ID   F8VPT3_MOUSE            Unreviewed;      1931 AA.
AC   F8VPT3;
DT   21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT   21-SEP-2011, sequence version 1.
DT   27-MAR-2024, entry version 82.
DE   SubName: Full=Lactase {ECO:0000313|Ensembl:ENSMUSP00000073190.7};
GN   Name=Lct {ECO:0000313|Ensembl:ENSMUSP00000073190.7,
GN   ECO:0000313|MGI:MGI:104576};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000073190.7, ECO:0000313|Proteomes:UP000000589};
RN   [1] {ECO:0000313|Ensembl:ENSMUSP00000073190.7, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000073190.7,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2] {ECO:0000313|Ensembl:ENSMUSP00000073190.7}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000073190.7};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
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DR   RefSeq; NP_001074547.1; NM_001081078.2.
DR   AlphaFoldDB; F8VPT3; -.
DR   SMR; F8VPT3; -.
DR   STRING; 10090.ENSMUSP00000073190; -.
DR   iPTMnet; F8VPT3; -.
DR   PhosphoSitePlus; F8VPT3; -.
DR   MaxQB; F8VPT3; -.
DR   PaxDb; 10090-ENSMUSP00000073190; -.
DR   PeptideAtlas; F8VPT3; -.
DR   ProteomicsDB; 316788; -.
DR   Antibodypedia; 2365; 405 antibodies from 16 providers.
DR   Ensembl; ENSMUST00000073490.7; ENSMUSP00000073190.7; ENSMUSG00000026354.9.
DR   GeneID; 226413; -.
DR   KEGG; mmu:226413; -.
DR   UCSC; uc007clm.1; mouse.
DR   AGR; MGI:104576; -.
DR   CTD; 3938; -.
DR   MGI; MGI:104576; Lct.
DR   VEuPathDB; HostDB:ENSMUSG00000026354; -.
DR   eggNOG; KOG0626; Eukaryota.
DR   GeneTree; ENSGT00940000155324; -.
DR   HOGENOM; CLU_001859_5_3_1; -.
DR   InParanoid; F8VPT3; -.
DR   OMA; QITHYKV; -.
DR   OrthoDB; 3373839at2759; -.
DR   PhylomeDB; F8VPT3; -.
DR   TreeFam; TF314803; -.
DR   Reactome; R-MMU-189085; Digestion of dietary carbohydrate.
DR   BioGRID-ORCS; 226413; 3 hits in 76 CRISPR screens.
DR   ChiTaRS; Lct; mouse.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; F8VPT3; Protein.
DR   Bgee; ENSMUSG00000026354; Expressed in small intestine Peyer's patch and 55 other cell types or tissues.
DR   GO; GO:0005903; C:brush border; ISO:MGI.
DR   GO; GO:0098591; C:external side of apical plasma membrane; ISO:MGI.
DR   GO; GO:0008422; F:beta-glucosidase activity; ISO:MGI.
DR   GO; GO:0004336; F:galactosylceramidase activity; ISO:MGI.
DR   GO; GO:0004348; F:glucosylceramidase activity; ISO:MGI.
DR   GO; GO:0000016; F:lactase activity; ISO:MGI.
DR   GO; GO:0140749; F:phlorizin hydrolase activity; ISO:MGI.
DR   GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR   GO; GO:0016740; F:transferase activity; ISO:MGI.
DR   GO; GO:2000892; P:cellobiose catabolic process; ISO:MGI.
DR   GO; GO:0046477; P:glycosylceramide catabolic process; ISO:MGI.
DR   GO; GO:0005990; P:lactose catabolic process; ISO:MGI.
DR   GO; GO:1901733; P:quercetin catabolic process; ISO:MGI.
DR   GO; GO:0045471; P:response to ethanol; ISO:MGI.
DR   GO; GO:0001666; P:response to hypoxia; ISO:MGI.
DR   GO; GO:0010040; P:response to iron(II) ion; ISO:MGI.
DR   GO; GO:0010288; P:response to lead ion; ISO:MGI.
DR   GO; GO:0010045; P:response to nickel cation; ISO:MGI.
DR   GO; GO:0007584; P:response to nutrient; ISO:MGI.
DR   GO; GO:0010033; P:response to organic substance; ISO:MGI.
DR   GO; GO:0009410; P:response to xenobiotic stimulus; ISO:MGI.
DR   Gene3D; 3.20.20.80; Glycosidases; 4.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR018120; Glyco_hydro_1_AS.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR   PANTHER; PTHR10353:SF36; KLOTHO (MAMMALIAN AGING-ASSOCIATED PROTEIN) HOMOLOG; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 4.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 4.
DR   PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 3.
DR   Genevisible; F8VPT3; MM.
