT DALTQRSMIL RGEEILTPLS VQQAVDSRDS
410 420 430 440 450
LAMALYARCF EWVIKKINSR IKGKDDFKSI GILDIFGFEN FEVNHFEQFN
460 470 480 490 500
INYANEKLQE YFNKHIFSLE QLEYSREGLV WEDIDWIDNG ECLDLIEKKL
510 520 530 540 550
GLLALINEES HFPQATDSTL LEKLHSQHAN NHFYVKPRVA VNNFGVKHYA
560 570 580 590 600
GEVQYDVRGI LEKNRDTFRD DLLNLLRESR FDFIYDLFEH VSSRNNQDTL
610 620 630 640 650
KCGSKHRRPT VSSQFKDSLH SLMATLSSSN PFFVRCIKPN TQKMPDQFDQ
660 670 680 690 700
VVVLNQLRYS GMLETVRIRK AGYAVRRPFQ DFYKRYKVLM RNLALPDDIR
710 720 730 740 750
GKCTVLLQVY DASNSEWQLG KTKVFLRESL EQKLEKRREE EIDRAAMVIR
760 770 780 790 800
AHILGYLARK QYRKVLCGVV TIQKNYRAFL ARKKFLHLKK AAIVFQKQLR
810 820 830 840 850
GQLARRVYRQ LLAEKRELEE KKRREEEKKR EEEERERERA QREADLLRAH
860 870 880 890 900
QEAETRRQQE LEALQKSQRE ADLTRELEKQ RENKQVEEIL RLEKEIEDLQ
910 920 930 940 950
RMKERQELSL TEASLQKLQQ LRDEELRRLE DEACRAAQEF LESLNFDEID
960 970 980 990 1000
ECVRNIERSL SVGSEISGEE LSELAESASG EKPNFNFSQP YPAEEEVDEG
1010 1020 1030 1040 1050
FEADDDAFKD SPNPSEHGHS DQRTSGIRTS DDSSEEDPYM NYTVVPTSPS
1060 1070 1080 1090 1100
ADSTVLLAAS MQDSASLHNS SSGESTYCMP QNNGDLPSPD GDYDYDQDDY
1110 1120 1130 1140 1150
EDGAITSGSS VTFSNSYGSQ WSPDYRYSVG TYNSSGAYRF SSEGAQSSFE
1160 1170 1180 1190 1200
DSEEDFDSRF DTDDELSYRR DSVYSCVTLP YFHSFLYMKG GLMNSWKRRW
1210 1220 1230 1240 1250
CVLKDETFLW FRSKQEALKQ GWLHKKGGGS STLSRRNWKK RWFVLRQSKL
1260 1270 1280 1290 1300
MYFENDSEEK LKGTVEVRTA KEIIDNTSKE NGIDIILADR TFHLIAESPE
1310 1320 1330 1340 1350
DASQWFSVLS QVHSSTDQEI REMHDEQANP QNAVGTLDVG LIDSVCASDS
1360 1370 1380 1390 1400
PDRPNSFVII TANRVLHCNA DTPEEMHHWI TLLQRSKGDT RVEGQEFIVR
1410 1420 1430 1440 1450
GWLHKEVKNS PKMSSLKLKK RWFVLTHNSL DYYKSSEKNA LKLGTLVLNS
1460 1470 1480 1490 1500
LCSVVPPDEK IFKETGYWNV TVYGRKHCYR LYTKLLNEAT RWSSAIQNVT
1510 1520 1530 1540 1550
DTKAPIDTPT QQLIQDIKEN CLNSDVVEQI YKRNPILRYT HHPLHSPLLP
1560 1570 1580 1590 1600
LPYGDINLNL LKDKGYTTLQ DEAIKIFNSL QQLESMSDPI PIIQGILQTG
1610 1620 1630 1640 1650
HDLRPLRDEL YCQLIKQTNK VPHPGSVGNL YSWQILTCLS CTFLPSRGIL
1660 1670 1680 1690 1700
KYLKFHLKRI REQFPGTEME KYALFIYESL KKTKCREFVP SRDEIEALIH
1710 1720 1730 1740 1750
RQEMTSTVYC HGGGSCKITI NSHTTAGEVV EKLIRGLAME DSRNMFALFE
1760 1770 1780 1790 1800
YNGQVDKAIE SRTIVADVLA KFEKLAATSE AGDAPWKFYF KLYCFLDTDS
1810 1820 1830 1840 1850
MPKDSVEFAF MFEQAHEAVI HGHHPAPEES LQVLAALRLQ YLQGDYTPHT
1860 1870 1880 1890 1900
SIPPLEEVYS VQRLRARISQ STKTFTPYER LEKRRTSFLE GTLRRSFRTG
1910 1920 1930 1940 1950
SVVRQKAEEE QMLDMWIKEE VCSARTSIID KWKKLQGMNQ EQAMAKYMAL
1960 1970 1980 1990 2000
IKEWPGYGST LFDVECKEGG FPQELWLGVS ADAVSVYKRG EGKPLEVFQY
2010 2020 2030 2040 2050
EHILSFGAPL ANTYKIVVDE RELLFETSEV VDVAKLMKAY ISMIVKKRYS
2060
TTRSVSSQGS SR
Length:2,062
Mass (Da):237,323
Last modified:September 21, 2011 - v1
Checksum:iFD685BEE8B88DE30
GO
Isoform Headless (identifier: F8VQB6-2) [UniParc]FASTAAdd to basket
Also known as: 165kDa, Hdl-myo10

The sequence of this isoform differs from the canonical sequence as follows:
     1-643: Missing.

