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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Salmonella bongori (strain ATCC 43975 / DSM 13772 / NCTC 12419)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotationSAAS annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotationSAAS annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi270 – 2701Manganese 2UniRule annotation
Metal bindingi275 – 2751Manganese 1UniRule annotation
Metal bindingi275 – 2751Manganese 2UniRule annotation
Active sitei282 – 2821UniRule annotation
Metal bindingi293 – 2931Manganese 2UniRule annotation
Metal bindingi352 – 3521Manganese 1UniRule annotation
Metal bindingi354 – 3541Manganese 1UniRule annotation
Metal bindingi354 – 3541Manganese 2UniRule annotation
Active sitei356 – 3561UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

AminopeptidaseUniRule annotationSAAS annotation, Hydrolase, Protease

Keywords - Ligandi

ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciSBON218493:GJAH-3946-MONOMER.

Protein family/group databases

MEROPSiM17.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation
Alternative name(s):
Leucine aminopeptidaseUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:SBG_3897Imported
OrganismiSalmonella bongori (strain ATCC 43975 / DSM 13772 / NCTC 12419)Imported
Taxonomic identifieri218493 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000000289 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

KOiK01255.
OMAiHELINAS.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F8VGZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG
60 70 80 90 100
ELEGKPGQTL LLHHVPNVLS ERILLIGCGK ERELDERQYK QVIQKTINTL
110 120 130 140 150
NDTGSMEAVC FLTELHVKGR NNYWKVRQAV ETAKETLYSF DQLKTNKSEP
160 170 180 190 200
RRPLRKMVFN VPTRRELTSG ERAIQHGLAI AAGIKAAKDL GNMPPNICNA
210 220 230 240 250
AYLASQARQL ADSYSTNVIT RVIGEQQMRE LGMNAYLAVG HGSQNESLMS
260 270 280 290 300
VIEYKGNSSE EARPIVLVGK GLTFDSGGIS IKPSEGMDEM KYDMCGAAAV
310 320 330 340 350
YGVMRMVAEL QLPINVIGVL AGCENMPGGR AYRPGDVLTT MSGQTVEVLN
360 370 380 390 400
TDAEGRLVLC DVLTYVERFE PEAVIDVATL TGACVIALGH HITGLMSNHN
410 420 430 440 450
PLAHELIGAS EQAGDRAWRL PLGDEYQEQL ESNFADMANI GGRPGGAITA
460 470 480 490 500
GCFLSRFTRK YNWAHLDIAG TAWRSGKAKG ATGRPVALLA QFLLNRAGFN

GEE
Length:503
Mass (Da):54,867
Last modified:September 20, 2011 - v1
Checksum:i4049C24FDC899C51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR877557 Genomic DNA. Translation: CCC32939.1.
RefSeqiWP_000397160.1. NC_015761.1.
YP_004732681.1. NC_015761.1.

Genome annotation databases

EnsemblBacteriaiCCC32939; CCC32939; SBG_3897.
GeneIDi10969183.
KEGGisbg:SBG_3897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR877557 Genomic DNA. Translation: CCC32939.1.
RefSeqiWP_000397160.1. NC_015761.1.
YP_004732681.1. NC_015761.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM17.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCC32939; CCC32939; SBG_3897.
GeneIDi10969183.
KEGGisbg:SBG_3897.

Phylogenomic databases

KOiK01255.
OMAiHELINAS.

Enzyme and pathway databases

BioCyciSBON218493:GJAH-3946-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43975 / DSM 13772 / NCTC 12419Imported.

Entry informationi

Entry nameiF8VGZ6_SALBC
AccessioniPrimary (citable) accession number: F8VGZ6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 20, 2011
Last sequence update: September 20, 2011
Last modified: March 31, 2015
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.