Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

F8UU09

- F8UU09_9INFA

UniProt

F8UU09 - F8UU09_9INFA

Protein
Submitted name:

Neuraminidase

Gene

NA

Organism
Influenza A virus (A/Netherlands/2631_1202/2010(H1N1))
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (21 Sep 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.SAAS annotation

    Cofactori

    Binds 1 calcium ion per subunit.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei150 – 1501Phosphate 1
    Binding sitei199 – 1991Phosphate 1; via amide nitrogen
    Binding sitei293 – 2931Phosphate 2
    Metal bindingi294 – 2941Calcium; via carbonyl oxygenImported
    Metal bindingi298 – 2981Calcium; via carbonyl oxygenImported
    Metal bindingi324 – 3241CalciumImported
    Metal bindingi342 – 3421Calcium; via carbonyl oxygenImported
    Metal bindingi344 – 3441Calcium; via carbonyl oxygenImported
    Binding sitei368 – 3681Phosphate 2
    Binding sitei402 – 4021Phosphate 2
    Binding sitei455 – 4551Phosphate 1

    GO - Molecular functioni

    1. exo-alpha-sialidase activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseSAAS annotation, Hydrolase

    Keywords - Ligandi

    CalciumSAAS annotationImported, Metal-bindingSAAS annotationImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    NeuraminidaseImported
    Gene namesi
    Name:NAImported
    OrganismiInfluenza A virus (A/Netherlands/2631_1202/2010(H1N1))Imported
    Taxonomic identifieri1027873 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

    Subcellular locationi

    Virion membrane SAAS annotation. Host apical cell membrane SAAS annotation; Single-pass type II membrane protein SAAS annotation

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membraneSAAS annotation, Host membrane, Membrane, VirionSAAS annotation

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881N-linked (GlcNAc...)Imported
    Glycosylationi146 – 1461N-linked (GlcNAc...)Imported
    Glycosylationi235 – 2351N-linked (GlcNAc...)Imported

    Keywords - PTMi

    Disulfide bondSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.SAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4B7JX-ray2.42A1-469[»]
    4B7MX-ray2.50A/B1-469[»]
    4B7NX-ray2.84A1-469[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helixSAAS annotation

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR001860. Glyco_hydro_34.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF00064. Neur. 1 hit.
    [Graphical view]
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F8UU09-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPNQKIITI GSVCMTIGMA NLILQIGNII SIWISHSIQL GNQNQIETCN    50
    QSVITYENNT WVNQTYVNIS NTNFAAGQSV VSVKLAGNSS LCPVSGWAIY 100
    SKDNSIRIGS KGDVFVIREP FISCSPLECR TFFLTQGALL NDKHSNGTIK 150
    DRSPYRTLMS CPIGEVPSPY NSRFESVAWS ASACHDGINW LTIGISGPDN 200
    GAVAVLKYNG IITDTIKSWR NNRLRTQESE CACVNGSCFT VMTDGPSDGQ 250
    ASYKIFRIEK GKIVKSVEMN APNYHYEECS CYPDSSEITC VCRDNWHGSN 300
    RPWVSFNQNL EYQIGYICSG IFGDNPRPND KTGSCGPVSS NGANGVKGFS 350
    FKYGNGVWIG RTKSISSRNG FEMIWDPNGW TGTDNDFSIK QDIVGINEWS 400
    GYSGSFVQHP ELTGLDCIRP CFWVELIRGR PKENTIWTSG SSISFCGVNS 450
    DTVGWSWPDG AELPFTIDK 469
    Length:469
    Mass (Da):51,656
    Last modified:September 21, 2011 - v1
    Checksum:i48F0F60CD8119D39
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF906185 Viral cRNA. Translation: AEE69001.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JF906185 Viral cRNA. Translation: AEE69001.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4B7J X-ray 2.42 A 1-469 [» ]
    4B7M X-ray 2.50 A/B 1-469 [» ]
    4B7N X-ray 2.84 A 1-469 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR001860. Glyco_hydro_34.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF00064. Neur. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Multidrug Resistant 2009 A/H1N1 Influenza Clinical Isolate with a Neuraminidase I223R Mutation Retains Its Virulence and Transmissibility in Ferrets."
      van der Vries E., Veldhuis Kroeze E.J., Stittelaar K.J., Linster M., Van der Linden A., Schrauwen E.J., Leijten L.M., van Amerongen G., Schutten M., Kuiken T., Osterhaus A.D., Fouchier R.A., Boucher C.A., Herfst S.
      PLoS Pathog. 7:E1002276-E1002276(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A/Netherlands/2631_1202/2010Imported.
    2. "H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis."
      van der Vries E., Collins P.J., Vachieri S.G., Xiong X., Liu J., Walker P.A., Haire L.F., Hay A.J., Schutten M., Osterhaus A.D., Martin S.R., Boucher C.A., Skehel J.J., Gamblin S.J.
      PLoS Pathog. 8:e1002914-e1002914(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) IN COMPLEX WITH CALCIUM, GLYCOSYLATION AT ASN-88; ASN-146 AND ASN-235.

    Entry informationi

    Entry nameiF8UU09_9INFA
    AccessioniPrimary (citable) accession number: F8UU09
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3