Note: Produced by alternative promoter usage.
Show »
Length:1,419
Mass (Da):163,803
Checksum:iA4213D2D117A6DFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77 – 78GS → VA in CAB56466 (PubMed:10799329).Curated2
Sequence conflicti253 – 254QN → RI in CAB56466 (PubMed:10799329).Curated2
Sequence conflicti273G → E in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti501G → R in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti870E → A in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti984N → S in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti993A → E in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1032D → E in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1042Y → D in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1083N → P in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1371D → Y in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1602D → H in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1647R → L in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1805S → G in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1939N → T in CAB56466 (PubMed:10799329).Curated1
Sequence conflicti1982D → E in CAB56466 (PubMed:10799329).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0549781 – 643Missing in isoform Headless. CuratedAdd BLAST643

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249706 mRNA. Translation: CAB56466.2.
AC115746 Genomic DNA. No translation available.
AC131178 Genomic DNA. No translation available.
BC024692 mRNA. Translation: AAH24692.1.
CCDSiCCDS37049.1. [F8VQB6-1]
PIRiA59297.
RefSeqiNP_062345.2. NM_019472.2. [F8VQB6-1]
XP_006520088.1. XM_006520025.3. [F8VQB6-2]
UniGeneiMm.60590.

Genome annotation databases

EnsembliENSMUST00000110457; ENSMUSP00000106087; ENSMUSG00000022272. [F8VQB6-1]
GeneIDi17909.
KEGGimmu:17909.
UCSCiuc007vjc.1. mouse. [F8VQB6-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249706 mRNA. Translation: CAB56466.2.
AC115746 Genomic DNA. No translation available.
AC131178 Genomic DNA. No translation available.
BC024692 mRNA. Translation: AAH24692.1.
CCDSiCCDS37049.1. [F8VQB6-1]
PIRiA59297.
RefSeqiNP_062345.2. NM_019472.2. [F8VQB6-1]
XP_006520088.1. XM_006520025.3. [F8VQB6-2]
UniGeneiMm.60590.

3D structure databases

ProteinModelPortaliF8VQB6.
SMRiF8VQB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201661. 1 interactor.
IntActiF8VQB6. 4 interactors.
STRINGi10090.ENSMUSP00000106087.

PTM databases

iPTMnetiF8VQB6.
PhosphoSitePlusiF8VQB6.

Proteomic databases

EPDiF8VQB6.
MaxQBiF8VQB6.
PaxDbiF8VQB6.
PeptideAtlasiF8VQB6.
PRIDEiF8VQB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110457; ENSMUSP00000106087; ENSMUSG00000022272. [F8VQB6-1]
GeneIDi17909.
KEGGimmu:17909.
UCSCiuc007vjc.1. mouse. [F8VQB6-1]

Organism-specific databases

CTDi4651.
MGIiMGI:107716. Myo10.

Phylogenomic databases

eggNOGiKOG4229. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000007044.
InParanoidiF8VQB6.
KOiK12559.
OMAiPEDASQW.
OrthoDBiEOG091G006V.
TreeFamiTF316834.

Miscellaneous databases

PROiF8VQB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022272.
ExpressionAtlasiF8VQB6. baseline and differential.
GenevisibleiF8VQB6. MM.

Family and domain databases

Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 5 hits.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR031971. MYO10_CC.
IPR001609. Myosin_head_motor_dom.
IPR000857. MyTH4_dom.
IPR027417. P-loop_NTPase.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00373. FERM_M. 1 hit.
PF00612. IQ. 3 hits.
PF16735. MYO10_CC. 1 hit.
PF00063. Myosin_head. 1 hit.
PF00784. MyTH4. 1 hit.
PF00169. PH. 2 hits.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00295. B41. 1 hit.
SM00015. IQ. 3 hits.
SM00242. MYSc. 1 hit.
SM00139. MyTH4. 1 hit.
SM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 2 hits.
SSF50729. SSF50729. 4 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50057. FERM_3. 1 hit.
PS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51016. MYTH4. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO10_MOUSE
AccessioniPrimary (citable) accession number: F8VQB6
Secondary accession number(s): Q8R3S0, Q9JJY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-10 (MYH10).Curated
Originally predicted to contain a coiled coil domain but shown to contain a stable SAH domain instead.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.