PE   1: Evidence at protein level;
KW   Glycosidase {ECO:0000256|RuleBase:RU004468};
KW   Hydrolase {ECO:0000256|RuleBase:RU004468};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|MaxQB:F8VPT3,
KW   ECO:0007829|PeptideAtlas:F8VPT3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..1931
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003379233"
FT   TRANSMEM        1887..1907
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   ACT_SITE        1753
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ   SEQUENCE   1931 AA;  217802 MW;  162821F8CCBED384 CRC64;
     MELPWTALFL STFLLGLSCQ GNDWESDRNF ISAAGPLTND LVHNLNHPLG KQGSDLVAGD
     TDIYVCQQPL PSFLPQYFSS LRASQVTHYK VLLSWAQLLP KGSSKNPDQE AVQCYRQLLQ
     SLKDAQLQPM VVLFHQMPPT STIQRDGGFA DLFADYATLA FQSFGDLVEI WFTFSDLEKV
     IMGLPHQHLK ASGLQTLSDA HRKAFDVYHR KYSSQGGKLS VVLKAEDLPK LLPDPSSAAL
     VKGSVDFLSL DLSYDCQSIS TLPQKLSELQ NLEPKVKVFV YTLKPQDCPS TGMSPASLLF
     RLLEAINKDQ VQTVGFDVSA FLSCTSSSEE SPSCSLTYSS LALQSEQQHE TVVSSFPGSA
     YQRVWAALAN QSREERDAFL QDVFPEGFLW GVSTGAFNVE GGWAEGGRGP SIWDHYSNLN
     AAESQATAKV ASDSYHKPVS DVALLRGLRA DVYKFSISWS RIFPFGQRTS PNLQGVAYYN
     KLIDSLLDSQ VEPMATLFHW DLPQALQEQG GWQNESVVDA FLDYAAFCFS TFGDRVKLWV
     TFHEPWVISY AGYGTGQHAP AISDPGVASF KVAHLILKAH ARTWHHYNYH HRQKQQGRVG
     IVLNSDWAEP LDGKSPQDLA AAERYLHFML GWFAHPIFID GDYPAALRAQ IQHTNQQCGR
     PLAQLPEFTE AEKRLLKGSA DFLGLSHYTS RLISKAGQQT CIPSYDNIGG FSQHVDPKWP
     QTASPWIRVV PWGIRRLLRF ASLEYTKGKL PIFLAGNGMP IGEGSDLFDD SMRVNYLNLY
     INEVLKAVKE DSVDVRSYIA RSLIDGYEGP LGYSQRFGLY HVNFNDSSRP RTPRKSAYFF
     TSIIEKNGFP AKKVKRNPLP LKADFSSRAR AAFSFPSEVP SKAKVVWEKF SRQPKFERDL
     FYHGTFRDDF LWGVSSSAYQ IEGGWDADGK GPSIWDNFTH TPGNGVKDNA TGDIACDSYH
     QLDADINILR TLKVKSYRFS ISWPRIFPTG RNSSINKQGV DYYNKLIDRL LESNIFPMVT
     LFHWDLPQAL QDIGGWENPS LIELFDSYAD FCFKTFGDRV KFWMTFNEPW CSAVLGYSSG
     IFPPNVQDPG SLSYKVSHVI IKAHARVYHT YDEKYRQEQK GVISLSLNTH WVEPKDPGVQ
     RDVEAADRML QFNLGWFAHP IFKNGDYPDV MKWNVGNRSE LQHLASSRLP SFTEEEKNYI
     RGTADVFCIN TYTSVFAQHV TPRLNPPSYD NDMELKASDM NSSALISMMH QDVPWGMRRL
     LNWIKEEYGN IPIYITENGQ GLTNPTLDDT ERIFYHKTYI NEALKAYRLD GVDLRGYSAW
     ALMDNFEWLH GYTMRFGLYH VDFDHVNRPR TARASARYYT EVITNNGMPL AKEDEFLYGE
     FPKGFIWSAA SASYQVEGAW RADGKGLSIW DTFSHTPLKI GNDDNGDVAC DSYHKIAEDV
     VALQNLGVSH YRFSISWPRI LPDGTTKFIN EAGLNYYVRF IDALLAAGIT PQVTMYHWDL
     PQALQDVGGW ENETVVQRFK DYADVLFRRL GDKVKFWITL NEPFVIAAHG YGSGVSAPGI
     SFRPGTAPYT AGHNLIKAHA EAWHLYNSTY RNSQGGVISI TISSDWAEPR DPSNQEDVEA
     ARRYVQFMGG WFAHPIFKNG DYPEVMKTRI RDRSLAAGLN KSRLPEFTES EKKKIQGTFD
     FFGFNHYTTV LAYNLNYAAA VSSFDADRGV ASITDRSWPD SGSFWLKMTP FGFRRILNWL
     KEEYNNPLIY VTENGVSRRG DPELNDTDRI YYLRSYINEA LKAVRDKVDL RGYTVWSIMD
     NFEWATGFAE RFGVHFVNRS DPSLPRIPKA SAKVYASIVR CNGFPDPAQG PHPCLQQPED
     AGPTASPVKS EVPFLGLMLG TAEAQTALYV LFALVLLGVC SVAFLLYKYC KRSKQGTTQP
     GHHGLSQISS F
//