F8UU09 (F8UU09_9INFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Neuraminidase EMBL AEE69001.1

Gene names

Name:

NA EMBL AEE69001.1

Organism

Influenza A virus (A/Netherlands/2631_1202/2010(H1N1)) EMBL AEE69001.1

Taxonomic identifier

1027873 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. SAAS SAAS011040
Subunit structure	Homotetramer By similarity. SAAS SAAS011040
Subcellular location	Virion membrane. Host apical cell membrane; Single-pass type II membrane protein By similarity SAAS SAAS011040.

Ontologies

Keywords
Cellular component	Host cell membrane SAAS SAAS011040 Host membrane Membrane Virion SAAS SAAS011040
Domain	Transmembrane Transmembrane helix SAAS SAAS011040
Ligand	Calcium PDB 4B7J PDB 4B7M PDB 4B7N Metal-binding PDB 4B7J PDB 4B7M PDB 4B7N
Molecular function	Glycosidase SAAS SAAS011040 Hydrolase
PTM	Disulfide bond SAAS SAAS011040
Technical term	3D-structure PDB 4B7J PDB 4B7M PDB 4B7N
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral component of membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	294	1	Calcium; via carbonyl oxygen PDB 4B7J PDB 4B7M PDB 4B7N
	Metal binding	298	1	Calcium; via carbonyl oxygen PDB 4B7J PDB 4B7M PDB 4B7N
	Metal binding	324	1	Calcium PDB 4B7J PDB 4B7M PDB 4B7N
	Metal binding	342	1	Calcium; via carbonyl oxygen PDB 4B7J PDB 4B7M PDB 4B7N
	Metal binding	344	1	Calcium; via carbonyl oxygen PDB 4B7J PDB 4B7M PDB 4B7N
	Binding site	150	1	Phosphate 1 PDB 4B7M
	Binding site	199	1	Phosphate 1; via amide nitrogen PDB 4B7M
	Binding site	293	1	Phosphate 2 PDB 4B7M
	Binding site	368	1	Phosphate 2 PDB 4B7M
	Binding site	402	1	Phosphate 2 PDB 4B7M
	Binding site	455	1	Phosphate 1 PDB 4B7M
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) PDB 4B7M PDB 4B7N
	Glycosylation	146	1	N-linked (GlcNAc...) PDB 4B7M PDB 4B7N
	Glycosylation	235	1	N-linked (GlcNAc...) PDB 4B7M PDB 4B7N

Sequences

	Sequence		Length	Mass (Da)
	F8UU09 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: 48F0F60CD8119D39	FASTA	469	51,656
	10 20 30 40 50 60 MNPNQKIITI GSVCMTIGMA NLILQIGNII SIWISHSIQL GNQNQIETCN QSVITYENNT 70 80 90 100 110 120 WVNQTYVNIS NTNFAAGQSV VSVKLAGNSS LCPVSGWAIY SKDNSIRIGS KGDVFVIREP 130 140 150 160 170 180 FISCSPLECR TFFLTQGALL NDKHSNGTIK DRSPYRTLMS CPIGEVPSPY NSRFESVAWS 190 200 210 220 230 240 ASACHDGINW LTIGISGPDN GAVAVLKYNG IITDTIKSWR NNRLRTQESE CACVNGSCFT 250 260 270 280 290 300 VMTDGPSDGQ ASYKIFRIEK GKIVKSVEMN APNYHYEECS CYPDSSEITC VCRDNWHGSN 310 320 330 340 350 360 RPWVSFNQNL EYQIGYICSG IFGDNPRPND KTGSCGPVSS NGANGVKGFS FKYGNGVWIG 370 380 390 400 410 420 RTKSISSRNG FEMIWDPNGW TGTDNDFSIK QDIVGINEWS GYSGSFVQHP ELTGLDCIRP 430 440 450 460 CFWVELIRGR PKENTIWTSG SSISFCGVNS DTVGWSWPDG AELPFTIDK

References

[1]

"Multidrug Resistant 2009 A/H1N1 Influenza Clinical Isolate with a Neuraminidase I223R Mutation Retains Its Virulence and Transmissibility in Ferrets." van der Vries E., Veldhuis Kroeze E.J., Stittelaar K.J., Linster M., Van der Linden A., Schrauwen E.J., Leijten L.M., van Amerongen G., Schutten M., Kuiken T., Osterhaus A.D., Fouchier R.A., Boucher C.A., Herfst S. PLoS Pathog. 7:E1002276-E1002276(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: A/Netherlands/2631_1202/2010 EMBL AEE69001.1.

[2]

"H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis." van der Vries E., Collins P.J., Vachieri S.G., Xiong X., Liu J., Walker P.A., Haire L.F., Hay A.J., Schutten M., Osterhaus A.D., Martin S.R., Boucher C.A., Skehel J.J., Gamblin S.J. PLoS Pathog. 8:e1002914-e1002914(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) IN COMPLEX WITH CALCIUM AND PHOSPHATE, GLYCOSYLATION AT ASN-88; ASN-146 AND ASN-235.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

JF906185 Viral cRNA. Translation: AEE69001.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4B7J	X-ray	2.42	A	1-469	[»]
4B7M	X-ray	2.50	A/B	1-469	[»]
4B7N	X-ray	2.84	A	1-469	[»]

ModBase

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MobiDB

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

Gene3D

2.120.10.10. 1 hit.

InterPro

IPR001860. Glyco_hydro_34.
IPR011040. Sialidases.
[Graphical view]

Pfam

PF00064. Neur. 1 hit.
[Graphical view]

SUPFAM

SSF50939. SSF50939. 1 hit.

ProtoNet

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Entry information

Entry name

F8UU09_9INFA

Accession

Primary (citable) accession number: F8UU09

Entry history

Integrated into UniProtKB/TrEMBL:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	February 19, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)